Hydrogen-fueled diesel engine without timed ignition

Experiments were carried out to investigate the feasibility of converting a diesel engine to hydrogen-fueled operation without providing a timed ignition system. Use was made of a glow plug and a multiple-strike spark plug. The work was done with an ASTM-CFR engine, the diesel head of which was modified to resemble the geometry of a D399 series Caterpillar diesel engine. Engine speed was 1240 rev/min. The glow plug was found to provide reliable ignition and smooth engine operation. It caused the hydrogen to ignite almost immediately upon the start of injection. Indicated mean effective pressures were on the order of 1.3φ MPa for equivalence ratios φ between 0.1 and 0.4 at a compression ratio of 18. This is significantly higher than the corresponding result obtained with diesel oil (about 0.6 φ MPa for 0.3 < ? < 0·9). Indicated thermal efficiencies were on the order of 0.4 for hydrogen and 0.20–0.25 for diesel oil. Operation with the multiple-strike spark system yielded similar values for IMEP and efficiency, but gave rise to large cycle-to-cycle variations in the delay between beginning of injection and ignition. Large ignition delays were associated with large amplitude pressure waves in the combustion chamber. The measured NO_χ concentrations in the exhaust gas were of the order of 50–100 ppm. This is significantly higher than the corresponding results obtained with premixed hydrogen and air at low equivalence ratios. Compression ignition could not be achieved even at a compression ratio of 29.     

Random or systematic sampling to detect a localised microbial contamination within a batch of food

Pathogenic microorganisms are known to be distributed heterogeneously in food products that are solid, semi-solid or powdered, like for instance peanut butter, cereals, or powdered milk. This complicates effective detection of the pathogens by sampling. Two-class sampling plans, which are deployed when the health hazard is severe and direct, specify how many samples have to be drawn. In order to take a representative sample, the sampling strategy is important, especially when the microorganisms are distributed heterogeneously or localised.This theoretical study shows the impact of random versus systematic sampling on the probability to detect localised microbial contamination in a batch of food. A statistical model was used to compare these sampling strategies. The microbial contamination was modelled as being present in one specific localised fraction of the batch in which the cells were randomly distributed, while no cells were present in the remaining part of the batch.The probability that the entire sampling scheme contains at least one cell was calculated for various numbers of samples drawn either randomly or systematically and was shown to depend on the size of the contaminated fraction, the microbial concentrations, and the number of samples drawn. The probability of detection was either equal or higher for systematic sampling as compared to random sampling. The maximal improvement in probability of detection was 0.37, when the sampling interval was equal to the size of the contaminated fraction, meaning that exactly one systematic sample hits the contaminated fraction. In those cases where the size of the contaminated fraction can be estimated, this study may assist in selecting the sampling strategy that is most optimal regarding probability of detection.     

Environmental comparison of biobased chemicals from glutamic acid with their petrochemical equivalents

Glutamic acid is an important constituent of waste streams from biofuels production. It is an interesting starting material for the synthesis of biobased chemicals, thereby decreasing the dependency on fossil fuels. The objective of this paper was to compare the environmental impact of four biobased chemicals from glutamic acid with their petrochemical equivalents, that is, N-methylpyrrolidone (NMP), N-vinylpyrrolidone (NVP), acrylonitrile (ACN), and succinonitrile (SCN). A consequential life cycle assessment was performed, wherein glutamic acid was obtained from sugar beet vinasse. The removed glutamic acid was substituted with cane molasses and ureum. The comparison between the four biobased and petrochemical products showed that for NMP and NVP the biobased version had less impact on the environment, while for ACN and SCN the petrochemical version had less impact on the environment. For the latter two an optimized scenario was computed, which showed that the process for SCN can be improved to a level at which it can compete with the petrochemical process. For biobased ACN large improvements are required to make it competitive with its petrochemical equivalent. The results of this LCA and the research preceding it also show that glutamic acid can be a building block for a variety of molecules that are currently produced from petrochemical resources. Currently, most methods to produce biobased products are biotechnological processes based on sugar, but this paper demonstrates that the use of amino acids from low-value byproducts can certainly be a method as well.     

Prevalence of Clostridium difficile in retailed meat in the Netherlands

Recent reports indicate that a large proportion of community-acquired Clostridium difficile infections (CA-CDI) are not linked to recent antibiotic therapy, older age, significant comorbidity or previous hospitalization. Possible community sources for CA-CDI include animals and food, and therefore a surveillance study on the prevalence of C. difficile in meat was performed. Samples of different meat species were collected from the retail trade and analyzed for the presence of C. difficile using a method that included selective enrichment in C. difficile broth, subsequent alcohol shock-treatment and plating onto C. difficile selective medium. C. difficile isolates were tested for the presence of toxin genes and were typed using PCR ribotyping. Of 500 samples tested, 8 (1.6%) were positive for the presence of C. difficile: 1 from lamb (6.3%) and 7 from chicken meat (2.7%). The isolated strains belonged to PCR ribotypes different from those that are currently most frequently found in patients with CDI in the Netherlands, except for C. difficile PCR ribotype 001 which was found in one chicken meat sample. This observation suggests that other matrices than meat may serve as a source for CA-CDI.     

Genetic vaccination against the melanocyte lineage-specific antigen gp100 induces cytotoxic T lymphocyte-mediated tumor protection

Melanocyte lineage-specific antigens, such as gp100, have been shown to induce both cellular and humoral immune responses against melanoma. Therefore, these antigens are potential targets for specific antimelanoma immunotherapy. A novel approach to induce both cellular and humoral immunity is genetic vaccination, the injection of antigen-encoding naked plasmid DNA. In a mouse model, we investigated whether genetic vaccination against the human gp100 antigen results in specific antitumor immunity. The results demonstrate that vaccinated mice were protected against a lethal challenge with syngeneic B16 melanoma-expressing human gp100, but not control-transfected B16. Both cytotoxic T cells and IgG specific for human gp100 could be detected in human gp100-vaccinated mice. However, only adoptive transfer of spleen-derived lymphocytes, not of the serum, isolated from protected mice was able to transfer antitumor immunity to nonvaccinated recipients, indicating that CTLs are the predominant effector cells. CTI, lines generated from human gp100-vaccinated mice specifically recognized human gp100. Interestingly, one of the CTL lines cross-reacted between human and mouse gp100, indicating the recognition of a conserved epitope. However, these CTLs did not appear to be involved in the observed tumor protection. Collectively, our results indicate that genetic vaccination can result in a potent antitumor response in vivo and constitutes a potential immunotherapeutic strategy to fight cancer.     

T cell apoptosis in human heart allografts: association with lack of co-stimulation?

It is unclear whether the intracardial immune reactivity after heart transplantation influences the peripheral immunological status (activation or nonresponsiveness) of the patient. Co-stimulation and activation-induced cell death (AICD) or apoptosis play an important role in determining the balance between lymphocyte reactivity and nonreactivity. Therefore, we studied the expression of co-stimulatory molecules and the process of apoptosis in biopsies of human heart allografts, using immunohistochemistry. Although a normal expression of co-stimulatory molecules on antigen-presenting cells was observed, the expression of their counter-structures on T cells was absent. This may be due to chronic T cell activation, which can lead to the induction of apoptosis via the Fas/Fas ligand pathway. In the infiltrates, a considerable percentage of the lymphocytes, but not the macrophages, were apoptotic. Apoptosis was confirmed by DNA fragmentation analysis. Increased numbers of Bax-expressing versus decreased numbers of Bcl2-expressing lymphocytes in comparison with normal lymphoid tissue confirmed a imbalance in favor of apoptosis. Apoptosis was biased towards CD4+ T cells (65.7% versus 26.6% in CD8+ T cells). Fas was expressed on most of the infiltrating cells. Fas ligand expression was also observed, not only on most of the T cells but also on all macrophages. Because macrophages were often detected in close contact with T cells, they may play a role in T cell regulation via the Fas/Fas ligand pathway. This study indicates that, during rejection, not only is tissue damage induced by infiltrating T cells, but also the infiltrating lymphocytes themselves are actively down-regulated (eg, AICD) by one another and by macrophages in the infiltrate. This regulatory process may affect the immunological status of the patient after heart transplantation.