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181.
C.F.M. Vermeulen 《Journal of Logic, Language and Information》2000,9(3):273-311
This paper is concerned with the structure of texts in which aproof is presented. Some parts of such a text are assumptions, otherparts are conclusions. We show how the structural organisation of thetext into assumptions and conclusions helps to check the validity of theproof. Then we go on to use the structural information for theformulation of proof rules, i.e., rules for the (re-)construction ofproof texts. The running example is intuitionistic propositional logicwith connectives , and. We give new proofs of some familiar results aboutthe proof theory of this logic to indicate how the new techniques workout. 相似文献
182.
Ivan Vermeulen Sander Bohte Koye Somefun Han La Poutré 《Service Oriented Computing and Applications》2007,1(3):185-196
We present a dynamic and distributed approach to the hospital patient scheduling problem, in which patients can have multiple
appointments that have to be scheduled to different resources. To efficiently solve this problem we develop a multi-agent
Pareto-improvement appointment exchanging algorithm: MPAEX. It respects the decentralization of scheduling authorities and
continuously improves patient schedules in response to the dynamic environment. We present models of the hospital patient
scheduling problem in terms of the health care cycle where a doctor repeatedly orders sets of activities to diagnose and/or
treat a patient. We introduce the Theil index to the health care domain to characterize different hospital patient scheduling
problems in terms of the degree of relative workload inequality between required resources. In experiments that simulate a
broad range of hospital patient scheduling problems, we extensively compare the performance of MPAEX to a set of scheduling
benchmarks. The distributed and dynamic MPAEX performs almost as good as the best centralized and static scheduling heuristic,
and is robust for variations in the model settings.
A preliminary version of this work has appeared as [1]. 相似文献
183.
Daniel Shin Nicole D. Frane Ryan M. Brecht Jesse Keeler Prof. Rajesh Nagarajan 《Chembiochem : a European journal of chemical biology》2015,16(18):2651-2659
Quorum sensing is cell‐to‐cell communication that allows bacteria to coordinate attacks on their hosts by inducing virulent gene expression, biofilm production, and other cellular functions, including antibiotic resistance. AHL synthase enzymes synthesize N‐acyl‐l ‐homoserine lactones, commonly referred to as autoinducers, to facilitate quorum sensing in Gram‐negative bacteria. Studying the synthases, however, has proven to be a difficult road. Two assays, including a radiolabeled assay and a colorimetric (DCPIP) assay are well‐documented in literature to study AHL synthases. In this paper, we describe additional methods that include an HPLC‐based, C?S bond cleavage and coupled assays to investigate this class of enzymes. In addition, we compare and contrast each assay for both acyl‐CoA‐ and acyl‐ACP‐utilizing synthases. The expanded toolkit described in this study should facilitate mechanistic studies on quorum sensing signal synthases and expedite discovery of antivirulent compounds. 相似文献
184.
185.
M Cecilia N Nunes Jeffrey K Brecht Alcina MMB Morais Steven A Sargent 《Journal of the science of food and agriculture》2006,86(2):180-190
‘Chandler’, ‘Oso Grande’ and ‘Sweet Charlie’ strawberry cultivars were harvested at four stages of color development ranging from color break to full red and stored in the dark for 8 days at 1 °C. Changes in the physical and chemical characteristics of fruit during storage were compared with those occurring during development in the field. Strawberry fruit harvested at the three‐quarters colored and full red stages continued their development and ripening during storage. However, fruit harvested at the color break and half‐colored stages did not develop like those ripened in the field. Strawberries harvested at the three‐quarters colored stage developed the same pH, acidity, soluble solids, ascorbic acid and total phenolics content during storage as the at‐harvest levels present in strawberries harvested at the full red stage. In addition, three‐quarters colored strawberries were as firm and red after storage as the full red stage fruit were at the time of harvest. Thus, strawberries harvested at the three‐quarters colored stage can be stored for a longer period than strawberries harvested full red while retaining better color and firmness. Copyright © 2005 Society of Chemical Industry 相似文献
186.
Rob J. Vandebriel Sylvie Remy Jolanda P. Vermeulen Evelien G. E. Hurkmans Kirsten Kevenaar Neus G. Bastús Beatriz Pelaz Mahmoud G. Soliman Victor F. Puntes Wolfgang J. Parak Jeroen L. A. Pennings Inge Nelissen 《International journal of molecular sciences》2022,23(10)
The widespread and increasing use of engineered nanomaterials (ENM) increases the risk of human exposure, generating concern that ENM may provoke adverse health effects. In this respect, their physicochemical characteristics are critical. The immune system may respond to ENM through inflammatory reactions. The NLRP3 inflammasome responds to a wide range of ENM, and its activation is associated with various inflammatory diseases. Recently, anisotropic ENM have become of increasing interest, but knowledge of their effects on the immune system is still limited. The objective of the study was to compare the effects of gold ENM of different shapes on NLRP3 inflammasome activation and related signalling pathways. Differentiated THP-1 cells (wildtype, ASC- or NLRP3-deficient), were exposed to PEGylated gold nanorods, nanostars, and nanospheres, and, thus, also different surface chemistries, to assess NLRP3 inflammasome activation. Next, the exposed cells were subjected to gene expression analysis. Nanorods, but not nanostars or nanospheres, showed NLRP3 inflammasome activation. ASC- or NLRP3-deficient cells did not show this effect. Gene Set Enrichment Analysis revealed that gold nanorod-induced NLRP3 inflammasome activation was accompanied by downregulated sterol/cholesterol biosynthesis, oxidative phosphorylation, and purinergic receptor signalling. At the level of individual genes, downregulation of Paraoxonase-2, a protein that controls oxidative stress, was most notable. In conclusion, the shape and surface chemistry of gold nanoparticles determine NLRP3 inflammasome activation. Future studies should include particle uptake and intracellular localization. 相似文献
187.
Maarten Baele An Vermeulen Frédéric Leloup Dimitri Adons Roos Peeters Frank Devlieghere Bruno De Meulenaer Peter Ragaert 《Packaging Technology and Science》2020,33(3):99-111
Since more and more pressure is exerted to reduce the use of plastic packaging materials, optimizing the use of food packaging is opportune. The aim of this study was to evaluate the combined effect of packaging materials, spanning a range of oxygen transmission rates (OTR), and retail illumination, on the microbial shelf life and safety of refrigerated ready-to-heat foods. Cooked potato slices were packaged in OPA/PP bags with a high OTR (28.85 ccO2/m2/d) and OPA-EVOH/PP bags with a low OTR (6.57 ccO2/m2/d). Cooked composite meals were packaged in tray and foil combinations, also spanning a range of OTR: PP trays (2.09 ccO2/tray/d) with OPA/PP foils (28.85 ccO2/m2/d), PP trays with OPA-EVOH/PP (6.57 ccO2/m2/d) foils, and PET trays (0.07 ccO2/tray/d) with PET top foil (32.86 ccO2/m2/d) . The packages were stored in a dark environment, or under fluorescent or LED light. Due to the rapid growth of lactic acid bacteria, the microbial shelf life of both food products was largely unaffected by the type of barrier. Illumination at 1000 lux for 12 hours per day led to temperature differences significantly affecting microbial growth. Based on the results, it could be concluded that re-evaluating packaging material choices for these foods may prove valuable, since the use of high-barrier multilayer packaging materials may be considered as a case of overpackaging. 相似文献
188.
189.
Brecht M.R. Donckels Dirk J.W. De PauwPeter A. Vanrolleghem Bernard De Baets 《Chemometrics and Intelligent Laboratory Systems》2012,110(1):20-31
The problem of model discrimination arises when several models are proposed to describe one and the same process, a situation encountered in many research fields. To identify the best model from the set of rival models, it may be necessary to collect new information about the process, and thus additional experiments have to be performed. Several approaches have been described in literature to design optimal discriminatory experiments. The anticipatory approach is one of them and is very appealing from a conceptual point of view because the expected information content of the newly designed experiment is considered, even before the experiment is performed (anticipatory design). In this paper, the performance of this approach is evaluated by comparing it with the performance of other, established approaches to optimal experimental design for model discrimination. To conduct this comparison four performance measures were defined: (1) whether the most appropriate model could be identified, (2) the number of additional experiments that have to be designed and performed to achieve model discrimination, (3) the quality of the parameter estimates of the model that is eventually identified as the most appropriate one, and (4) the rate at which the inadequate models are identified. The results clearly indicate that the anticipatory approach has its benefits and may be the preferred approach in many applications in (bio)chemical engineering and in-silico biology. 相似文献
190.