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141.
Pautler BG Woods GC Dubnick A Simpson AJ Sharp MJ Fitzsimons SJ Simpson MJ 《Environmental science & technology》2012,46(7):3753-3761
Glaciers and ice sheets are the second largest freshwater reservoir in the global hydrologic cycle, and the onset of global climate warming has necessitated an assessment of their contributions to sea-level rise and the potential release of nutrients to nearby aquatic environments. In particular, the release of dissolved organic matter (DOM) from glacier melt could stimulate microbial activity in both glacial ecosystems and adjacent watersheds, but this would largely depend on the composition of the material released. Using fluorescence and (1)H NMR spectroscopy, we characterize DOM at its natural abundance in unaltered samples from a number of glaciers that differ in geographic location, thermal regime, and sample depth. Parallel factor analysis (PARAFAC) modeling of DOM fluorophores identifies components in the ice that are predominantly proteinaceous in character, while (1)H NMR spectroscopy reveals a mixture of small molecules that likely originate from native microbes. Spectrofluorescence also reveals a terrestrial contribution that was below the detection limits of NMR; however, (1)H nuclei from levoglucosan was identified in Arctic glacier ice samples. This study suggests that the bulk of the DOM from these glaciers is a mixture of biologically labile molecules derived from microbes. 相似文献
142.
Brent M. Wilson Donald L. Johnson Hans Van Tilburg Matthew A. Russell Larry E. Murphy James D. Carr Robert J. De Angelis David L. Conlin 《JOM Journal of the Minerals, Metals and Materials Society》2007,59(10):14-18
The assessment of corrosion on the USS Arizona included the pioneering development of a minimum-impact cost-effective technique
to determine the corrosion rate of steel-hulled shipwrecks in seawater. The technique, with potential application worldwide,
is illustrated in this paper with the application to a World War II Japanese midget submarine submerged in deep waters off
the Oahu, Hawaii, coast. 相似文献
143.
The fixation of cells of petiolar hairs of tomato was monitored by phase contrast and Nomarski interference contrast microscopy. Because the cuticle covering these hairs is relatively impermeable some fixatives enter only the base of the hair so that a ‘fixation front’ can be followed through a single cell. The basal end of such a cell may be immobilized by the fixative while the apical end is still streaming. A variety of fixatives were tested at room temperature and at 273–278 K. All procedures tested failed to stabilize a pleomorphic canalicular system that is a dominant feature of these cells so that no clue to the presence of this system can be seen in sectioned material. It is therefore very desirable that images of fixed cells be compared to the structure of similar cells which are still alive. 相似文献
144.
145.
Wei Meifen; Ku Tsun-Yao; Russell Daniel W.; Mallinckrodt Brent; Liao Kelly Yu-Hsin 《Canadian Metallurgical Quarterly》2008,55(4):451
This study examined 3 coping strategies (reflective, suppressive, and reactive), along with self-esteem, as moderators of the relation between perceived discrimination and depressive symptoms. International students (N = 354) from China, India, Korea, Taiwan, and Hong Kong provided data via an online survey. The role of perceived general stress was statistically controlled. Hierarchical regression analyses indicated a significant direct effect of perceived discrimination, a significant 2-way interaction of perceived discrimination and suppressive coping, and a significant 3-way interaction of perceived discrimination, reactive coping, and self-esteem in predicting depressive symptoms. An increased tendency to use suppressive coping appeared to strengthen the association between perceived discrimination and depressive symptoms. In contrast, the association between perceived discrimination and depressive symptoms was not significant when reactive coping was infrequently used, but only for students with relatively high self-esteem. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
146.
Microfluidic purification and preconcentration of mRNA by flow-through polymeric monolith 总被引:1,自引:0,他引:1
Efficient and rapid isolation of mRNA is important in the field of genomics as well as in the clinical and pharmaceutical arena. We have developed UV-initiated methacrylate-based porous polymer monoliths (PPM) for microfluidic trapping and concentration of eukaryotic mRNA. PPM are cast-to-shape and are tunable for functionalization using a variety of amine-terminated molecules. Efficient isolation of eukaryotic mRNA from total RNA was first mathematically modeled and then achieved using PPM in capillaries. Purification protocols using oligo dT's, locked nucleic acid substituted dT's, and tetramethylammonium chloride salts were characterized. mRNA yield and purity were compared with mRNA isolated by commercial kits with statistically equivalent yields and purities (determined by qPCR ratio of 18s rRNA and Gusb mRNA markers). Even after extracting 16 microg of mRNA from 315 microg of total RNA, the 0.4-microL volume monolith showed no signs of saturation. Elution volumes were below 20 microL with concentrations up to 1 microg/microL. In addition, the polymeric material exhibited exceptional stability in a range of conditions (pH, temperature, dryness) and was stable for a period of months. All of these characteristics make porous polymer monoliths good candidates for potential microfluidic sample preconcentrators and purifiers. 相似文献
147.
Todd J. Freeborn Ahmed S. Elwakil Brent Maundy 《Circuits, Systems, and Signal Processing》2016,35(6):1973-1982
In this paper we use a least-squares fitting routine to approximate the stopband ripple characteristics of fractional-order inverse Chebyshev lowpass filters which have fractional-order zeros and poles. MATLAB simulations of \((1+\alpha )\)-order lowpass filters with fractional steps from \(\alpha =0.1\) to \(\alpha =0.9\) are given as examples. SPICE simulations of 1.2-, 1.5-, and 1.8-order lowpass filters and experimental results of a 1.5-order filter using approximated fractional-order capacitors in a Multiple-Input Biquad circuit validate the implementation of these circuits. 相似文献
148.
Roadmapping the Technological Future of Electricity 总被引:1,自引:0,他引:1
A future-scanning collaboration orchestrated by the Electric Power Research Institute has sought to illuminate the technologies whose development will be needed to attain the “2 percent” solution of improvements in economic productivity, energy efficiency and agricultural productivity necessary for global sustainability. 相似文献
149.
150.
Ribeka Takahashi David T. Fullwood Brent L. Adams 《Metallurgical and Materials Transactions A》2014,45(10):4710-4722
This study employs a novel stress-based Hybrid–Bishop–Hill yield model approach to evaluate the yield surface of oxygen-free electronic copper samples. The local yield surface is determined from three parameters of crystal orientation and one parameter of geometrically necessary dislocation (GND). All four local state variables can be rapidly determined by analysis of measured electron backscatter diffraction patterns. Estimates for the polycrystalline yield surface are obtained by standard averaging procedures. The shape of the yield surface is most influenced by the texture of the material, while the volume of the envelope scales with the average GND density. However, correlations between crystal orientation and GND content modify the yield surface shape and size. While correlations between GND density and crystal orientation are not strong for most copper samples, there are sufficient dependencies to demonstrate the benefits of the detailed four-parameter model. The four-parameter approach has potential for improving estimates of elastic-yield limit in all polycrystalline materials. 相似文献