Optical imaging for the new grammar of drug discovery

Optical technologies used in biomedical research have undergone tremendous development in the last decade and enabled important insight into biochemical, cellular and physiological phenomena at the microscopic and macroscopic level. Historically in drug discovery, to increase throughput in screening, or increase efficiency through automation of image acquisition and analysis in pathology, efforts in imaging were focused on the reengineering of established microscopy solutions. However, with the emergence of the new grammar for drug discovery, other requirements and expectations have created unique opportunities for optical imaging. The new grammar of drug discovery provides rules for translating the wealth of genomic and proteomic information into targeted medicines with a focus on complex interactions of proteins. This paradigm shift requires highly specific and quantitative imaging at the molecular level with tools that can be used in cellular assays, animals and finally translated into patients. The development of fluorescent targeted and activatable 'smart' probes, fluorescent proteins and new reporter gene systems as functional and dynamic markers of molecular events in vitro and in vivo is therefore playing a pivotal role. An enabling optical imaging platform will combine optical hardware refinement with a strong emphasis on creating and validating highly specific chemical and biological tools.     

Compact SCSRR-based quad-band antenna for Bluetooth,WiMAX, WLAN,and fixed-satellite services

This research describes the detailed analysis and design of the compact complex-free structural metamaterial quad band radiating element applicable for Bluetooth, WiMAX, WLAN, and fixed-satellite service. The radiating element designed is composed of an inner angle-rotated circular Split Ring Resonator (SRR) placed within the outer square-shaped SRR interlinked by a strip to design the multiband operational characteristics and is fed by a coplanar waveguide. Suggested radiating element is imprinted over the FR4 substrate material with the electrical dimension of 28 × 31.26 × 0.8 mm³. The initial outer-closed square ring offers dual-band operation by resonating at 2.8 and 8.5 GHz frequencies, and the incorporation of a circular SRR offers quad-band operation. The unique negative permeability feature of the proposed Square and Circular Split Ring Resonator (SCSRR) structure is extracted, and its band characteristics are analyzed. Results obtained from the simulated radiating element are validated with the fabricated antenna. The measured E plane pattern resembles a numeric eight shape, and the H plane pattern is omnidirectional. Suggested SCSRR antenna offers a gain of above 3.2 dBi in all the operating bands.     

Comparative Assessment of Crystallographic Phase Stability of Anatase and Rutile TiO2 at Dynamic Shock Wave Loaded Conditions

In the present research article, authors have experimentally evaluated the shock wave resistant properties of technologically potential materials of the anatase and the rutile phase TiO₂ nanoparticles at the dynamic shock wave loaded conditions. The shock wave resistant behavior has been quantitatively drawn utilizing the crystallographic phase stability of the test samples for which the required crystallographic information has been extracted from the powder XRD patterns. Based on our observed experimental results as well as the respective interpretations, it is strongly authenticated that Rutile TiO₂ NPs are suitable candidates for aerospace and defense industrial applications of materials fabrications because of the outstanding shock resistant properties than that of Anatase TiO₂ NPs which undergo the crystallographic phase transition of rutile-TiO₂ at shocked conditions.

     

Rational Design of Self-Assembling Artificial Proteins Utilizing a Micelle-Assisted Protein Labeling Technology (MAPLabTech): Testing the Scope**

Self-assembling artificial proteins (SAPs) have gained enormous interest in recent years due to their applications in different fields. Synthesis of well-defined monodisperse SAPs is accomplished predominantly through genetic methods. However, the last decade has witnessed the use of a few chemical technologies for this purpose. In particular, micelle-assisted protein labeling technology (MAPLabTech) has made huge progress in this area. The first generation MAPLabTech focused on site-specific labeling of the active-site residue of serine proteases to make SAPs. Further, this methodology was exploited for labeling of N-terminal residue of a globular protein to make functional SAPs. In this study, we describe the synthesis of novel SAPs by developing a chemical method for site-specific labeling of a surface-exposed cysteine residue of globular proteins. In addition, we disclose the synthesis of redox-sensitive SAPs and their systematic self-assembly and disassembly studies using size-exclusion chromatography. Altogether these studies further expand the scope of MAPLabTech in different fields such as vaccine design, targeted drug delivery, diagnostic imaging, biomaterials, and tissue engineering.