Inherent and apparent scattering properties of coated or uncoated spheres embedded in an absorbing host medium

The conventional Lorenz-Mie formalism is extended to the case for a coated sphere embedded in an absorbing medium. The apparent and inherent scattering cross sections of a particle, derived from the far field and near field, respectively, are different if the host medium is absorptive. The effect of absorption within the host medium on the phase-matrix elements associated with polarization depends on the dielectric properties of the scattering particle. For the specific cases of a soot particle coated with a water layer and an ice sphere containing an air bubble, the phase-matrix elements -P12/P11 and P33/P11 are unique if the shell is thin. The radiative transfer equation for a multidisperse particle system embedded within an absorbing medium is discussed. Conventional multiple-scattering computational algorithms can be applied if scaled apparent single-scattering properties are applied.     

In-channel electrochemical detection for microchip capillary electrophoresis using an electrically isolated potentiostat

A new electrode configuration for microchip capillary electrophoresis (CE) with electrochemical (EC) detection is described. This approach makes it possible to place the working electrode directly in the separation channel. The "in-channel" EC detection was accomplished without the use of a decoupler through the utilization of a specially designed, electrically isolated potentiostat. The effect of the working electrode position on the separation performance (in terms of plate height and peak skew) of poly(dimethylsiloxane)-based microchip CEEC devices was evaluated by comparing the more commonly used end-channel configuration with this new in-channel approach. Using catechol as the test analyte, it was found that in-channel EC detection decreased the total plate height by a factor of 4.6 and lowered the peak skew by a factor of 1.3. A similar trend was observed for the small, inorganic ion nitrite. Furthermore, a fluorescent and electrochemically active amino acid derivative was used to directly compare the separation performance of in-channel EC detection to that of a widely used laser-induced fluorescence (LIF) detection scheme. In this case, it was found that the plate height and peak skew for both detection schemes were essentially equal, and the separation performance of in-channel EC detection is comparable to LIF detection.     

Truck sizing models for recyclables pick-up

Growing public concerns about the environmental consequences of waste disposal have led to an increase in recycling efforts. In many communities, curbside collection programs have been instituted for collecting recyclables from households for transfer to material recovery facilities. In most programs the cost of collection is the dominant cost. Despite the high cost and environmental importance of collecting recyclables from households, most municipal collection systems are designed and operated without a clear understanding of what factors drive those costs and how they might be reduced. In this paper, models for determining truck configurations, as well as optimal compartment sizes for recyclables collection are developed. Constraints arising from the operation of the trucks are formulated, and solution schemes are presented. In addition, a procedure for optimally partitioning a vehicle used to collect source-separated material is discussed. Several scenario based planning approaches are developed. Use of the truck sizing models as an industry decision tool and the associated benefits of applying the truck sizing models and the solution methodology to a contract based collection program are also discussed.     

Relationships among team ability composition, team mental models, and team performance.

This study examined the relationship between the similarity and accuracy of team mental models and compared the extent to which each predicted team performance. The relationship between team ability composition and team mental models was also investigated. Eighty-three dyadic teams worked on a complex skill task in a 2-week training protocol. Results indicated that although similarity and accuracy of team mental models were significantly related, accuracy was a stronger predictor of team performance. In addition, team ability was more strongly related to the accuracy than to the similarity of team mental models and accuracy partially mediated the relationship between team ability and team performance, but similarity did not. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Assessing the potential of SeaWiFS and MODIS for estimating chlorophyll concentration in turbid productive waters using red and near-infrared bands

Bio-optical algorithms for remote estimation of chlorophyll-a concentration (Chl) in case-1 waters exploit the upwelling radiation in the blue and green spectral regions. In turbid productive waters other constituents, that vary independently of Chl, absorb and scatter light in these spectral regions. As a consequence, the accurate estimation of Chl in turbid productive waters has so far not been feasible from satellite sensors. The main purpose of this study was to evaluate the extent to which near-infrared (NIR) to red reflectance ratios could be applied to the Sea Wide Field-of-View Sensor (SeaWiFS) and the Moderate Imaging Spectrometer (MODIS) to estimate Chl in productive turbid waters. To achieve this objective, remote-sensing reflectance spectra and relevant water constituents were collected in 251 stations over lakes and reservoirs with a wide variability in optical parameters (i.e. 4 ≤ Chl ≤ 240 mg m^− 3; 18 ≤ Secchi disk depth ≤ 308 cm). SeaWiFS and MODIS NIR and red reflectances were simulated by using the in-situ hyperspectral data. The proposed algorithms predicted Chl with a relative random uncertainty of approximately 28% (average bias between − 1% and − 4%). The effects of reflectance uncertainties on the predicted Chl were also analyzed. It was found that, for realistic ranges of R_rs uncertainties, Chl could be estimated with a precision better than 40% and an accuracy better than ± 35%. These findings imply that, provided that an atmospheric correction scheme specific for the red-NIR spectral region is available, the extensive database of SeaWiFS and MODIS images could be used to quantitatively monitor Chl in turbid productive waters.     

Application of simulated annealing to the biclustering of gene expression data.

In a gene expression data matrix, a bicluster is a submatrix of genes and conditions that exhibits a high correlation of expression activity across both rows and columns. The problem of locating the most significant bicluster has been shown to be NP-complete. Heuristic approaches such as Cheng and Church's greedy node deletion algorithm have been previously employed. It is to be expected that stochastic search techniques such as evolutionary algorithms or simulated annealing might improve upon such greedy techniques. In this paper we show that an approach based on simulated annealing is well suited to this problem, and we present a comparative evaluation of simulated annealing and node deletion on a variety of datasets. We show that simulated annealing discovers more significant biclusters in many cases. Furthermore, we also test the ability of our technique to locate biologically verifiable biclusters within an annotated set of genes.     

An experimental investigation of ion-drag pump in a vertical andaxisymmetric configuration

An ion-drag pump in a vertical, axisymmetric configuration was built. Two pumping sections with 1 and 10 electrode pairs were constructed. The total length of the pumping loop with one electrode pair was 133.0 cm; with 10 electrode pairs it was 186.0 cm. The electrodes were designed from the fluid mechanics standpoint. The ions were injected into the fluid where the viscous shear stresses were the highest. The fluid used was dodecylbenzine, and all the experiments were conducted at 25°C. Pumping velocities as high as 33.4 cm/s (corresponding mass flow rate of 1.1 kg/s and total pressure generation of 111.9 Pa) at 25 kV have been achieved. The highest pump power efficiency obtained was 6.0%. The effect of the electrical conductivity level of dodecylbenzine on the pump performance was also studied. Increasing the conductivity level reduced the pumping velocity     

Effect of ischaemia and reperfusion on the intracellular concentration of taurine and glutamine in the hearts of patients undergoing coronary artery surgery

Taurine and glutamine are the most abundant intracellular free amino acids in mammalian hearts where changes in their intracellular concentrations are likely to influence a number of cellular activities. In this study we investigated the effects of ischaemia and reperfusion on the intracellular concentrations of taurine and glutamine in the hearts of patients undergoing coronary artery bypass surgery using cold crystalloid or cold blood cardioplegic solutions. Ischaemic arrest (30 min), using cold crystalloid cardioplegic solution (n = 19), decreased the intracellular concentrations (micromol/g wet weight) of taurine (from 9.8 +/- 0.8 to 7.7 +/- 0.7, P < 0.05) and glutamine (8.7 +/- 0.5 to 7.2 +/- 0.6). After 20 min of normothermic reperfusion the fall in taurine and glutamine was maintained (7.5 +/- 0.5 and 7.4 +/- 0.7 for taurine and glutamine respectively). Myocardial ischaemic arrest with cold blood cardioplegic solution (n = 16) did not cause a significant fall in tissue taurine or glutamine. However, on reperfusion there was a marked fall in the intracellular concentrations of taurine (9.4 +/- 0.5 to 6.5 +/- 0.7) and glutamine (8.0 +/- 0.7 to 5.8 +/- 0.4). The fall in amino acids was associated with a fall in ATP and a rise in tissue lactate. This work demonstrates that irrespective of the cardioplegic solution used to arrest the heart, there is a marked fall in tissue taurine and glutamine which may influence the extent of recovery following surgery. The fall in taurine is largely due to efflux whereas changes in glutamine are due to both transport and metabolism. Ischaemia, hypothermia and changes in the transmembrane concentration gradients are the likely factors responsible for the changes in tissue amino acids.     

Animating structure plots: Experiments with a PERQ

A PERQ single user computer can be used to animate structure plots, which is of great assistance when interpreting the results from finite element programs. Excellent appreciation may be obtained of a structure oscillating in complicated natural modes. The technique is also useful for studying deformations due to static forces.     

Analogues of the thrombin receptor tethered-ligand enhance mesangial cell proliferation

Thrombin stimulates cytosolic calcium mobilization and tritiated thymidine incorporation in rat glomerular mesangial cells. This effect may be mediated by a thrombin receptor similar to the receptor found in human platelets. In order to test this possibility, a series of analogues of the thrombin receptor peptide, SFLL-RNPNDKYEPF, was evaluated for their effects on mesangial cells. Analogues of the thrombin receptor peptide containing five, six, seven and 14 amino acids were as efficacious as thrombin with respect to calcium mobilization and thymidine incorporation, although they were significantly less potent. The dissimilarity in potency between thrombin and the thrombin receptor peptides is consistent with the kinetics of the proposed mechanism of action of the enzyme, since the cleavage by thrombin of its receptor results in a tethered ligand which is at a relatively high concentration compared to the free peptides in solution. Those thrombin receptor peptide analogues which showed decreased activity in platelets were tested in mesangial cells. Removal of serine at position one, N-acetylation, or replacement of the phenylalanine at position two with alanine resulted in analogues which were inactive in stimulating mesangial cell proliferation or calcium mobilization. In addition, those analogues which had no stimulatory effects in mesangial cells were not antagonists of SFLLRN-mediated calcium mobilization and thymidine incorporation in mesangial cells.