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131.
杂色云芝漆酶的分离、纯化和酶学特性研究 总被引:9,自引:3,他引:9
杂色云芝发酵液,经超滤、DEAE-Sephadex A250柱层析两步分离、纯化出两型漆酶。以愈创木酚与酶带在适当条件下反应呈棕红色谱带鉴定出漆酶谱带。两型漆酶分别命名为LacA和LacB。经测定两漆酶亚基的分子量分别为66.0kDa和30.7kDa;等电点pI分别为4.79和5.18;LacA的最适作用温度是40℃,LacB为50℃;保温2h, LacA的半失活温度为60℃,LacB为90℃;LacA和LacB催化愈创木酚的酶活最适pH值均为4.0; 二者的稳定pH值也都在2.5~5.5之间。LacA催化氧化愈创木酚的Km和Vmax分别为286.5molL-1和292.4nmol mL-1 min-1;LacB催化氧化愈创木酚的Km和Vmax分别为813.0mol L-1和127.4nmol mL-1 min-1。 相似文献
132.
Li‐Qiang Shen Zhi‐Kang Xu Qian Yang Hang‐Lin Sun Shu‐Yuan Wang You‐Yi Xu 《应用聚合物科学杂志》2004,92(3):1709-1715
Polyetherimide (PEI) was sulfonated by chlorosulfonic acid (CSA) in 1,2‐dichloroethane for the first time. With the increase of the CSA/PEI repeat unit ratio and/or reaction time, the ion‐exchange capacity (IEC) of the sulfonated polyetherimide (SPEI) increased accordingly. Water‐uptake testing and contact‐angle measurement showed that the hydrophilicity of the SPEI increases with the increase of the IEC. Membranes were fabricated from SPEI/PEI blends with different ratios. The morphologies of the blend membranes were examined by scanning electron microscopy, which showed that the membrane pore size is larger when SPEI with higher IEC was used. With the increase of SPEI ratio in the blend membranes, the membrane pore size also increased. The contact‐angle data of the membranes showed that the hydrophilicity of the blend membrane was elevated because of the sulfonate group on the SPEI molecular backbone. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 92: 1709–1715, 2004 相似文献
133.
Won Seok Lyoo Jeong Hyun Yeum Byung Chul Ji Han Do Ghim Sam Soo Kim Joon Ho Kim Ji Youl Lee Jinwon Lee 《应用聚合物科学杂志》2003,88(6):1482-1487
Water‐soluble high molecular weight (HMW) syndiotactic poly(vinyl alcohol) (s‐PVA) microfibrillar fibers were prepared by the saponification with various conditions such as amount of alkali solution, saponification temperature, and saponification concentration from copoly(vinyl pivalate (VPi)/vinyl acetate (VAc)) copolymerized using various VPi/VAc feed ratios. To produce s‐PVA microbrillar fibers having various water‐soluble temperatures for many industrial applications, the intrinsic viscosities, syndiotactic diad (S‐diad) contents, and degrees of saponification (DS)s of PVAs were finely controlled to 1.2–3.6 dL/g, 56.3–58.3%, and 91.4–98.3%, respectively. Through a series of experiments, it was found that the amount of alkali may alter the structure of the saponified polymers, primarily the DS, and the structural variation changes viscosity. That is, intrinsic viscosity was sharply decreased as the amount of alkali solution was increased. DS was increased with an increase in the amount of alkali solution. S‐diad content was increased with an increase in the VPi content. HMW s‐PVA microfibrillar fibers having S‐diad content of 56.3–58.3% prepared by the saponification of copoly(VPi/VAc) were completely soluble in water at 100°C. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 88: 1482–1487, 2003 相似文献
134.
Myung-Sup Jung Tae-Woo Lee Jingyu Hyeon-Lee Byung Hee Sohn In-Sun Jung 《Polymer》2006,47(8):2670-2676
Polyimide containing triphenylamine derivative (TPD-PI) was synthesized to prepare a polymer interlayer having insolubility in common nonpolar solvent for light-emitting polymers. N,N′-diphenyl-N,N′-bis(4-aminophenyl)-1,1-biphenyl-4,4′-diamine, as a new triphenylamine-containing diamine monomer, was synthesized by the palladium-catalyzed amination reaction between 4-nitrodiphenylamine and 4,4′-dibromobiphenyl and subsequent reduction of the nitro-intermediate. The TPD-PI was prepared from the synthesized diamine monomer and 4,4′-(hexafluoropropylidene)-diphthalic anhydride by the standard two-step polymerization method, which involved ring-opening polymerization and subsequent cyclodehydration. The structures and properties of the monomer and the resulting polyimide were characterized with 1H and 13C NMR, elemental analysis, gel permeation chromatography, UV-visible spectroscopy, etc. The TPD-PI is readily soluble in aprotic polar solvents such as N-methyl-2-pyrrolidinone and N,N-dimethylformamide and insoluble in nonpolar solvents such as toluene and xylene. The highest occupied molecular orbital (HOMO) level of the TPD-PI was measured to be 5.5 eV by a photoelectron spectrometer in air, and the band gap was calculated as 3.1 eV from the onset of UV-vis spectrum. The polymer light-emitting diode with the thin TPD-PI layer between a hole injection layer and an emitting polymer layer was fabricated to examine the performance of the polyimide as an polymer interlayer. Although the luminous efficiency of the device is lowered by the introduction of the TPD-PI interlayer, the lifetime of the device is improved. 相似文献
135.
为提高车牌识别的速度和准确性,在图像处理与车牌识别技术相结合的基础上,提出了一种基于实例推理(CBR)的车牌识别方法,实现CBR在车牌识别领域的应用。描述了基于CBR车牌识别过程,分析了将CBR应用于车牌识别原因和前景,并提出了图像基于实例检索的关键技术和研究方向. 相似文献
136.
定制非周期极化LiNbO3中多频率THz波的产生 总被引:2,自引:2,他引:0
描述一种非周期极化结构晶体中多频率太赫兹辐射波的产生.在此非周期结构的LiNbO3,超晶格中,基于飞秒脉冲二阶非线性光学整流效应,模拟多频率模式的太赫兹波形.通过选择最佳畴宽度,得到转换效率基本相等的3个主要太赫兹频率模式.该方法对多频率模式的个数无限制,且有助于多频率激光器中超晶格晶体的设计. 相似文献
137.
彩钢岩棉夹芯屋面板、墙面板节点性能研究 总被引:1,自引:0,他引:1
为了掌握彩钢岩棉夹芯屋面板、墙面板与檩条的螺钉连接节点抗拉破坏机理,采用理论分析、试验验证及有限元模拟相结合的方法研究了不同钢板厚度及芯材厚度时节点破坏情况.分析结果表明:屋面板节点连接破坏是由于钢板局部屈曲引起的,墙面板则是钢板被冲剪破坏造成的,同时,结合对有限元模拟结果的回归,提出了节点承载力设计公式,可为以后节点设计提供参考.增加岩棉刚度及其与侧向钢板的粘结,或增加钢板厚度对提高屋面板节点极限承载力起着重要作用. 相似文献
138.
J. H. Kim J. H. Park J. H. Kim T. V. Cao T. Y. Lee H. J. Ban K. Yang H. G. Kim P. B. Ha Y. H. Kim 《中南工业大学学报(英文版)》2009,16(4):621-628
A power management unit (PMU) chip supplying dual panel supply voltage, which has a low electro-magnetic interference (EMI)
characteristic and is favorable for miniaturization, is designed. A two-phase charge pump circuit using external pumping capacitor
increases its pumping current and works out the charge-loss problem by using bulk-potential biasing circuit. A low-power start-up
circuit is also proposed to reduce the power consumption of the band-gap reference voltage generator. And the ring oscillator
used in the ELVSS power circuit is designed with logic devices by supplying the logic power supply to reduce the layout area.
The PMU chip is designed with MagnaChip’s 0.25 μm high-voltage process. The driving currents of ELVDD and ELVSS are more than
50 mA when a SPICE simulation is done. 相似文献
139.
A multi-bit antifuse-type one-time programmable (OTP) memory is designed, which has a smaller area and a shorter programming
time compared with the conventional single-bit antifuse-type OTP memory. While the conventional antifuse-type OTP memory can
store a bit per cell, a proposed OTP memory can store two consecutive bits per cell through a data compression technique.
The 1 kbit OTP memory designed with Magnachip 0.18 μm CMOS (complementary metal-oxide semiconductor) process is 34% smaller
than the conventional single-bit antifuse-type OTP memory since the sizes of cell array and row decoder are reduced. And the
programming time of the proposed OTP memory is nearly 50% smaller than that of the conventional counterpart since two consecutive
bytes can be compressed and programmed into eight OTP cells at once. The layout area is 214 μm × 327 μm, and the read current
is simulated to be 30.4 μA.
Foundation item: Project supported by the 2nd Stage of Brain Korea; Project supported by the Korea Research Foundation 相似文献
140.
近年来,我国外向型经济发展较快,一些"休眠企业"逐渐浮出水面,给外汇管理工作带来了一定的难题,本文紧密联系实际工作,从"休眠企业"的类型、成因、危害性以及相应对策进行了简要的分析,力图通过制度、方法等有效途径最大程度上降低"休眠企业"的危害性,使外汇管理工作更好地为国民经济健康发展保驾护航。 相似文献