The influence of delayed light curing on the degree of conversion and polymerization contraction stress in dual-cured resin luting agents

The aim of the study was to assess the effect of delayed photo-initiation on the polymerization contraction stress (PCS) and degree of conversion (DC) of a dual-cure resin-luting agent. Thirty-five disk (6 mm × 1 mm) samples (n = 10 each group) of dual cure resin luting agent for PCS assessment were fabricated and polymerized using two illuminated quartz rods. Based on the delay in photo-initiation, 30 disks were divided among six groups [group A-0 min (min) delay, group B-2 min, group C-4 min, group D-6 min, group E-8 min and group F-10 min]. A non-photoinitiated group (group G – chemical cure – n = 5) was included as control. The PCS for all specimens was assessed using a Tensometer. For DC evaluation thirty-five specimens were divided into seven groups with delays in photo-initiation (group H-0 min, group I-2 min, group J-4 min, group K-6 min, group L-8 min and group M-10 min, group N-chemical cure). DC was assessed using attenuated total reflectance spectroscopic technique. Statistical comparison among groups was performed using analysis of variance (α = 0.05). The maximum and minimum PCS and DC values with delayed photo-initiation was observed in group-C (3.34 MPa) & group-F (2.44 MPa); and group-M (0.78 MPa) and group-H (0.55 MPa) respectively. Chemically cured samples showed the least PCS (group-G, 1.94) and DC (group-N, 0.53) values in their respective categories. PCS significantly decreased with delayed photo-initiation. A significant increase in DC was noticed when photo-initiation was delayed in the dual cure resin luting agent.     

Mass Transport in Shallow Turbulent Wake Flow by Planar Concentration Analysis Technique

A planar concentration analysis (PCA) system is used for observing the transport and mixing of a tracer mass in a shallow turbulent free-surface wake flow of a large cylindrical obstacle. The nonintrusive, fieldwise PCA measuring technique is applied to evaluate depth-averaged mass concentrations by making use of light attenuation due to absorption and scattering processes related to a dissolved tracer mass. The scalar fields are decomposed into a low-frequency quasiperiodic part, the coherent flow, and a randomly fluctuating part. From accompanying near-surface velocity measurements, large-scale coherent structures are identified and related to the coherent mass fields. This allows one to assess the role of the large-scale vortices for advection and diffusion in shallow wake flows. The time–mean wake flow displays a self-similar spanwise distribution both for mass and velocity. The longitudinal development of shallow wakes initially shows the growth of unbounded wakes; in the wake far field an attenuated behavior applies.     

Effects of Particle Crushing in Stress Drop-Relaxation Experiments on Crushed Coral Sand

Stress relaxation and stress drop-relaxation tests have been performed to complement a test series performed to study strain rate, creep, and stress drop-creep effects on crushed coral sand. Drained experiments with constant effective confining pressure of 200 kPa were performed in which triaxial specimens of crushed coral sand were loaded to initial stress differences of 500, 700, and 900 kPa, followed by stress drops of 0, 100, 200, 300, and 400 kPa at which points the axial strains were kept constant while the axial stress relaxation and the volumetric strains were observed. The stress drops produced delays in initiation of stress relaxation that were proportional with the magnitudes of the stress drops. The experiments show that sands do not exhibit classic viscous effects, and their behavior is indicated as “nonisotach,” while the typical viscous behavior of clay is termed “isotach.” Thus, there are significant differences in the time-dependent behavior patterns of sands and clay. A mechanistic picture of time effects in sands is proposed.     

An in vitro assay to evaluate competitive exclusion products for poultry

An in vitro assay was developed to measure the ability of competitive exclusion (CE) bacteria to protect Caco-2 and CRL-2117 epithelial cells from invasion by Salmonella Typhimurium. The proposed assay is needed to expedite the development of defined-flora CE products. The average significantly protective concentration of the commercial poultry-specific CE product Preempt was 4.05 log CFU/6.41 log human Caco-2 cells and 3.71 log CFU/6.89 log CFU chicken CRL-2117 cells, Enterococcus faecalis isolated from Preempt protected CRL-2117 cells, Escherichia coli isolates protected Caco-2 cells, Lactococcus lactis and Bacteroides distasonis isolates protected both cell lines, and three species of Lactobacillus isolates failed to protect either cell line. A defined mixture of 29 strains of bacteria similar to the constituents of Preempt protected both cell lines from Salmonella invasion at a concentration of 7.83 log CFU. The constituents of the defined CE culture were separated into mixtures of obligate (8.42 log CFU) and facultative (8.49 log CFU) anaerobes, which both protected the cell lines, suggesting that both types of bacteria were equally protective. Although not a substitute for in vivo testing, the in vitro CE assay is a rapid technique for the evaluation of bacterial mixtures for potential CE products.