Impact of black carbon in the extraction and mineralization of phenanthrene in soil

During the past century, increased biomass burning and fossil fuel consumption have drastically increased the input of black carbon (BC) into the environment, and that has been shown to influence the behavior of organic contaminants in soil. A study was conducted to investigate the effects of BC on the relationship between aqueous hydroxypropyl-beta-cyclodextrin (HPCD) extraction and microbial mineralization (bioaccessibility) of 14C-phenanthrene (10 mg kg(-1)) in four soils amended with 0, 0.1, 0.5, 1, 2.5, and 5% (% dry wt soil) activated charcoal, a type of BC. Mineralisation was monitored over 20 d incubation, within respirometric assays, using an inoculum containing a phenanthrene-degrading pseudomonad and compared to HPCD extraction (24 h) using 50 mM aqueous solution; analyses were conducted after 1, 25, 50, and 100 d soil-phenanthrene contact time. Statistical analyses revealed that for each soil the addition of BC led to significant (P < 0.001) reductions in both HPCD extractability and microbial mineralization. Linear correlations for BC concentrations of 0% (r2 = 0.95; slope = 0.89) and 0.1% (r2 = 0.67; slope = 0.95) revealed a highly significant (P < 0.01) relationship between HPCD extractability and total mineralization (20 d), indicating a direct prediction of phenanthrene bioaccessibility by HPCD. However, in soils amended with 0.5, 1, 2.5, and 5% BC exhibited r2 values ranging 0.51-0.13 and slopes of 2.19-12.73. This study has shown that BC strongly sorbs phenanthrene causing reductions in extractability and, to a lesser extent, bioaccessibility to degrading microorganisms.     

Decontamination of sugar syrup by pulsed light

The pulsed light produced by xenon flash lamps was applied to 65 to 67 °Brix sugar syrups artificially contaminated with suspensions of Saccharomyces cerevisiae and with spores of Bacillus subtilis, Geobacillus stearothermophilus, Alicyclobacillus acidoterrestris, and Aspergillus niger. The emitted pulsed light contained 18.5 % UV radiation. At least 3-log reductions of S. cerevisiae, B. subtilis, G. stearothermophilus, and A. acidoterrestris suspended in 3-mm-deep volumes of sugar syrup were obtained with a fluence of the incident pulsed light equal to or less than 1.8 J/cm(2), and the same results were obtained for B. subtilis and A. acidoterrestris suspended in 10-mm-deep volumes of sugar syrup. A. niger spores would require a more intense treatment; for instance, the maximal log reduction was close to 1 with a fluence of the incident pulsed light of 1.2 J/cm(2). A flowthrough reactor with a flow rate of 320 ml/min and a flow gap of 2.15 mm was designed for pulsed light treatment of sugar syrup. Using this device, a 3-log reduction of A. acidoterrestris spores was obtained with 3 to 4 pulses of incident pulsed light at 0.91 J/cm(2) per sugar syrup volume.     

Sporulation boundaries and spore formation kinetics of Bacillus spp. as a function of temperature, pH and a(w)

Sporulation niches in the food chain are considered as a source of hazard and are not clearly identified. Determining the sporulation environmental boundaries could contribute to identify potential sporulation niches. Spore formation was determined in a Sporulation Mineral Buffer. The effect of incubation temperature, pH and water activity on time to one spore per mL, maximum sporulation rate and final spore concentration was investigated for a Bacillus weihenstephanensis and a Bacillus licheniformis strain.     

Antimicrobial activity of foodborne Paenibacillus and Bacillus spp. against Clostridium botulinum

The saprophytic Paenibacillus and Bacillus spp. found in cooked chilled foods may have an effect on the growth of Clostridium botulinum, a major microbiological hazard, especially for pasteurized vacuum-packaged products. Culture supernatants of 200 strains of Paenibacillus and Bacillus strains isolated from commercial cooked chilled foods containing vegetables were screened for activity against C. botulinum type A, proteolytic type B, and type E strains in a well diffusion assay. Nineteen strains were positive against C. botulinum. Among those, seven Paenibacillus polymyxa strains showed the highest antibotulinal activity and the largest antimicrobial spectrum against C. botulinum strains. The antibotulinal activity was evaluated throughout the growth of a representative strain of the positive P. polymyxa strains. The antimicrobial activity was detected in the culture supernatant from late-log/early stationary phase of the bacteria, which occurred after 7 to 10 days of incubation at 10 degrees C and after 2 to 3 days at 20 degrees C in nutrient broth and in vegetable purées under aerobic or anaerobic conditions. In co-cultures with the positive strain of P. polymyxa in nutrient broth and vegetable purées, a C. botulinum type E strain was inhibited whenever P. polymyxa reached stationary phase and produced its antimicrobial activity before C. botulinum began its exponential growth phase. The antimicrobial activity of P. polymyxa against C. botulinum was attributed to the production of antimicrobial peptides resistant to high temperature and acidity. Other gram-positive and -negative bacteria (Escherichia coli, Streptococcus mutans, Leuconostoc mesenteroides, and Bacillus subtilis) were also sensitive to these antimicrobial peptides.     

Working chute behavior of feedlot cattle can be an indication of cattle temperament and beef carcass composition and quality

The relationship between working chute temperament and beef carcass quality traits were measured on 183 crossbred feedlot steers. Steers were weighed and sorted into 16 pens and weighed every 28 days. Temperament was evaluated as exit velocity (EV), chute score (CS), and catch score (CAPS). Tissue samples were collected for DNA profile including docility (IGENITY^®, Merial Limited, Duluth, GA, USA). Steers were harvested at 14 to 16 months of age (606 ± 52 kg). Exit velocity increased and CS and CAPS values declined over time. Steers receiving a lower CAPS score had more marbling (P = 0.02). Ribeye steaks from steers with a slow first recorded EV were more tender (P = 0.02) than steaks from faster EV steers. IGENITY^® docility index had a positive (P = 0.09) correlation (r = 0.15) with Warner–Bratzler shear force (WBS) indicating a higher docility index was loosely associated with tougher meat.     

Adaptation of Bacillus cereus,an ubiquitous worldwide-distributed foodborne pathogen,to a changing environment

Consequences of climate change on the ecology of pathogens are difficult to forecast. However changes affecting microorganisms will likely involve already known evolution or adaptation mechanisms. Bacillus cereus is a frequent cause of foodborne poisonings and is known as a soil borne bacterium. B. cereus may represent an interesting model to study the impact of climate change on foodborne pathogens. The B. cereus group (or B. cereus sensu lato) displays a wide diversity of strains recently distributed in seven major phylogenetic groups. B. cereus growth domains range from psychrotrophic to nearly thermophilic. Current climate selects B. cereus distribution: psychrotrophes are more common in cold areas, while mesophiles prevail in tropical soils. In response to external signals, B. cereus may adapt to changing environments by varied mechanisms. Some illustrations of the signal transduction systems (two-component systems, alternative σ factors) and of the mechanisms of B. cereus adaptation to major environmental factors (temperature, carbon source, redox potential and pH) are proposed. The environment of sporulation has an impact on spore properties; heat resistance is positively correlated with sporulation temperature. Surveillance needed to detect changes in the epidemiology of B. cereus foodborne poisonings as a consequence of climate change is discussed.     

Spatio-temporal models with errors in covariates: mapping Ohio lung cancer mortality

In estimating spatial disease patterns, as well as in related assessments of environmental equity, regional morbidity and mortality rate maps are widely used. Hierarchical Bayes methods are increasingly popular tools for creating such maps, since they permit smoothing of the fitted rates toward spatially local mean values, with more unreliable estimates (those arising in low-population regions) receiving more smoothing. In this paper we blend methods for spatial-temporal mapping with those for handling errors in covariates in a single hierarchical model framework. Estimated posterior distributions for the resulting highly-parameterized models are obtained via Markov chain Monte Carlo (MCMC) methods, which also play a key role in our approach to model evaluation and selection. We apply our approach to a data set of county-specific lung cancer rates in the state of Ohio during the period 1968-1988. Our model uses age-adjusted death rates, and incorporates recent information regarding smoking prevalence, population density, and the socio-economic status of the counties. This information is critical to understanding the role played by a certain depleted uranium fuel processing facility on the elevated lung cancer rates in the counties that neighbour it.     

Back-gated CMOS on SOIAS for dynamic threshold voltage control

The simultaneous reduction of power supply and threshold voltages for low-power design without suffering performance losses will eventually reach the limit of diminishing returns as static leakage power dissipation becomes a significant portion of the total power consumption. This is especially acute in systems that are idling most of the time. In order to meet the opposing requirements of high performance at reduced power supply voltage and low-static leakage power during idle periods, a dynamic threshold voltage control scheme is proposed. A novel Silicon-On-Insulator (SOI)-based technology called Silicon-On-Insulator-with-Active-Substrate (SOIAS) was developed whereby a back-gate is used to control the threshold voltage of the front-gate; this concept was demonstrated on a selectively scaled CMOS process implementing discrete devices and ring oscillators. For a 250 mV switch in threshold voltage, a reduction of 3-4 decades in subthreshold leakage current was measured