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排序方式: 共有103条查询结果,搜索用时 15 毫秒
51.
Carlotta Marasini Emanuela Jacchetti Manola Moretti Claudio Canale Oscar Moran Massimo Vassalli 《Microscopy research and technique》2013,76(7):723-732
Atomic force microscopy (AFM) proved to be able to obtain high‐resolution three‐dimensional images of single‐membrane proteins, isolated, crystallized, or included in reconstructed model membranes. The extension of this technique to native systems, such as the protein immersed in a cell membrane, needs a careful manipulation of the biological sample to meet the experimental constraints for high‐resolution AFM imaging. In this article, a general protocol for sample preparation is presented, based on the mechanical stretch of the cell membrane. The effectiveness for AFM imaging has been tested on the basis of an integrated optical and AFM approach and the proposed method has been applied to cells expressing cystic fibrosis transmembrane conductance regulator, a channel involved in cystic fibrosis, showing the possibility to identify and analyze single proteins in the plasma membrane. Microsc. Res. Tech. 76:723–732, 2013. © 2013 Wiley Periodicals, Inc. 相似文献
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Joseph A. Rothwell Pekka Keski‐Rahkonen Nivonirina Robinot Nada Assi Corinne Casagrande Mazda Jenab Pietro Ferrari Marie‐Christine Boutron‐Ruault Yahya Mahamat‐Saleh Francesca Romana Mancini Heiner Boeing Verena Katzke Tilman Kühn Katerina Niforou Antonia Trichopoulou Elisavet Valanou Vittorio Krogh Amalia Mattiello Domenico Palli Carlotta Sacerdote Rosario Tumino Augustin Scalbert 《Molecular nutrition & food research》2019,63(22)
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Marta Miola Alessandro Bistolfi Maria Carmen Valsania Carlotta Bianco Giacomo Fucale Enrica Verné 《Materials science & engineering. C, Materials for biological applications》2013,33(5):3025-3032
An in vitro study was carried out in order to investigate the antibiotic release mechanism and the antibacterial properties of commercially (Palacos® R + G and Palacos® LV + G) and manually (Palacos® R + GM and Palacos® LV + GM) blended gentamicin-loaded bone cements.Samples were characterized by means of scanning electron microscopy (SEM) and compression strength was evaluated. The antibiotic release was investigated by dipping sample in simulated body fluid (SBF) and periodically analyzing the solution by means of high pressure liquid chromatography (HPLC). Different antibacterial tests were performed to investigate the possible influence of blending technique on antibacterial properties.Only some differences were observed between gentamicin manually added and commercial ones, in the release curves, while the antibacterial effect and the mechanical properties seem to not feel the blending technique. 相似文献
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Suski JM Karkucinska-Wieckowska A Lebiedzinska M Giorgi C Szczepanowska J Szabadkai G Duszynski J Pronicki M Pinton P Wieckowski MR 《International journal of molecular sciences》2011,12(8):5373-5389
Reactive oxygen species (ROS) are wieldy accepted as one of the main factors of the aging process. These highly reactive compounds modify nucleic acids, proteins and lipids and affect the functionality of mitochondria in the first case and ultimately of the cell. Any agent or genetic modification that affects ROS production and detoxification can be expected to influence longevity. On the other hand, genetic manipulations leading to increased longevity can be expected to involve cellular changes that affect ROS metabolism. The 66-kDa isoform of the growth factor adaptor Shc (p66Shc) has been recognized as a relevant factor to the oxygen radical theory of aging. The most recent data indicate that p66Shc protein regulates life span in mammals and its phosphorylation on serine 36 is important for the initiation of cell death upon oxidative stress. Moreover, there is strong evidence that apart from aging, p66Shc may be implicated in many oxidative stress-associated pathologies, such as diabetes, mitochondrial and neurodegenerative disorders and tumorigenesis. This article summarizes recent knowledge about the role of p66Shc in aging and senescence and how this protein can influence ROS production and detoxification, focusing on studies performed on skin and skin fibroblasts. 相似文献
59.
Relation of allium vegetables intake with head and neck cancers: Evidence from the INHANCE consortium
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Carlotta Galeone Federica Turati Zuo‐Feng Zhang Valentina Guercio Alessandra Tavani Diego Serraino Paul Brennan Eleonora Fabianova Jola Lissowska Dana Mates Peter Rudnai Oxana Shangina Neonila Szeszenia‐Dabrowska Thomas L. Vaughan Karl Kelsey Michael McClean Fabio Levi Richard B. Hayes Mark P. Purdue Cristina Bosetti Hermann Brenner Claudio Pelucchi Yuan‐Chin Amy Lee Mia Hashibe Paolo Boffetta Carlo La Vecchia 《Molecular nutrition & food research》2015,59(9):1641-1650
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Stefania E. Sestito Dr. Paola Sperandeo Dr. Carlo Santambrogio Carlotta Ciaramelli Dr. Valentina Calabrese Prof. G. Enrico Rovati Luca Zambelloni Prof. Rita Grandori Prof. Alessandra Polissi Prof. Francesco Peri 《Chembiochem : a European journal of chemical biology》2014,15(5):734-742
Lipopolysaccharide (LPS), the main cell‐surface molecular constituent of Gram‐negative bacteria, is synthesized in the inner membrane (IM) and transported to the outer membrane (OM) by the Lpt (lipopolysaccharide transport) machinery. Neosynthesized LPS is first flipped by MsbA across the IM, then transported to the OM by seven Lpt proteins located in the IM (LptBCFG), in the periplasm (LptA), and in the OM (LptDE). A functional OM is essential to bacterial viability and requires correct placement of LPS in the outer leaflet. Therefore, LPS biogenesis represents an ideal target for the development of novel antibiotics against Gram‐negative bacteria. Although the structures of Lpt proteins have been elucidated, little is known about the mechanism of LPS transport, and few data are available on Lpt–LPS binding. We report here the first determination of the thermodynamic and kinetic parameters of the interaction between LptC and a fluorescent lipo‐oligosaccharide (fLOS) in vitro. The apparent dissociation constant (Kd) of the fLOS–LptC interaction was evaluated by two independent methods. The first was based on fLOS capture by resin‐immobilized LptC; the second used quenching of LptC intrinsic fluorescence by fLOS in solution. The Kd values by the two methods (71.4 and 28.8 μm, respectively) are very similar, and are of the same order of magnitude as that of the affinity of LOS for the upstream transporter, MsbA. Interestingly, both methods showed that fLOS binding to LptC is mostly irreversible, thus reflecting the fact that LPS can be released from LptC only when energy is supplied by ATP or in the presence of a higher‐affinity LptA protein. A fluorescent glycolipid was synthesized: this also interacted irreversibly with LptC, but with lower affinity (apparent Kd=221 μM ). This compound binds LptC at the LPS binding site and is a prototype for the development of new antibiotics targeting LPS transport in Gram‐negative bacteria. 相似文献