A Y-channel design for improving zeta potential and surface conductivity measurements using the current monitoring method

This study presents a new Y-channel design for measuring the zeta potential and surface conductance of a solid-liquid pairing using the current monitoring technique. The new design improves the throughput and reliability of the testing apparatus since the displacement between two solutions can be repeated many times without interfering with the experiments. It also increases the accuracy of the measurement by producing sharper start and end transitions for the current–time plot of the solution displacement process. In this design, efforts have been made to minimize the effects of electrolysis, Joule heating and undesired pressure driven flow on the measurements. An improvement on the current–time slope analysis is also presented. The Y-channel design was validated by comparing zeta potential measurements to published results. The zeta potential of several biological buffers relevant to the microfluidic community in plasma treated PDMS/PDMS and PDMS/Glass microchannels are presented. Preliminary studies of surface conductivity measurements using the Y-channel design were also conducted and are briefly discussed.     

Photo Noise

Like past avant-garde movements, glitch and noise in the twenty-first century are used to reveal the materiality of the medium. But instead of providing insight or clarity into the politics or social conditions of a technology, they demarcate broader conditions of opacity and blockage, contributing to what Jodi Dean has called ‘communicative capitalism’. The work of German photographer Thomas Ruff alongside the Düsseldorf School, New Photography and JPEG technology are used in this article to illustrate this tendency as a move away from meaning and toward visual noise and communicative breakdown. I use three core analytic registers: the deliberate failure to adhere to visual norms, communicative failure and machine failure and breakdown. Ruff’s work uses visual noise as art to at once reaffirm pervasive conditions of breakdown, failure and decline in high-tech global capitalism, while simultaneously providing a temporary reprieve from these conditions through an emergent form of visual beauty.     

Visualization and Analysis‐Oriented Reconstruction of Material Interfaces

Reconstructing boundaries along material interfaces from volume fractions is a difficult problem, especially because the under‐resolved nature of the input data allows for many correct interpretations. Worse, algorithms widely accepted as appropriate for simulation are inappropriate for visualization. In this paper, we describe a new algorithm that is specifically intended for reconstructing material interfaces for visualization and analysis requirements. The algorithm performs well with respect to memory footprint and execution time, has desirable properties in various accuracy metrics, and also produces smooth surfaces with few artifacts, even when faced with more than two materials per cell.     

Force and tactile feedback in preloaded cantilever snap-fits under manual assembly

The purpose of this study was to investigate the tactile feedback of snap-fit fasteners when used in manual assembly. An important aspect of this assembly process is the assembler’s ability to perceive the snap-fit’s engagement. This sensing of engagement yields a high level of confidence that assembly is both complete and secure. Force and tactile feedback are critical elements in this process. Many snap-fits are used in conjunction with sealing elements that produce a constant force that remains in effect after snap-fit assembly is completed. The effect of sealing element preload magnitude and stiffness was studied using a test station, force deflection measurements, and jury pool data. A low value of preload with low stiffness was determined to be most favorable in terms of force and tactile feedback, with no preload only slightly less favorable. In order to sense the engagement signal of the catch, some resistance to assembly was found to be beneficial. A dimensionless term called “engagement signal-to-hold-force ratio” is proposed as an additional way of rating the effect of assembly forces for snap-fits. It was found that higher signal-to-hold-force ratio, as well as higher values of engagement signal, corresponded to higher confidence of assembly among experimental subjects.

Relevance to Industry

In the automotive industry, force and tactile feedback are essential for sensing the full engagement of snap-fit parts during the assembly of critical components, such as electrical and fuel system interconnects. Both the design of the snap-fit and the presence or absence of preload will affect force and tactile feedback. Many of these applications require the compression of an elastic gasket with a preload needed for air, fuel, or electrical isolation. Preload and preload stiffness factors are examined in this paper with the aim of achieving a better understanding of these effects so that snap-fit confidence of assembly and assembly robustness can be enhanced in industrial settings.     

Next generation remote critical care through service-oriented architectures: challenges and opportunities

Health care providers and governments are under pressure to maintain and improve the quality of care to an increasing volume of critical care patients at either end of the life cycle, namely premature and ill term babies together with the elderly. The provision of a service of critical care utilizing real time service-oriented architectures has the potential to enable clinicians to be supported in the care of a greater number patients that are, perhaps more importantly, located elsewhere to their intensive care units. This paper presents a review of recent research in the application of computing and IT to support the service of critical care and determines the trends and challenges for the application of real time service-oriented architectures within the domain. It then presents some case study–based research on the design of a service-oriented architecture-based approach to support two aspects of critical care namely elderly care and neonatal intensive care to provide further context to trends and opportunities.     

Editorial

Higher-Order and Symbolic Computation -     

Sterol and steryl ester regulation of phospholipase A2 from the mosquito parasiteLagenidium giganteum

Lagenidium giganteum, a facultative parasite of mosquito larvae, cannot synthesize sterols, and requires an exogenous source of these lipids in order to enter its reproductive cycle. This parasite grows vegetatively in the absence of sterols, but requires cholesterol or structurally related compounds to produce motile zoospores, which are the only stage capable of infecting mosquitoes. Sterols structurally related to cholesterol and some steryl esters inhibited the activity ofL. giganteum phospholipase A₂ (PLA₂), an enzyme that hydrolyzes fatty acids from thesn-2 position of glycerophospholipids. Sterols that induce reproduction inhibitedL. giganteum PLA₂ activity, while sterols and steroids that do not support sporulation had minimal effect. Most steryl esters had no effect on enzyme activity, but cholesteryl arachidonate (CA) was a potent inhibitor of parasite PLA₂. Not all enzymes partly purified using a DEAE-Sephacel column were affected by these lipids, demonstrating selective inhibition of specific enzymes. Potency was enhanced by up to several orders of magnitude if epoxy fatty acids were esterified to the cholesterol nucleus. The steryl ester pool was dynamic during morphogenesis, and the fatty acid composition of the steryl esters did not mimic total cell or membrane (glycerophospholipid) fatty acid composition asL. giganteum proceeded through its growth cycle. Synthesis of CA and monoepoxy CA by the parasite was confirmed using electrospray mass spectrometry and collision-induced dissociation. Steryl derivatives selectively inhibited PLA₂ enzymes from bovine pancreas, snake venom, and human cytoplasmic 85-kDa PLA₂.     

Editorial

Higher-Order and Symbolic Computation -     

The question of organizational forensic policy

The objectives of an organization in combination with the formal policy together underpin the strategic direction that any organization will take. We all know that security begins with policy – in other words the rules of play. If policy is sound then the appropriate security measures can be implemented to protect the activities required to achieve the stated objectives as well as maintain the information assurance requirements – availability, integrity, authentication, confidentiality and non-repudiation.