Size-dependent bioavailability of hematite (α-Fe2O3) nanoparticles to a common aerobic bacterium

The size-dependent bioavailability of hematite (α-Fe(2)O(3)) nanoparticles to obligate aerobic Pseudomonas mendocina bacteria was examined using the natural siderophore-producing wild type strain and a siderophore(-) mutant strain. Results showed that Fe from hematite less than a few tens of nm in size appears to be considerably more bioavailable than Fe associated with larger particles. This increased bioavailability is related to the total available particle surface area, and depends in part on greater accessibility of the Fe to the chelating siderophore(s). Greater bioavailability is also related to mechanism(s) that depend on cell/nanomineral proximity, but not on siderophores. Siderophore(-) bacteria readily acquire Fe from particles <10 nm but must be in direct physical proximity to the nanomineral; the bacteria neither produce a diffusible Fe-mobilizing agent nor accumulate a reservoir of dissolved Fe in supernatant solutions. Particles <10 nm appear to be capable of penetrating the outer cell wall, offering at least one possible pathway for Fe acquisition. Other cell-surface-associated molecules and/or processes could also be important, including a cell-wall associated reducing capability. The increased bioavailability of <10 nm particles has implications for both biogeochemical Fe cycling and applications involving engineered nanoparticles, and raises new questions regarding biogenic influences on adsorbed contaminants.     

Potential role for peroxisome proliferator-activated receptor gamma in regulating luteal lifespan in the rat

Peroxisome proliferator-activated receptor gamma (PPARgamma) has been shown to stimulate progesterone production by bovine luteal cells. We previously reported higher expression of PPARgamma in old compared with new luteal tissue in the rat. The following studies were conducted to determine the role of PPARgamma in rat corpora lutea (CL) and test the hypothesis that PPARgamma plays a role in the metabolism of progesterone and/or luteal lifespan. Ovaries were removed from naturally cycling rats throughout the estrous cycle, and pseudopregnant rats. mRNA for PPARgamma and P450 side-chain cleavage (SCC) was localized in luteal tissue by in situ hybridization, and protein corresponding to PPARgamma and macrophages identified by immunohistochemistry. Luteal tissue was cultured with agonists (ciglitazone, prostaglandin J2) or an antagonist (GW-9662) of PPARgamma. Progesterone was measured in media by RIA and levels of mRNA for 20alpha-hydroxysteriod dehydrogenase (HSD) and bcl-2 were measured in luteal tissue after culture by RT-PCR. An inverse relationship existed between the expression of mRNA for SCC and PPARgamma. There was no effect of PPARgamma agonists or the antagonist on luteal progesterone production in vitro, or levels of mRNA for 20alpha-HSD. PPARgamma protein was localized to the nuclei of luteal cells and did not correspond with the presence of macrophages. In new CL, ciglitazone decreased mRNA for bcl-2 on proestrus, estrus, and metestrus. Interestingly, GW-9662 also decreased mRNA for bcl-2 on proestrus and diestrus in old and new CL, and on metestrus in new CL. These data indicate that PPARgamma is not a major player in luteal progesterone production or metabolism but may be involved in regulating luteal lifespan.     

Developmental changes in the concentration and composition of flavonoids in skin of a red and a green apple cultivar

Flavonoids from the skin of Granny Smith, a green apple cultivar, and Splendour, a red apple cultivar, were quantified by high-performance liquid chromatography for two seasons (1989–1990 and 1990–1991). Both cultivars contained a similar composition and concentration of quercetin glycosides and proanthocyanidins. Splendour also synthesised cyanidin glycosides during ripening. Quercetin glycosides and proanthocyanidins were highest in the skin of very young fruit of Granny Smith and decreased by 50% during fruit development. In Splendour, concentrations of quercetin glycosides and proanthocyanidins in the skin decreased by 50% from early to mid-season but then increased during ripening. Cyanidin glycosides in Splendour increased to about 1 mg g^-1 fresh weight during ripening. There were significant differences between the two cultivars but not between years. Total amount of flavonoids increased throughout the season as fruit surface area increased. For Granny Smith there was an estimated net synthesis per apple of 0.16 mg day^-1 quercetin glycosides, 0.1 mg day^-1 proanthocyanidins and for Splendour a net synthesis per apple of 0.28 mg day^-1 quercetin glycosides, 0.21 mg day^-1 proanthocyanidins and during ripening 0.21 mg day^-1 cyanidin glycosides. Relative proportions of major quercetin glycosides and proanthocyanidins were stable during fruit development. For Splendour, however, cyanidin glycoside synthesis was accompanied by a corresponding increase in quercetin glycoside and proanthocyanidin synthesis. The data suggest a coordinate regulation of enzymes in the flavonoid biosynthetic pathway during fruit development.     

Nutraceutical therapies for degenerative joint diseases: a critical review

There is growing recognition of the importance of nutritional factors in the maintenance of bone and joint health, and that nutritional imbalance combined with endocrine abnormalities may be involved in the pathogenesis of osteoarthritis (OA) and osteochondritis dissecans (OCD). Despite this, dietary programs have played a secondary role in the management of these connective tissue disorders. Articular cartilage is critically dependent upon the regular provision of nutrients (glucose and amino acids), vitamins (particularly vitamin C), and essential trace elements (zinc, magnesium, and copper). Therefore, dietary supplementation programs and nutraceuticals used in conjunction with non-steroidal, anti-inflammatory drugs (NSAIDs) may offer significant benefits to patients with joint disorders, such as OA and OCD. This article examines the available clinical evidence for the efficacy of nutraceuticals, antioxidant vitamin C, polyphenols, essential fatty acids, and mineral cofactors in the treatment of OA and related joint disorders in humans and veterinary species. This article also attempts to clarify the current state of knowledge. It also highlights the need for additional targeted research to elucidate the changes in nutritional status and potential alterations to the expression of plasma membrane transport systems in synovial structures in pathophysiological states, so that current therapy and future treatments may be better focused.     

Sweetener Blend Optimization by Using Mixture Design Methodology and the Electronic Tongue

Utilizing more than one sweetener has been shown to be an effective way to substitute sucrose in food products. The objective of this study was to apply the augmented simplex‐centroid mixture design for the optimization of acceptable sweetener blends using coconut sugar, agave, and stevia. Sweetener blends were evaluated in aqueous solutions and gluten‐free granola bars by a trained panel and consumers (n = 60). Significant differences were found between sweetener mixtures in solutions by both panelists and consumers (P < 0.05). Taste profiles for the sweetener solutions were also generated using the electronic tongue. Most consumer and trained intensity ratings were highly correlated (R² ≥ 0.79) with the electronic tongue taste profile analysis. Granola bars were also found to be significantly different (P < 0.05), with consumers preferring coconut sugar mixtures. Using contour plots and desirability function analysis, an optimal sweetener combination was found for a granola bar formulation of 89.9% coconut sugar, 6.1% agave, and 4% stevia. These results indicate that a mixture design can be a reliable way to develop new sweetener blends for product development.     

Determination of flavour compounds in beer using stir‐bar sorptive extraction and solid‐phase microextraction

The analysis of volatile compounds in beer is important for quality control in the brewing industry. In this study, stir‐bar sorptive extraction (SBSE) and solid‐phase microextraction (SPME), two solvent‐less enrichment techniques, were applied in combination with gas chromatography flame ionization detection (GC/FID) for the determination of four flavour compounds (isoamyl acetate, ethyl hexanoate, benzaldehyde, myrcene) in beer. Limits of detection, linearity and repeatability of both methods were determined using standard ethanol solutions, while accuracy was determined by conducting recovery tests on commercial beer samples. Both methods were characterized by high linearity (r > 0.996) and repeatability (RSD = 1.76–10.66%). When both methods were compared, higher recoveries were obtained by SBSE, with limits of detection 1.8–2.8 times lower compared with SPME. In the analysis of commercial beer samples using both methods, SBSE analysis resulted in higher recoveries, therefore demonstrating promise for the analysis of beer volatiles. Copyright © 2015 The Institute of Brewing & Distilling     

Lessons learned from a woodstove changeout on the Nez Perce Reservation

A woodstove changeout program was conducted within 16 homes on the Nez Perce Reservation in Idaho to evaluate the effectiveness of a woodstove changeout in improving indoor air quality. PM_2.5 samples were collected within the common area (rooms where the stoves were located) of the homes both before and after the installation of cleaner burning EPA-certified stoves. During the pre- and post-changeout sampling, indoor PM_2.5 mass, Organic Carbon (OC), Elemental Carbon (EC), and chemical markers of woodsmoke (including levoglucosan) were measured.Sampling results from this study showed that indoor air quality was improved in 10 of the 16 homes following the woodstove changeout and educational training program. Five homes had increased indoor PM_2.5 concentrations following the changeout, while one home did not have final PM_2.5 results for comparison. The median pre-changeout PM_2.5 mass (as measured by TSI DustTraks) was 39.2 μg/m³, with a median post-changeout concentration of 19.0 μg/m³. This resulted in an overall 52% reduction in median indoor PM_2.5, a 36% reduction in mean indoor PM_2.5 and a 60% reduction in PM_2.5 spikes when the old stoves were replaced with EPA-certified stoves. Another significant finding of the project was that targeted education and outreach is a critical component of the overall success of the program. Effective messaging to homeowners on proper use of their new stove is a necessary task of a woodstove changeout.     

Human rights-based approach to tobacco control

The Framework Convention for Tobacco Control (FCTC) is currently the most potent tool for implementation of tobacco control laws across the globe. The FCTC is derivative from previously constructed international human rights conventions. These previous conventions have enforcement mechanisms, unlike the FCTC. However, the FCTC relies on state parties to report periodically on its implementation rather than on a continuous monitoring system. The Human Rights and Tobacco Control Network proposes that abiding by the principles of human rights delineated by international treaties, citizens across the globe can demand effective action for tobacco control. This paper explains the link between fundamental human rights and the right to tobacco control. Mechanisms are described to link the FCTC and its principles with human rights-based monitoring reports, which are provided to oversight committees for the other human rights conventions. The initial work of the Human Rights and Tobacco Control Network is summarised and considers the future directions for the human rights-based approach to tobacco control.     

Sulfate metabolism in mycobacteria

Pathogenic bacteria have developed numerous mechanisms to survive inside a hostile host environment. The human pathogen Mycobacterium tuberculosis (M. tb) is thought to control the human immune response with diverse biomolecules, including a variety of exotic lipids. One prevalent M. tb-specific sulfated metabolite, termed sulfolipid-1 (SL-1), has been correlated with virulence though its specific biological function is not known. Recent advances in our understanding of SL-1 biosynthesis will help elucidate the role of this curious metabolite in M. tb infection. Furthermore, the study of SL-1 has led to questions regarding the significance of sulfation in mycobacteria. Examples of sulfated metabolites as mediators of interactions between bacteria and plants suggest that sulfation is a key modulator of extracellular signaling between prokaryotes and eukaryotes. The discovery of novel sulfated metabolites in M. tb and related mycobacteria strengthens this hypothesis. Finally, mechanistic and structural data from sulfate-assimilation enzymes have revealed how M. tb controls the flux of sulfate in the cell. Mutants with defects in sulfate assimilation indicate that the fate of sulfur in M. tb is a critical survival determinant for the bacteria during infection and suggest novel targets for tuberculosis drug therapy.