Contribution of the rat's neocortex to ingestive control: I. Latent learning for the taste of sodium chloride.

Conducted 3 experiments with 13 decorticate and 12 control male Sprague-Dawley rats to investigate whether some permanent ingestive control deficit would be revealed in a latent learning paradigm for salt taste. The ability of Ss to associate how they obtained the taste of NaCl when Na-replete was assessed by examining barpresses during extinction when Na-depleted. Intact Ss exposed to 4–6 hrs of NaCl taste training retained the association after decortication; decorticate Ss exposed to the same training acquired the association. Decorticate Ss exposed to as little as 2 min of NaCl taste training demonstrated the ability to associate barpressing with NaCl by their resistance to extinction. This association was specific to NaCl training. It is concluded that subcortical structures are adequate for latent learning involving NaCl taste. Conversely, previous research has shown that the neocortex is required for the retention of taste-aversion learning for the same taste. (43 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Integrated Rail‐to‐Rail Low‐Voltage Low‐Power Enhanced DC‐Gain Fully Differential Operational Transconductance Amplifier

In this paper, we present an integrated rail‐to‐rail fully differential operational transconductance amplifier (OTA) working at low‐supply voltages (1.5 V) with reduced power consumption and showing high DC gain. An embedded adaptive biasing circuit makes it possible to obtain low stand‐by power dissipation (lower than 0.17 mW in the rail‐to‐rail version), while the high DC gain (over 78 dB) is ensured by positive feedback. The circuit, fabricated in a standard CMOS integrated technology (AMS 0.35 μm), presents a 37 V/μs slew‐rate for a capacitive load of 15 pF. Experimental results and high values of two quality factors, or figures of merit, show the validity of the proposed OTA, when compared with other OTA configurations.     

PVK/MWNT electrodeposited conjugated polymer network nanocomposite films

The facile preparation of poly (N-vinyl carbazole) (PVK) and multiwalled carbon nanotubes (MWNTs) solution and conjugated polymer network (CPN) nanocomposite film is described. The stable solutions of PVK/MWNT were prepared in mixed solvents by simple sonication method, which enabled successful deaggregation of the MWNTs with the polymer matrix. MWNT was most effectively dissolved in N-cyclohexyl-2-pyrrolidone (CHP) compared to other solvents like N-methyl pyrrolidone (NMP), dimethyl formamide, and dimethyl sulfoxide (DMSO). The composite solution was relatively stable for months with no observable precipitation of the MWNTs. Thermogravimmetric analysis (TGA) revealed the thermal stability of the nanocomposite while the differential scanning calorimetry (DSC) showed an increasing melting (T(m)) and glass transition (T(g)) temperatures as the fraction of the MWNTs in the nanocomposite was increased. Cyclic voltammetry (CV) allowed the electrodeposition of the nanocomposite film on indium tin oxide (ITO) substrates and subsequent cross-linking of the carbazole pendant group of the PVK to form CPN films. Ultraviolet-visible (UV-vis), fluorescence, and Fourier transform infrared (FTIR) confirmed film composition while atomic force microscopy (AFM) revealed its surface morphology. Four-point probe measurements revealed an increase in the electrical conductivity of the CPN nanocomposite film as the composition of the MWNTs was increased: 5.53 × 10(-4) (3% MWNTs), 0.53 (5%), and 1.79 S cm(-1) (7%). Finally, the interfacial charge transfer resistance and ion transport on the CPN nanocomposite film was analyzed by electrochemical impedance spectroscopy (EIS) with a measured real impedance value of ～48.10 Ω for the 97% PVK and 3% MWNT ratio of the CPN nanocomposite film.     

Overexpression of Ubiquitin-Conjugating Enzyme E2C Is Associated with Worsened Prognosis in Prostate Cancer

To evaluate the role of ubiquitin-conjugating enzyme E2C (UBE2C) in prostate cancer (PCa) progression and prognosis, the TCGA and our PCa tissue microarray cohort were included in the study. Weighted gene co-expression network analysis (WGCNA) and non-negative matrix factorization were used to cluster patients and to screen genes that play a vital role in PCa progression (hub gene). Immunohistochemistry staining was used to evaluate the protein level of UBE2C in prostatic tissues. Through WGCNA, we found a gene co-expression module (named the purple module) that is strongly associated with the Gleason score, pathologic T stage, and biochemical recurrent status. Genes in the purple module are enriched in cell cycle and P53 signaling and help us to cluster patients into two groups with distinctive biochemical recurrent survival rates and TP53 mutation statuses. Further analysis showed UBE2C served as a hub gene in the purple module. The expression of UBE2C in PCa was significantly higher than that in paracancerous tissues and was remarkably associated with pathologic grade, Gleason score, and prognosis in PCa patients. To conclude, UBE2C is a PCa-progress-related gene and a biomarker for PCa patients. Therapy targeting UBE2C may serve as a promising treatment of PCa in the future.     

Understanding Mechanisms Underlying Non-Alcoholic Fatty Liver Disease (NAFLD) in Mental Illness: Risperidone and Olanzapine Alter the Hepatic Proteomic Signature in Mice

Patients with severe mental illness have increased mortality, often linked to cardio-metabolic disease. Non-alcoholic fatty liver disease (NAFLD) incidence is higher in patients with schizophrenia and is exacerbated with antipsychotic treatment. NAFLD is associated with obesity and insulin resistance, both of which are induced by several antipsychotic medications. NAFLD is considered an independent risk factor for cardiovascular disease, the leading cause of death for patients with severe mental illness. Although the clinical literature clearly defines increased risk of NAFLD with antipsychotic therapy, the underlying mechanisms are not understood. Given the complexity of the disorder as well as the complex pharmacology associated with atypical antipsychotic (AA) medications, we chose to use a proteomic approach in healthy mice treated with a low dose of risperidone (RIS) or olanzapine (OLAN) for 28 days to determine effects on development of NAFLD and to identify pathways impacted by AA medications, while removing confounding intrinsic effects of mental illness. Both AA drugs caused development of steatosis in comparison with vehicle controls (p < 0.01) and affected multiple pathways relating to energy metabolism, NAFLD, and immune function. AA-associated alteration in autonomic function appears to be a unifying theme in the regulation of hepatic pathology.     

Biallelic Variants in KIF17 Associated with Microphthalmia and Coloboma Spectrum

Microphthalmia, anophthalmia, and coloboma (MAC) are a group of congenital eye anomalies that can affect one or both eyes. Patients can present one or a combination of these ocular abnormalities in the so called “MAC spectrum”. The KIF17 gene encodes the kinesin-like protein Kif17, a microtubule-based, ATP-dependent, motor protein that is pivotal for outer segment development and disc morphogenesis in different animal models, including mice and zebrafish. In this report, we describe a Sicilian family with two siblings affected with congenital coloboma, microphthalmia, and a mild delay of motor developmental milestones. Genomic DNA from the siblings and their unaffected parents was sequenced with a clinical exome that revealed compound heterozygous variants in the KIF17 gene ({"type":"entrez-nucleotide","attrs":{"text":"NM_020816.4","term_id":"1677537661","term_text":"NM_020816.4"}}NM_020816.4: c.1255C > T (p.Arg419Trp); c.2554C > T (p.Arg852Cys)) segregating with the MAC spectrum phenotype of the two affected siblings. Variants were inherited from the healthy mother and father, are present at a very low-frequency in genomic population databases, and are predicted to be deleterious in silico. Our report indicates the potential co-segregation of these biallelic KIF17 variants with microphthalmia and coloboma, highlighting a potential conserved role of this gene in eye development across different species.     

Antimicrobial applications of electroactive PVK-SWNT nanocomposites

The antibacterial properties of a nanocomposite containing an electroactive polymer, polyvinyl-N-carbazole (PVK) (97 wt %), and single-walled carbon nanotubes (SWNT) (3 wt %) was investigated as suspensions in water and as thin film coatings. The toxic effects of four different PVK-SWNT (97:3 wt %) nanocomposite concentrations (1, 0.5, 0.05, and 0.01 mg/mL) containing 0.03, 0.015, 0.0015, and 0.0003 mg/mL of SWNT, respectively, were determined for planktonic cells and biofilms of Escherichia coli (E. coli) and Bacillus subtilis (B. subtilis). The results showed that the nanocomposite PVK-SWNT had antibacterial activity on planktonic cells and biofilms at all concentration levels. Higher bacterial inactivation (94% for E. coli and 90% for B. subtilis) were achieved in planktonic cells at a PVK-SWNT concentration of 1 mg/mL. Atomic force microscopy (AFM) imaging showed significant reduction of biofilm growth on PVK-SWNT coated surfaces. This study established for the first time that the improved dispersion of SWNTs in aqueous solutions in the presence of PVK enhances the antimicrobial effects of SWNTs at very low concentrations. Furthermore, PVK-SWNT can be used as an effective thin film coating material to resist biofilm formation.     

Assessment of Stochastic Operation Optimization for Reservoirs of Contrasting Scales

Deriving optimal release policies for dams and corresponding reservoirs is crucial for the sustainable water resources management of a region as they directly control the distribution of water to several users. Mathematical optimization algorithms can help in finding efficient reservoir operating strategies taking into account complex system constraints and hydrologic uncertainty. The robustness of operation optimization models may be influenced by physical reservoir characteristics such as size and scale and the effectiveness of a model for a particular case study does not always guarantee the same level of success for another application. This research focused on assessing the applicability of an implicit stochastic optimization (ISO) procedure to derive rule curves for two different dams of contrasting reservoir scales in terms of physical and operational characteristics. The results demonstrated the feasibility of the proposed technique for both small- and large-scale systems in view of the lower vulnerability provided by the ISO-derived policies in contrast to operations carried out by the standard reservoir operating policy as well as the proximity of the ISO operations with those by perfect-forecast deterministic optimization. The ISO procedure also provided operating rules similar to, and even less vulnerable than, those derived by stochastic dynamic programming.