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561.
Wenjun Zhu Qian Chen Qiutong Jin Yu Chao Lele Sun Xiao Han Jun Xu Longlong Tian Jinglei Zhang Teng Liu Zhuang Liu 《Nano Research》2021,14(1):212-221
Ultrasound with deep penetration depth and high security could be adopted in sonodynamic therapy(SDT)by activating sonosensitizers to generate cytotoxic reactive oxygen species(ROS).Herein,two-dimensional(2D)coordination nanosheets composed of Zn2+and Tetrakis(4-carboxyphenyl)porphyrin(TCPP)are fabricated.While exhibiting greatly enhanced ultrasoundtriggered ROS generation useful for noninvasive SDT,such Zn-TCPP 2D nanosheets show high loading capacity of oligodeoxynudeotides such as cytosine—phosphorothioate-guanine(CpG),which is a potent toll like receptor 9(TLR9)agonist useful in activating immune responses.Highly effective SD T of primary tumors could release tumor-associated antigens,which working together with Zn-TCPP/CpG adjuvant nanosheets could function like whole-tumor-cell vaccines and trigger tumor-specific immune responses.Interestingly,ultrasound itself could strengthen anti-tumor immune responses by improving the tumor-infiltration of T cells and limiting regulatory T cells in the tumor microenvironment.Thus,SDT using Zn-TCPP/CpG nanosheets after destruction of primary tumors could induce potent antitumor immune responses to inhibit distant abscopal tumors without direct SD T treatment.Moreover,SDT with Zn-TCPP/CpG could trigger strong immunological memory effects to inhibit cancer recurrence after elimination of primary tumors.Therefore,the 2D coordination nanosheet may be a promising platform to deliver potent SDT-triggered immunotherapy for highly effective cancer treatment. 相似文献
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Linxuan Li Tingting Zhu Lele Huang Maozhi Ren 《International journal of molecular sciences》2022,23(13)
Target of rapamycin (TOR) is a serine/threonine protein kinase that plays a central regulating role in cell proliferation, growth, and metabolism, but little is known about the TOR signaling pathway in Chlorella sorokiniana. In this study, a Chlorella sorokiniana DP-1 strain was isolated and identified, and its nutritional compositions were analyzed. Based on homologous sequence analysis, the conserved CsTOR protein was found in the genome of Chlorella sorokiniana. In addition, the key components of TOR complex 1 (TORC1) were present, but the components of TORC2 (RICTOR and SIN1) were absent in Chlorella sorokiniana. Pharmacological assays showed that Chlorella sorokiniana DP-1 was insensitive to rapamycin, Torin1 and KU0063794, whereas AZD8055 could significantly inhibit the growth of Chlorella sorokiniana. RNA-seq analysis showed that CsTOR regulated various metabolic processes and signal transduction pathways in AZD8055-treated Chlorella sorokiniana DP-1. Most genes involved in photosynthesis and carbon fixation in Chlorella sorokiniana DP-1 were significantly downregulated under CsTOR inhibition, indicating that CsTOR positively regulated the photosynthesis in Chlorella sorokiniana. Furthermore, CsTOR controlled protein synthesis and degradation by positively regulating ribosome synthesis and negatively regulating autophagy. These observations suggested that CsTOR plays an important role in photosynthesis and cellular metabolism, and provide new insights into the function of CsTOR in Chlorella sorokiniana. 相似文献
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566.
以软枣猕猴桃、葛枣猕猴桃及狗枣猕猴桃茎作为材料,考察液料比、超声功率、超声时间及乙醇浓度对总生物碱得率的影响,并探究三种猕猴桃茎中总生物碱对灰葡萄孢菌、胶孢炭疽菌、链格孢、多主棒孢霉和丁香假单胞杆菌猕猴桃致病变种的抑制效果。结果表明,三种猕猴桃茎中总生物碱提取最佳工艺为乙醇体积分数为92%,液料比24 mL/g,300 W超声20 min,软枣猕猴桃、葛枣猕猴桃及狗枣猕猴桃茎中总生物碱平均得率分别为0.41、0.43和0.39 mg/g。软枣猕猴桃茎中总生物碱对丁香假单胞杆菌猕猴桃致病变种抑制效果明显,MIC为50 mg/L,葛枣猕猴桃及狗枣猕猴桃茎中总生物碱对丁香假单胞杆菌猕猴桃致病变种抑制效果不明显;对灰葡萄孢菌、胶孢炭疽菌、链格孢及多主棒孢霉无抑制效果。该研究初步证实三种猕猴桃茎中均含有生物碱类物质,且对细菌具有一定的抑菌活性,为后续三种猕猴桃总生物碱的深入研究提供了理论依据。 相似文献
567.
568.
Lele Liu Fan Wang Xiaohan Wang Yaping Zhu Huimin Qi 《Polymer Engineering and Science》2023,63(1):281-294
Four poly(benzoxazine-co-siloxane) oligomers (PBcSs) were successfully synthesized by improving the synthesis step and controlling the ratio of phenol, bisphenol A, 1,3-bis(3-aminopropyl)tetramethyldisiloxane, m-aminophenyl acetylene, and formaldehyde; the resulting compounds all exhibited good processing properties. Siloxane addition can significantly improve the toughness of the polybenzoxazines. Additionally, compared with the PBcSs containing only phenol structure, the introduction of bisphenol A has a decelerating effect on the polymerization process of the benzoxazine oligomer, which leads to an increase in the exothermic peak temperature. Simultaneously, the PBcS containing bisphenol A also exhibits a higher glass transition temperature and increased thermal stability, but reduced flexural strength. Conversely, compared to the benzoxazine oligomers that do not contain alkynyl groups, the introduction of the terminal alkynyl group can accelerate the polymerization process, decrease the exothermic peak temperature, significantly increase the glass transition temperature, and greatly improve the heat resistance; the 5% thermal decomposition temperature of cured product is increased by up to 101°C. Furthermore, under conditions that ensure high flexural strength, appropriate alkynyl content can also significantly increase the material's flexural modulus. 相似文献
569.
570.
Zijian Xiong Lele Sun He Yang Zhisheng Xiao Zheng Deng Quguang Li Chenya Wang Fengyun Shen Zhuang Liu 《Advanced functional materials》2023,33(7):2211423
Interleukin 2 (IL2) is the first approved immunotherapeutic agent in cancer treatment. However, high-dose IL2 administrated through intratumoral injection still spreads all over the body, causing serious systemic toxicity. Herein, an injectable nickel-alginate hydrogel microsphere (Ni-ALGMS) to allow effective loading of IL2 and its sustained release after intratumoral administration is reported. In this design, histidine (his)-tagged IL2 is assembled into the Ni-ALGMS via the coordination bonds between his-tag and Ni2+. After injecting IL2-loaded Ni-ALGMSs (IL2@Ni-ALGMSs) into the tumor, IL2 slowly releases over long periods, thereby avoiding the risk of cytokine storm happening in IL2 systemic administration. Applying such IL2@Ni-ALGMSs for tumor model treatment can significantly increase the tumor infiltration of T lymphocytes, and effectively inhibit tumor growth, especially in combination with immune checkpoint inhibitors. This study presents a novel IL2 sustained-releasing platform for tumor immunotherapy, which can also be conveniently applied in other cytokines-based immunotherapies. 相似文献