Paracellular calcium transport across Caco-2 and HT29 cell monolayers

Intestinal calcium absorption has been shown to include two processes, a saturable transcellular movement and a non-saturable paracellular pathway. The potential utility of cell monolayers for studying transepithelial intestinal calcium transport has already been demonstrated; however, simultaneous evaluation of the contribution of the saturable transcellular and of the non-saturable paracellular processes to the total transepithelial transport has not yet been attempted. The aim of this study was to investigate the contribution both of transcellular and paracellular transport processes to the total transepithelial calcium transport in two cell culture monolayers. Caco-2 cells and a clone derived from HT-29 cells (HT29-Cl.19A), two cell lines derived from colon adenocarcinomas which are known to be able to exhibit typical enterocytic differentiation, were used. Cell monolayers were grown on a permeable support and used after 15 days of culture when these cells express enterocytic differentiation and high transepithelial resistance. Isotopic transport rate measurements were performed in the absence of a chemical gradient. The paracellular route was evaluated using [3H]mannitol. Calcium and [3H]mannitol transport rates across cell monolayers were not significantly different. Augmentation of calcium uptake by 200 mM sorbitol did not significantly increase calcium or mannitol transepithelial transport; however, calcium accumulation in the cells was increased by about 200%. Modulation of the monolayer permeability by addition of 10 nM vasoactive intestinal polypeptide (VIP) or 0.5 mM carbachol treatment, which respectively increased and decreased the transepithelial resistance, consequently modified calcium and mannitol transport in a parallel manner. Our results show that Caco-2 and HT29-Cl.19A cell monolayers are good models for studying the calcium paracellular transport pathway.     

The effect of multiple time scales and subexponentiality in MPEGvideo streams on queueing behavior

Guided by the empirical observation that real-time MPEG video streams exhibit both multiple time scale and subexponential characteristics, we construct a video model that captures both of these characteristics and is amenable to queueing analysis. We investigate two fundamental approaches for extracting the model parameters: using sample path and second-order statistics-based methods. The model exhibits the following two canonical queueing behaviors. When strict stability conditions are satisfied, i.e., the conditional mean of each scene is smaller than the capacity of the server, precise modeling of the interscene dynamics (long-term dependency) is not essential for the accurate prediction of small to moderately large queue sizes. In this case, the queue length distribution is determined using quasistationary (perturbation theory) analysis. When weak stability conditions are satisfied, i.e., the conditional mean of at least one scene type is greater than the capacity of the server, the dominant effect for building a large queue size is the subexponential (long-tailed) scene length distribution. In this case, precise modeling of intrascene statistics is of secondary importance for predicting the large queueing behavior. A fluid model, whose arrival process is obtained from the video data by replacing scene statistics with their means, is shown to asymptotically converge to the exact queue distribution. Using the transition scenario of moving from one stability region to the other by a change in the value of the server capacity, we synthesize recent queueing theoretic advances and ad hoc results in video modeling, and unify a broad range of seemingly contradictory experimental observations found in the literature. As a word of caution for the widespread usage of second-order statistics modeling methods, we construct two processes with the same second-order statistics that produce distinctly different queueing behaviors     

Textural evolution in electrodeposits under the influence of adsorbed foreign species: Part I Textural evolution in iron electrodeposits affected by hydrogen co-deposition

Textural evolution in electrodeposits is strongly affected by foreign species in the electrolyte and also by the co-deposition of hydrogen. The effects of foreign species on textural development in electrodeposits were studied by employing a Monte Carlo model. This model is based on physical principles and incorporates parameters which are used to control electrodeposition processes. An attempt was made in the simulation to reflect the basic process of the deposit growth, and to identify the factors responsible for the texture formation and its variation with the deposition condition. It is assumed that the texture of the deposit results from the minimization of the free energy of the system. Based on this assumption, it was demonstrated that the surface-energy anisotropy played an important role in the formation of fibre texture. In particular, the model can be used to simulate textural evolution under the influence of adsorbed foreign species, because the surface-energy anisotropy is modified by the adsorbed foreign species. Part I of the paper presents results of our studies on iron electrodeposition with the emphasis on the effect of hydrogen adsorption on iron deposit texture. Relevant experimental results have also been presented to corroborate the simulation study. Part II of the paper presents simulation studies of textural evolution in copper deposits under the influence of adsorbed potassium and chloride ions. This revised version was published online in November 2006 with corrections to the Cover Date.     

Breath and blood ethanol following use of a cough-cold preparation

All leaders in health care today are charged with the responsibility of transforming present practices into new and different ones that are needed for the future. The structural side of transforming is ultimately easier than the human side. However, the most frequent failures come from not concentrating sufficiently on the behavioral side of the change. Structural and psychological change must occur simultaneously and embrace each other for best results.     

Object-oriented H.263 compatible video coding platform forconferencing applications

This paper describes a prototype video coding platform meant for the conception and testing of multimedia products such as next-generation videophones. The platform is largely based on ITU-T Recommendation H.263, with a number of additional object-oriented quality enhancement features which make it especially well suited for very low bit-rate coding of “head-and-shoulders” video material typical of real-time multimedia applications, video teleconferencing, and video telephony. These features consist of: (1) segmentation into objects of interest, (2) segmentation-based prefiltering, (3) model-assisted rate control, (4) adaptive vector quantization, and finally (5) segmentation-based postfiltering. In the spirit of Recommendation H.263, these enhancements are modular and can be selectively turned on or off, thereby enabling a wide variety of coding modes     

The impact of modeling short circuit branches in state estimation

The authors discuss the modeling of zero impedance branches (short circuits) in power system state estimation. The concept of state variable in power system state estimation is generalized, and an extended version of the numerical observability method is proposed to cope with complex situations involving multiple short circuit branches, as they occur in some station arrangements. The impact of short circuit exact representation on bad data identification is also discussed     

Inference and retrieval of facial images

Attempts have been made to extend SQL to work with multimedia databases. We are reserved on the representation ability of extended SQL to cope with the richness in content of multimedia data. In this paper we present an example of a multimedia database system, Computer Aided Facial Image Inference and Retrieval system (CAFIIR). The system stores and manages facial images and criminal records, providing necessary functions for crime identification. We would like to demonstrate some core techniques for multimedia database with CAFIIR system. Firstly, CAFIIR is a integrated system. Besides database management, there are image analysis, image composition, image aging, and report generation subsystems, providing means for problem solving. Secondly, the richness of multimedia data urges feature-based database for their management. CAFIIR is feature-based. A indexing mechanism,iconic index, has been proposed for indexing facial images using hierarchical self-organization neural network. The indexing method operates on complex feature measures and provides means for visual navigation. Thirdly, special retrieval methods for facial images have been developed, including visual browsing, similarity retrieval, free text retrieval and fuzzy retrieval.     

Hepatic and splenic sarcoidosis: ultrasound and MR imaging

Two polypeptide antigens with molecular sizes of 34,000 daltons (34 kDa) and 38 kDa were separated from heated cells of a human clinical treponeme strain G7201 and Treponema denticola ATCC 35404, respectively. The rabbit polyclonal antisera against these antigens were produced and examined for their immunological reactions with the two heated antigens or intact spirochetal cells. Immunoblot analysis showed that the 34-kDa protein was also detected in T. denticola ATCC 35404 and ATCC 33520, and the 38-kDa protein was detected only in the two ATCC strains. Immunoelectron microscopy using the two rabbit antisera and protein A-gold complexes demonstrated that the 38-kDa protein antigen was present on the axial flagella of two T. denticola strains, and that the 34-kDa protein was located in the axial flagella of the G7201 cell, but neither in axial flagella nor on outer envelopes of the two ATCC strains cells, suggesting that the native 34-kDa axial flagellar protein of the G7201 strain may be different from that of T. denticola in terms of immunological reactivity.