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Hua Liu Wendan Chen Yushu Li Lei Sun Yuhong Chai Haixia Chen Haochen Nie Conglin Huang 《International journal of molecular sciences》2022,23(18)
The rapid growth of the global population has resulted in a considerable increase in the demand for food crops. However, traditional crop breeding methods will not be able to satisfy the worldwide demand for food in the future. New gene-editing technologies, the most widely used of which is CRISPR/Cas9, may enable the rapid improvement of crop traits. Specifically, CRISPR/Cas9 genome-editing technology involves the use of a guide RNA and a Cas9 protein that can cleave the genome at specific loci. Due to its simplicity and efficiency, the CRISPR/Cas9 system has rapidly become the most widely used tool for editing animal and plant genomes. It is ideal for modifying the traits of many plants, including food crops, and for creating new germplasm materials. In this review, the development of the CRISPR/Cas9 system, the underlying mechanism, and examples of its use for editing genes in important crops are discussed. Furthermore, certain limitations of the CRISPR/Cas9 system and potential solutions are described. This article will provide researchers with important information regarding the use of CRISPR/Cas9 gene-editing technology for crop improvement, plant breeding, and gene functional analyses. 相似文献
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Cheng Zhang Kailu Zhang Zihan Chai Yanfeng Song Xianrong Wang Yifan Duan Min Zhang 《International journal of molecular sciences》2022,23(18)
Androdioecy is the crucial transition state in the evolutionary direction of hermaphroditism to dioecy, however, the molecular mechanisms underlying the formation of this sex system remain unclear. While popular in China for its ornamental and cultural value, Osmanthus fragrans has an extremely rare androdioecy breeding system, meaning that there are both male and hermaphroditic plants in a population. To unravel the mechanisms underlying the formation of androdioecy, we performed small RNA sequencing studies on male and hermaphroditic O. fragrans. A total of 334 miRNAs were identified, of which 59 were differentially expressed. Functional categorization revealed that the target genes of differentially expressed miRNAs were mainly involved in the biological processes of reproductive development and the hormone signal transduction pathway. We speculated that the miRNA160, miRNA167, miRNA393 and miRNA396 families may influence the sex differentiation in O. fragrans. Overall, our study is the first exploration of miRNAs in the growth and development process of O. fragrans, and is also the first study of androdioecious plants from the miRNA sequencing perspective. The analysis of miRNAs and target genes that may be involved in the sex differentiation process lay a foundation for the ultimate discovery of the androdioecious molecular mechanism in O. fragrans. 相似文献
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在基于机器视觉的轮廓测量系统中,由于摄像机的透镜透光率不均,成像中存在光学畸变,对测量精度造成影响。为了对光学畸变进行校正,论文首先建立畸变数学模型,然后使用系统测量正方形标定板得到存在畸变的轮廓数据,根据存在畸变的轮廓数据的几何特征和直线约束条件使用LM优化算法完成对畸变模型中参数的标定。通过实验多次标定同一套轮廓测量系统,将标定结果与已有方法进行比较。实验结果表明,相较于已有算法,该论文所提方法多次标定的结果标准差更小,说明该论文所提方法具有更好的鲁棒性。 相似文献
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