Continuous monitoring in sewer networks an approach for quantification of pollution loads from CSOs into surface water bodies.

Different approaches for quantification of pollution loads discharged from combined sewer networks into surface water bodies have been observed over the last few years and decades, but a large number of unresolved problems still remain. Many monitoring campaigns have been based on manual or automated spot sampling - with the long known limitations of this method such as sampling errors and errors due to sample conservation, transport and preparation. On the other hand, only recently have sensors became available which are suitable for continuous application in sewer networks. A large number of practical problems still have to be solved before continuous monitoring in sewer networks will be successful. Additionally, most of the applicable sensors are based on surrogate methods which results in a considerable effort for reference measurements for sensor calibration. Finally, it has to be considered that, depending on the sewer network topography, deposition and remobilisation of pollutants varies considerably, which limits the generality of monitoring results and, subsequently, their applicability as a base for the design of storm water tanks or combined sewer overflows (CSO). A monitoring station for continuous monitoring of load discharges from a CSO has been installed and operated for more than one year. The design and equipment of the measurement station, operational experiences and results are given in this paper.     

Fullerene Single Crystals: Structure and Electronic Properties

This work covers an advanced investigation of the single crystal growth process for fullerenic materials and the contribution of Raman and IR spectroscopy to the understanding of the fcc high temperature rotor phase and the low temperature sc ratchet phase of pristine C₆₀. In addition substantial research contributions to the understanding of alkali metal doped fullerenes are presented.

Alkali metal doped crystals are studied with particular attention to the line broadening in the superconducting A₃C₆₀ phase and to the phases with stoichiometry A₁C₆₀. For the former electron-phonon coupling constants could be determined for all fivefold degenerated gerade modes and a total coupling strenght of λ = 0.90 was found. For the latter the different experimental conditions for obtaining the phase separated intermediate state or alternatively the orthorhombic polymeric state are analysed. Vibrational spectra for the polymeric state obtained from phototransformation and from mono alkali metal doping are found to be characteristically different, mainly with respect to the intensity of the Raman or IR response. Finally, research results obtained from the single crystals by various other techniques like thermal expansion or low frequency elastic deformation are summarized.     

Collision-free object movement using vector fields

Presents a technique for automatically providing animation and collision avoidance in a general-purpose computer graphics system. The technique, which relies on an expanded notion of vector fields, allows users to set up and animate objects easily, then prevents objects from colliding as the animation proceeds. This technique automatically generates volume octree vector fields around objects in a scene. These vector fields affect object motion and animation, and also provide for automatic collision avoidance for arbitrary objects. Applications of collision avoidance in an animation system encompass any scene containing object movement above or around other objects     

Hiding and perspective taking in long-tailed macaques (Macaca fascicularis).

Seven long-tailed macaques (Macaca fascicularis) were trained by threes not to drink from a juice nipple as long as an experimenter was facing them. However, they were allowed to drink when the experimenter was standing with his or her back turned. During transfer tests, the monkeys had a choice between 2 juice nipples, one uncovered and the other hidden from the experimenter by a wooden screen, while the experimenter was facing them. We tested whether the monkeys would then prefer to drink behind the screen, thus demonstrating that they transferred knowledge acquired during training. Results did not yield a significant outcome, suggesting that the macaques did not transfer the observable "experimenter's visible open eyes" and that they did not take the experimenter's perspective. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The ultrastructure of chicken gizzard vinculin as visualized by high-resolution electron microscopy

OBJECTIVES: A myocardial calcium-independent PLA2 has been described that is activated during myocardial ischemia and this enzyme may modulate ATP-sensitive potassium channels (KATP). The aim of this study was to determine the effect of an inhibitor of this enzyme, a bromoenol lactone, in isolated globally ischemic rat hearts. METHODS: Isolated rat hearts were treated for 10 min with 0.3-6 microM bromoenol lactone and then subjected to 25 min ischemia and 30 min reperfusion. RESULTS: The bromoenol lactone significantly increased coronary flow in nonischemic myocardium, and slightly reduced cardiac function at 6 microM. During global ischemia, time to contracture was significantly increased from vehicle group values in the presence of the bromoenol lactone (EC50 = 1.2 microM). During reperfusion, a concentration-dependent increase in function and a reduction in LDH release were observed for the PLA2 inhibitor. The concentrations of the PLA2 inhibitor which were significantly cardioprotective, inhibited this enzyme in membrane fractions of rat myocardium (IC50 = 0.87 microM). The KATP blocker sodium 5-hydroxydecanoate (5-HD) inhibited the increase in time to contracture observed for the bromoenol lactone. During reperfusion, 5-HD abolished the protective effects of the bromoenol lactone on cardiac function and LDH release. Glyburide had similar effects on the cardioprotective activity of the bromoenol lactone, although it only partially abolished the LDH reducing effect of this agent. CONCLUSIONS: The bromoenol lactone protects ischemic myocardium at concentrations which also inhibit calcium-independent PLA2. This cardioprotection can be attenuated by blockers of KATP, suggesting a potential mechanism for modulation of myocardial KATP.     

Structure-based design of beta-lactamase inhibitors. 1. Synthesis and evaluation of bridged monobactams

Bridged monobactams are novel, potent, mechanism-based inhibitors of class C beta-lactamases, designed using X-ray crystal structures of the enzymes. They stabilize the acyl-enzyme intermediate by blocking access of water to the enzyme-inhibitor ester bond. Bridged monobactams are selective class C beta-lactamase inhibitors, with half-inhibition constants as low as 10 nM, and are less effective against class A and class B enzymes (half-inhibition constants > 100 microM) because of the different hydrolysis mechanisms in these classes of beta-lactamases. The stability of the acyl-enzyme complexes formed with class C beta-lactamases (half-lives up to 2 days were observed) enabled determination of their crystal structures. The conformation of the inhibitor moiety was close to that predicted by molecular modeling, confirming a simple reaction mechanism, unlike those of known beta-lactamase inhibitors such as clavulanic acid and penam sulfones, which involve secondary rearrangements. Synergy between the bridged monobactams and beta-lactamase-labile antibiotics could be observed when such combinations were tested against strains of Enterobacteriaceae that produce large amounts of class C beta-lactamases. The minimal inhibitory concentration of the antibiotic of more than 64 mg/L could be decreased to 0.25 mg/L in a 1:4 combination with the inhibitor.     

Purity testing of recombinant proteins by capillary electrophoresis

Purity testing of recombinant DNA (rDNA) proteins using slab gel electrophoresis in conjunction with scanning densitometry is time consuming and labor intensive and is difficult to reproduce because the dyes used for visualizing proteins do not bind in a stoichiometric fashion for all proteins. The present report describes a micellar capillary zone electrophoresis (MCZE) procedure that overcomes these difficulties. The MCZE method was evaluated to estimate protein purity of hydrophobic cytomegalovirus proteins, expressed E. coli, and highly glycosylated hepatitis C virus proteins, expressed in Chinese hamster ovary cells. The results obtained by the MCZE procedure correlated very well with the purity results quantitated by the conventional slab gel electrophoresis method using purified Coomassie Brilliant Blue dye to reduce anomalies. MCZE may serve as an alternative method for in-process and purity testing of rDNA proteins.     

Differential Effects of 8-OH-DPAT on Two Forms of Appetitive Pavlovian Conditioning in the Rat.

Rats were trained on an appetitive Pavlovian conditioning task in which the conditioned stimulus (CS) was either localized (a light in the food tray) or nonlocalized (an increase in the general level of illumination). The conditioned response (CR) of approaching the site of food delivery in the presence of the CS was monitored. Presession treatment with the 5-HT1A agonist 8-OH-DPAT (subcutaneous injections at a dose of 0.15 mg/kg) retarded acquisition of the CR, but only when the localized CS was used. The results confirm the general proposal that serotonergic processes are involved in learning. The selective effect of the drug is not to be explained in terms of its motor effects and is consistent with the specific suggestion that systemic administration of 8-OH-DPAT is especially effective in disrupting learning tasks mediated by hippocampal mechanisms. (PsycINFO Database Record (c) 2010 APA, all rights reserved)