Caenorhabditis elegans has two isozymic forms, CE-1 and CE-2, of fructose-1,6-bisphosphate aldolase which are encoded by different genes

Two distinct types of cDNAs for fructose-1,6-bisphosphate (FBP) aldolase, Ce-1 and Ce-2, have been isolated from nematode Caenorhabditis elegans, and the respective recombinant aldolase isozymes, CE-1 and CE-2, have been purified and characterized. The Ce-1 and Ce-2 are 1282 and 1248 bp in total length, respectively, and both have an open reading frame of 1098 bp, which encodes 366 amino acid residues. The entire amino acid sequences deduced from Ce-1 and Ce-2 show a high degree of identity to one another and to those of vertebrate and invertebrate aldolases. The highest sequence diversity was found in the carboxyl-terminal region that corresponds to one of the isozyme group-specific sequences of vertebrate aldolase isozymes that play a role in determining isozyme-specific functions. Southern blot analysis suggests that CE-1 and CE-2 are encoded by different genes. Concerning general or kinetic properties, CE-2 is quite different from CE-1. CE-1 exhibits unique characteristics which are not identical to any aldolase isozymes previously reported, whereas CE-2 is similar to vertebrate aldolase C. These results suggest that CE-2 might preserve the properties of a progenitor aldolase with a moderate preference for FBP over fructose 1-phosphate (F1P) as a substrate, whereas CE-1 evolved to act as an intrinsic enzyme that exhibits a much broader substrate specificity than dose CE-2.     

Real-time output voltage control method of quasi-ZCS seriesresonant HF-linked DC-AC converter

This paper proposes a real-time control method of a series resonant high-frequency linked DC-AC converter employing quasi-zero current switching (quasi-ZCS) and a feedback control method for sinusoidal output voltage. An approximate analysis of the converter is performed, and then simplified equations and an equivalent circuit similar to the conventional PWM inverters/inverters are obtained. A real-time feedback control of the converter is realized using the equivalent circuit without detecting HF link current. The usefulness of the proposed control algorithm is confirmed by experimental results     

Highly specific enzyme immunoassay for human chorionic gonadotropin

A highly specific enzyme immunoassay for determining hCG was established by using beta-D-galactosidase as label. In order to increase the specificity of the assay, an antiserum against whole hCG was purified on a column of hCG beta carboxyl-terminal peptide (residues 123-145) covalently linked to Sepharose 4B. The antibody (N101S) thus prepared showed a weak cross-reactivity with human LH in an assay using hCG-enzyme conjugate, but the slight cross-reactivity was virtually avoided when an hCG beta carboxyl-terminal peptide was used as a peptide in the enzyme conjugate. N101S antibody was compared with antiserum (B1B) directed against a carboxyl-terminal peptide (123-145). In hCG measurement N101S gave about 30 times higher sensitivity than B1B, although the former antibody was less sensitive to carboxyl-terminal peptides of hCG beta. The enzyme immunoassay using a combination of N101S antibody and a carboxyl-terminal peptide (130-145)-enzyme conjugate was able to detect as little as 0.25 mIU of hCG without the interference of LH. The performance and validity of this assay were comparable to those of conventional radioimmunoassay.     

Brain potentials after clicking a mouse: a new psychophysiological approach to human-computer interaction

As a first step in developing a new psychophysiological technique to assess mental workload in human-computer interaction (HCI), we recorded event-related brain potentials for visual stimuli triggered by voluntary mouse clicks. Twelve university students clicked a mouse button at their own pace. Each click triggered 1 of 3 alphabetic letters assigned to frequent standard, rare target, and rare nontarget stimuli. Counting target stimuli was required. Both rare stimuli elicited a P3 (P300) wave, the amplitude of which was larger when the stimuli were triggered by mouse clicks than when the same stimuli were presented automatically without mouse clicks. Postmotor potentials associated with clicking were small in amplitude (<2 microV) and did not temporally overlap with the P3. The findings suggest that the P3 can be recorded for a computer's response to the user's intentional action and may be used as a measure of perceptual-central processing resources allocated to the HCI task. Actual or potential applications of this research include the evaluation of the user's attentional state during HCI byrecording brain potentials in the "mouse click" or action-perception paradigm.     

Photocatalytic degradation of trichloroethylene in water using TiO2 pellets

A recirculating system of aqueous trichloroethylene (TCE) solutions through the packed bed reactor with TiO2 pellets has been developed in order to mineralize TCE without difficulties for filtration and recovery of catalyst. The TiO2 pellets prepared by sol gel method have photocatalytic activity similar to commercially available PC-101 and PC-102 in the powder form and to ST-B11 pellets. In batch experiments with TiO2 powders, Degussa P-25 is the most active photocatalyst, which indicates that specific surface area is not an important factor controlling the photocatalytic activity in aqueous solutions. The degradation rates of TCE in the recirculating system with TiO2 pellets decreased in the presence of H2O2, while were remarkably accelerated by adding S2O8(2-). The presence of S2O8(2-) ions more than 0.01 mol dm(-3) completely suppressed hole-electron recombination and mineralized 50 ppm TCE with the 2 h irradiation. In a reactor without TiO2 photocatalysts, TCE was photodegraded by SO4- radicals which produced by photodissociation of S2O8(2-). The degradation rates increased with increase of the initial S2O8(2-) concentration. However, TCE was not mineralized but converted to intermediates which were slowly degraded to Cl- by continuing the irradiation.     

Visualization of Bubble Nucleation in Boiling 3He

The heat transfer properties of ³He bubbles in the nucleate boiling state have been investigated in liquid ³He below 1.0 K by using the shadowgraph method. The temperature difference between the copper surface and liquid ³He temperature was also measured as a function of heat flux in steady state. The size and number of bubbles departing from the surface in a specific time were compared using photograph recorded by a high-speed video camera at various heat flux and liquid ³He temperature of 0.5, 0.7 and 1.0 K.     

Speech recognition in living rooms: Integrated speech enhancement and recognition system based on spatial,spectral and temporal modeling of sounds

Research on noise robust speech recognition has mainly focused on dealing with relatively stationary noise that may differ from the noise conditions in most living environments. In this paper, we introduce a recognition system that can recognize speech in the presence of multiple rapidly time-varying noise sources as found in a typical family living room. To deal with such severe noise conditions, our recognition system exploits all available information about speech and noise; that is spatial (directional), spectral and temporal information. This is realized with a model-based speech enhancement pre-processor, which consists of two complementary elements, a multi-channel speech–noise separation method that exploits spatial and spectral information, followed by a single channel enhancement algorithm that uses the long-term temporal characteristics of speech obtained from clean speech examples. Moreover, to compensate for any mismatch that may remain between the enhanced speech and the acoustic model, our system employs an adaptation technique that combines conventional maximum likelihood linear regression with the dynamic adaptive compensation of the variance of the Gaussians of the acoustic model. Our proposed system approaches human performance levels by greatly improving the audible quality of speech and substantially improving the keyword recognition accuracy.     

Cross-correlation measurement without mechanical delay scanningusing electronically controlled wavelength-tunable femtosecond solitonpulse

A novel cross-correlation measurement system without mechanical action has been demonstrated in which the delay time between the reference and signal pulse can be electronically scanned by utilising the soliton self-frequency shift and the chromatic dispersion in the optical fibre. In this system, the reference pulse is the ideal femtosecond soliton pulse. The scanning range, the resolution and the scanning speed of the delay time can be arbitrarily changed only by the electronic control     

Two-dimensional theory of distributed feedback semiconductor lasers

A theory which takes account of the two dimensional waveguide structure of the distributed-feedback (DFB) laser is presented. Laser threshold conditions in the case of no external reflections are calculated for a three-layer model in which one cladding layer has a periodically changing dielectric constant. In contrast with the coupled-wave theory the threshold conditions are found to be asymmetric with respect to the Bragg frequency. The longitudinal mode which lies below and nearest the Bragg frequency has the lowest threshold gain. The difference between the threshold gains of the two adjacent longitudinal modes straddling the Bragg frequency has a maximum as a function of the coupled strength of the grating