Secretome analysis of novel IgE-binding proteins from Penicillium citrinum

The Penicillium genus of fungi is a frequently reported cause of allergic reactions. However, only a limited number of allergens have been reported. In Penicillium spp., many allergens show higher IgE-binding activity in culture filtrate extracts than in cellular extracts. In order to investigate the IgE-reactive profile of mold-sensitized patients, secreted IgE-reactive proteins from Penicillium citrinum were identified by 2-DE, serum immunoblotting, and nanoLC-MS/MS. Among the IgE-reactive spots, one known allergen, Pen c 13, and four novel allergens were identified. The cDNAs coding for Pen c 32 and Pen c 30 were cloned using designed primers based on nanoLC-MS/MS analysis. The amino acid sequences of Pen c 32 and Pen c 30 were, respectively, found to have extensive similarity with those of pectate lyases and catalases from various fungi. Native Pen c 30 was shown to have catalase activity and to bind to serum IgE from 48% of mold-allergic patients and induced immediate type skin reactions in a sensitized patient. Here, we present a proteome approach which resulted in the identification of four novel secreted allergens. These novel allergens might be useful in allergy diagnosis and in the treatment of mold-allergic disorders.     

Stream segregation algorithm for pattern matching in polyphonic music databases

As music can be represented symbolically, most of the existing methods extend some string matching algorithms to retrieve musical patterns in a music database. However, not all retrieved patterns are perceptually significant because some of them are, in fact, inaudible. Music is perceived in groupings of musical notes called streams. The process of grouping musical notes into streams is called stream segregation. Stream-crossing musical patterns are perceptually insignificant and should be pruned from the retrieval results. This can be done if all musical notes in a music database are segregated into streams and musical patterns are retrieved from the streams. Findings in auditory psychology are utilized in this paper, in which stream segregation is modelled as a clustering process and an adapted single-link clustering algorithm is proposed. Supported by experiments on real music data, streams are identified by the proposed algorithm with considerable accuracy.

Genetic Parallel Programming: design and implementation

This paper presents a novel Genetic Parallel Programming (GPP) paradigm for evolving parallel programs running on a Multi-Arithmetic-Logic-Unit (Multi-ALU) Processor (MAP). The MAP is a Multiple Instruction-streams, Multiple Data-streams (MIMD), general-purpose register machine that can be implemented on modern Very Large-Scale Integrated Circuits (VLSIs) in order to evaluate genetic programs at high speed. For human programmers, writing parallel programs is more difficult than writing sequential programs. However, experimental results show that GPP evolves parallel programs with less computational effort than that of their sequential counterparts. It creates a new approach to evolving a feasible problem solution in parallel program form and then serializes it into a sequential program if required. The effectiveness and efficiency of GPP are investigated using a suite of 14 well-studied benchmark problems. Experimental results show that GPP speeds up evolution substantially.     

压铸模标准模架参数化设计

采用AutoCAD二次开发语言－AutoLISP,在AutoCADR14环境下,开发了压铸模标准图形库系统。该系统包括对压铸模的镶块、套板、导柱、导套以及对这些零件进行校核和自动生成图纸的参数化设计。     

A polynomial fuzzy neural network for modeling and control

This paper introduces a novel neurofuzzy system based on polynomial fuzzy neural network (PFNN) architecture. A PFNN consists of a set of if-then rules with appropriate membership functions (MFs) whose parameters are optimized via a hybrid genetic algorithm. A polynomial neural network is employed in the defuzzification scheme to improve output performance and to select appropriate rules. A performance criterion for model selection is defined to overcome the overfitting problem in the modeling procedure. For a performance assessment of the PFNN inference system, two well-known problems are employed for a comparison with other methods. The results of these comparisons show that the PFNN inference system out-performs the other methods and exhibits robustness characteristics. This work was presented in part at the Fourth International Symposium on Artificial Life and Robotics, Oita, Japan, January 19–22, 1999     

Fault-based test suite prioritization for specification-based testing

Context

Existing test suite prioritization techniques usually rely on code coverage information or historical execution data that serve as indicators for estimating the fault-detecting ability of test cases. Such indicators are primarily empirical in nature and not theoretically driven; hence, they do not necessarily provide sound estimates. Also, these techniques are not applicable when the source code is not available or when the software is tested for the first time.

Objective

We propose and develop the novel notion of fault-based prioritization of test cases which directly utilizes the theoretical knowledge of their fault-detecting ability and the relationships among the test cases and the faults in the prescribed fault model, based on which the test cases are generated.

Method

We demonstrate our approach of fault-based prioritization by applying it to the testing of the implementation of logical expressions against their specifications. We then validate our proposal by an empirical study that evaluates the effectiveness of prioritization techniques using two different metrics.

Results

A theoretically guided fault-based prioritization technique generally outperforms other techniques under study, as assessed by two different metrics. Our empirical results also show that the technique helps to reveal all target faults by executing only about 72% of the prioritized test suite, thereby reducing the effort required in testing.

Conclusions

The fault-based prioritization approach is not only applicable to the instance empirically validated in this paper, but should also be adaptable to other fault-based testing strategies. We also envisage new research directions to be opened up by our work.     

基于ZigBee技术的学生宿舍监测系统设计

学生宿舍就寝后谈话及防火管理问题一直是学生管理部门的一大难题,针对学生宿舍集中、监测点多两大特点,提出一个基于ZigBee技术的解决方案.硬件设计以CC2430为核心,分析并设计出声、光、烟雾检测电路;为实现安装方便灵活性,各节点采用了灵活多样的供电方式.在分析ZStack协议栈操作系统的基础上,在其中实现了信号采集程序;监控PC机软件采用VC++及SQL数据库技术进行设计.经测试,系统能有效地采集、存储各路传感器信号并及时报警,为学生宿舍管理提供了一种方便有效的新方法.