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11.
EH Eddes AM Masclee HG Gooszen M Fr?lich CB Lamers 《Canadian Metallurgical Quarterly》1997,174(4):387-392
Two mobilizable cloning vectors, designated pABW1 and pAWB2, were constructed basing on the E. coli vector pBGS18 and oriT originating from RK2. In pABW2 the kanamycin resistance gene was replaced by a novel tetracycline resistance cassette derived from Tn1721. Both vectors, specific for E. coli, allow to perform the cloning steps in E. coli and then to efficiently transfer the constructs by conjugation to the host of choice. A vector which cannot propagate in the given host can be applied for identification of the host specific plasmid replicator regions. With the use of pABW2 we defined the minimal replicator region of pTAV202-a mini-derivative of the large pTAV1 plasmid of P. versutus. We also proved that RepC' encoded on this fragment is the principal initiator replication protein and that oriV is located along its coding sequence. 相似文献
12.
I Frías MT Caldeira JR Pérez-Casti?eira JP Navarro-Avi?ó FA Culia?ez-Maciá O Kuppinger H Stransky M Pagés A Hager R Serrano 《Canadian Metallurgical Quarterly》1996,8(9):1533-1544
The plasma membrane (PM) H(+)-ATPase has been proposed to play important transport and regulatory roles in plant physiology, including its participation in auxin-induced acidification in coleoptile segments. This enzyme is encoded by a family of genes differing in tissue distribution, regulation, and expression level. A major expressed isoform of the maize PM H(+)-ATPase (MHA2) has been characterized. RNA gel blot analysis indicated that MHA2 is expressed in all maize organs, with highest levels being in the roots. In situ hybridization of sections from maize seedlings indicated enriched expression of MHA2 in stomatal guard cells, phloem cells, and root epidermal cells. MHA2 mRNA was induced threefold when nonvascular parts of the coleoptile segments were treated with auxin. This induction correlates with auxin-triggered proton extrusion by the same part of the segments. The PM H(+)-ATPase in the vascular bundies does not contribute significantly to auxin-induced acidification, is not regulated by auxin, and masks the auxin effect in extracts of whole coleoptile segments. We conclude that auxin-induced acidification in coleoptile segments most often occurs in the nonvascular tissue and is mediated, at least in part, by increased levels of MHA2. 相似文献
13.
R. Hopfengärtner M. Lippert W. Dorsch H. Dietrich G. Kreiselmeyer G. Saemann-Ischenko 《Journal of Superconductivity and Novel Magnetism》1994,7(2):319-322
We report on measurements of the in-plane resistivityρ and Hall coefficientR H (B∥c) of various oxygen-deficient epitaxial films of YBa2Cu3O7?x in the normal state. The superconducting transition temperaturesT c of the samples vary from 14 to 90 K. Both the resistivity and the Hall coefficient exhibit a strong dependence on the oxygen content and the temperature. Asx increases,T c decreases continuously, whileρ andR H gradually increase in magnitude. Furthermore, also the characteristic linear dependences ofραT andR Hα T ?1 of the highly doped compounds changes to a nonlinear behavior for the samples withT c lower than 60 K. The unusual doping and temperature dependence ofR H will be compared to the predictions of our calculations, based on a two-dimensional tight-binding model using the relaxation-time approximation. The model considers also the next-nearest-neighbor hopping, which strongly influences the predicted Hall coefficient. Additionally, the cotangent of the Hall angle cot(Θ H ) is discussed in the framework of the two-dimensional Luttinger liquid theory. 相似文献
14.
15.
16.
In-Situ and Real-Time Investigation of Columnar-to-Equiaxed Transition in Metallic Alloy 总被引:1,自引:0,他引:1
H. Nguyen-Thi G. Reinhart N. Mangelinck-Noël H. Jung B. Billia T. Schenk J. Gastaldi J. Härtwig J. Baruchel 《Metallurgical and Materials Transactions A》2007,38(7):1458-1464
In this article, we present a review of observations during Al-3.5 wt pct Ni alloy solidi.cation experiments performed at
the European Synchrotron Radiation Facility (ESRF) in Grenoble. These experiments provide direct access to dynamical phenomena
during columnar growth (initial transient and breakdown of a planar solid-liquid interface), and for the first time to the
transition from columnar-to-equiaxed microstructure (nucleation ahead of a columnar front and blocking of a columnar front
by an equiaxed microstructure) and fully equiaxed growth (propagation of an effective front). Based on these experimental
observations, critical parameters such as columnar growth velocity variation during the transition or equiaxed-grain diameter
are measured and discussed.
This article is based on a presentation made in the symposium entitled “Solidi.cation Modeling and Microstructure Formation:
In Honor of Prof. John Hunt,” which occurred March 13–15, 2006, during the TMS Spring Meeting in San Antonio, Texas, under
the auspices of the TMS Materials Processing and Manufacturing Division, Solidification Committee. 相似文献
17.
F. -U. Gast P. S. Dittrich P. Schwille M. Weigel M. Mertig J. Opitz U. Queitsch S. Diez B. Lincoln F. Wottawah S. Schinkinger J. Guck J. Käs J. Smolinski K. Salchert C. Werner C. Duschl M. S. Jäger K. Uhlig P. Geggier S. Howitz 《Microfluidics and nanofluidics》2006,2(1):21-36
We describe a novel microfluidic perfusion system for high-resolution microscopes. Its modular design allows pre-coating of
the coverslip surface with reagents, biomolecules, or cells. A poly(dimethylsiloxane) (PDMS) layer is cast in a special molding
station, using masters made by photolithography and dry etching of silicon or by photoresist patterning on glass or silicon.
This channel system can be reused while the coverslip is exchanged between experiments. As normal fluidic connectors are used,
the link to external, computer-programmable syringe pumps is standardized and various fluidic channel networks can be used
in the same setup. The system can house hydrogel microvalves and microelectrodes close to the imaging area to control the
influx of reaction partners. We present a range of applications, including single-molecule analysis by fluorescence correlation
spectroscopy (FCS), manipulation of single molecules for nanostructuring by hydrodynamic flow fields or the action of motor
proteins, generation of concentration gradients, trapping and stretching of live cells using optical fibers precisely mounted
in the PDMS layer, and the integration of microelectrodes for actuation and sensing. 相似文献
18.
Kreppner Jana M.; Rutter Michael; Beckett Celia; Castle Jenny; Colvert Emma; Groothues Christine; Hawkins Amanda; O'Connor Thomas G.; Stevens Suzanne; Sonuga-Barke Edmund J. S. 《Canadian Metallurgical Quarterly》2007,43(4):931
Longitudinal analyses on normal versus impaired functioning across 7 domains were conducted in children who had experienced profound institutional deprivation up to the age of 42 months and were adopted from Romania into U.K. families. Comparisons were made with noninstitutionalized children adopted from Romania and with nondeprived within-U.K. adoptees placed before the age of 6 months. Specifically, the validity of the assessment, the degree of continuity and change in levels of functioning from 6 to 11 years, and the factors in the pre- and postadoption environment accounting for heterogeneity in outcome were examined. Pervasive impairment was significantly raised in children experiencing institutional deprivation for ≥6 months of life, with a minority within this group showing no impairment. There was no additional significant effect of duration of deprivation beyond the 6-month cutoff, and few other predictors explained outcome. The pattern of normality/impairment was mainly established by 6 years of age, with considerable continuity at the individual level between 6 and 11 years. The findings are discussed in terms of the possibility of a sensitive period for development. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
19.
J. Riikonen A. Säynätjoki M. Sopanen H. Lipsanen J. Ahopelto 《Journal of Materials Science: Materials in Electronics》2003,14(5-7):403-405
The growth of GaAs on polycrystalline silicon-on-insulator (SOI) substrates by metal organic vapor phase epitaxy using a two-step growth process was studied in this work. We have compared the variation of the growth temperature and the thickness of the initial GaAs epilayer in the structures grown on polycrystalline SOI to ones grown on SOI and epi-Si. Structural properties were studied using high-resolution X-ray diffraction (XRD). The growth temperature had a strong effect on the FWHM of the (0 0 4) XRD curve in structures grown on SOI and epi-Si. In the case of polycrystalline SOI the effect was not as strong. Optical properties of the samples in which the optically active layer consisted of self-organized In0.5Ga0.5As quantum dots were investigated by photoluminescence measurements. The photoluminescence intensity from structures grown on polycrystalline SOI was comparable to that from structures grown on SOI and epi-Si. 相似文献
20.