Sampling frequency and accuracy of SPM flux estimates in two contrasted drainage basins

The present paper is based on discharges and suspended particulate matter concentrations from a 9-years high-resolution database for the Garonne River (large plain river) covering contrasted hydrologic years, and a 12-months high frequency sampling for the Nivelle River (small mountainous river). Annual SPM fluxes in the Garonne River range from 0.6 x 10(6) t year(-1) (1997) to 3.9 x 10(6) t year(-1) (1996). In contrast, the Nivelle River transported 11 x 10(3) t year(-1) from December 1995 to December 1996. From the long-term observation of the Garonne River an empirical relation between SPM* (discharge-weighted mean annual SPM concentrations) and annual discharge was established. This relation allows estimating annual SPM fluxes for the Garonne River with less than 30% deviation from reference values for the whole range of mean annual discharge observed during the past decade. Specific (=area-normalized) annual SPM fluxes (YSPM) range from 11 to 74 t km(-2) year(-1) for the Garonne River. Comparison of these results with YSPM of the Nivelle River (69 t km(-2) year(-1) in 1996) suggests that interannual hydrological variations may have a greater impact on fluvial SPM transport than basin-specific parameters. By extracting individual SPM concentrations and corresponding discharge values from the database, different sampling frequencies were simulated and resulting SPM fluxes were then compared to reference fluxes derived from the complete database. If a deviation of simulated flux estimates from reference fluxes lower than +/-20% is accepted, the Garonne River (large plain river) must be sampled at least every 3 days (10 samples per month) and the Nivelle River every 7 h (approx. 100 samples per month). For the Garonne River this minimum sampling frequency is valid for all contrasted hydrologic years of the observation period. Below these minimum sampling frequencies, annual SPM flux estimates may greatly differ from reference fluxes (up to 200%) and there is high probability of systematic underestimation. Consequently, annual SPM flux estimates for the Garonne River derived from the empirical relation (SPM*-annual discharge) are likely to be more satisfactory (errors <30%) than estimates based on sampling frequencies lower than the minimum frequency. These findings underline the need of adapted sampling strategies for erosion assessment, reliable chemical (e.g. nutrients and pollutants) mass balances and characterisation of fluvial transport mechanisms in the world's contrasted watersheds.     

Ultrasound elastography based on multiscale estimations of regularized displacement fields

Elasticity imaging is based on the measurements of local tissue deformation. The approach to ultrasound elasticity imaging presented in this paper relies on the estimation of dense displacement fields by a coarse-to-fine minimization of an energy function that combines constraints of conservation of echo amplitude and displacement field continuity. The multiscale optimization scheme presents several characteristics aimed at improving and accelerating the convergence of the minimization process. This includes the nonregularized initialization at the coarsest resolution and the use of adaptive configuration spaces. Parameters of the energy model and optimization were adjusted using data obtained from a tissue-like phantom material. Elasticity images from normal in vivo breast tissue were subsequently obtained with these parameters. Introducing a smoothness constraint into motion field estimation helped solve ambiguities due to incoherent motion, leading to elastograms less degraded by decorrelation noise than the ones obtained from correlation-based techniques.     

The Gts1 protein stabilizes the autonomous oscillator in yeast

Continuous cultures of Saccharomyces cerevisiae show a robust autonomous temperature compensated oscillation in many metabolic functions. Respiratory activity, a convenient output to measure, oscillates with a period of 40 min. Deletion of GTS1, whose protein product has homology to the circadian per protein, has been implicated in temporal events within yeast, causes a reduction in periodicity to 18 min (wild-type period 40-60 min). The dilution rate was steadily increased from 0.04/h to 0.085/h and the oscillation stabilized after four to six dilutions. However, Gts1p's involvement in the maintenance and generation of metabolic synchrony, and in the central oscillating loop, appear to be minimal, as the mutant oscillation was robust and autonomous. Deletion of GTS1 did cause decreased temperature compensation of the period of the oscillation from Q(10) = 1.07 for the wild-type to Q(10) = 1.6 for the mutant. Also the degree of nutrient compensation observed for the wild-type was not observed in the GTS1-null mutant strain. It is postulated that Gts1p is involved in the mechanism that communicates external conditions, such as temperature, to the central oscillating loop.     

Estimation of demographic toxicity through the double bootstrap

Toxicity to organisms is usually expressed in terms of an observable effect on individuals from which a summary endpoint (such as the NOEC or ECx) is derived for risk assessment and environmental quality standards. However, toxicity evaluated in terms of a demographic endpoint may be more relevant to such regulatory applications. In this paper the effect of toxicity on population growth rate r is explored in tandem with a 'double bootstrap' to incorporate uncertainty. Exemplifying the approach with a set of individualized life table response data obtained for Daphnia magna exposed to zinc sulphate solution, the influence of increasing concentrations is assessed. A demographic-based metric for r, the ErCx (effect on r concentration percentage), is defined to permit alternative population level estimation of a 'safe effect' concentration.     

Growth and germination of proteolytic Clostridium botulinum in vegetable-based media

The growth of proteolytic Clostridium botulinum from spore inocula and changes in spore counts in mushroom, broccoli, and potato purées were monitored. Four strains of proteolytic C. botulinum types A and B were inoculated separately at approximately 10(4) spores per ml in nutrient broth and vegetable purées incubated at 15, 20, and 30 degrees C for up to 52 days. The times for the cell populations to increase 1,000-fold (T1,000) in the tested vegetables (1 to 5 days at 30 degrees C, 3 to 16 days at 20 degrees C, 7 to > 52 days at 15 degrees C) were similar to those for meat or fish. Only temperature significantly influenced growth rate. In contrast, the lag phase depended on the strains and media tested, in addition to temperature. Lag times and T1,000S for proteolytic C. botulinum were longer for potato and broccoli purées than for mushroom purée. These differences were not related to different pHs or redox potentials. The germination level, evaluated as the decrease in the spore count, was low. The addition of a germinant mixture (L-cysteine, L-alanine, and sodium lactate) to some strains inoculated in vegetable purées resulted in an increase in germination, suggesting a lack of germination-triggering agents in the vegetable purées.     

CRM in the European large-scale retail trade: Evidence and perspectives

This article sets forth the main findings of an empirical study carried out among the main actors in the sector of large-scale retailing in Europe, as regards their respective practical experience incrm (Customer Relationship Management) as well as the opportunities offered by the Internet technologies in this field. The evidence collected is checked against the problems expounded in the literature hinging on the following four main questions:

—the existence of national orientations that are taking shape in terms of the uses ofcrm

—the reality of the training processes of companies leading to e-crm as well as the acquisition of the technical and organisational maturity necessary to master these tools

—the variety of those uses and the issue of multichannelling

—the relative benefits of market relationship linked to the development ofcrm procedures

Thus this study offers an overview of emerging trends incrm peculiar to this sector.     

An examination of exposure control and content balancing restrictions on item selection in CATs using the partial credit model

The purpose of the present investigation was to systematically examine the effectiveness of the Sympson-Hetter technique and rotated content balancing relative to no exposure control and no content rotation conditions in a computerized adaptive testing system (CAT) based on the partial credit model. A series of simulated fixed and variable length CATs were run using two data sets generated to multiple content areas for three sizes of item pools. The 2 (exposure control) X 2 (content rotation) X 2 (test length) X 3 (item pool size) X 2 (data sets) yielded a total of 48 conditions. Results show that while both procedures can be used with no deleterious effect on measurement precision, the gains in exposure control, pool utilization, and item overlap appear quite modest. Difficulties involved with setting the exposure control parameters in small item pools make questionable the utility of the Sympson-Hetter technique with similar item pools.     

Cadmium and lead adsorption by a natural polysaccharide in MF membrane reactor: experimental analysis and modelling

In the present work, Pb(2+) and Cd(2+) adsorption onto a natural polysaccharide has been studied in membrane reactors. The process involves a stirred semi-batch reactor for the adsorption step and a microfiltration (MF) process in order to confine the particles. Due to their lower affinity for the biosorbent, Cd(2+) ions were found to breakthrough the process faster than Pb(2+) cations. The experimental results showed the technical feasibility of the pilot. A mass balance model based on the Langmuir equilibrium isotherm was used to describe the adsorption process. This relation is able to predict experimental data under different operating conditions: the adsorbent and metal concentrations, and the permeate flow rate. Based on these results, it is demonstrated that the biosorbent studied represents an interesting low-cost solution for the treatment of metal ions polluted waters.     

Standardized MR protocol for the evaluation of MRA sequences and/or contrast agents effects in high-degree arterial stenosis analysis

Purpose To investigate the relative role of high resolution (spatial or temporal) magnetic resonance angiography (MRA) sequence and of contrast agent properties in the evaluation of high-degree arterial stenosis. Methods We qualitatively and quantitatively studied both 50 and 95% (300 μm diameter) stenosis of a 6 mm arterial phantom with two contrast agents (CA), Gd-DOTA (r₁ =2.9 mM⁻¹ s⁻¹) versus P760 (r₁ =25 mM⁻¹ s⁻¹) at several CA concentrations, including arterial peak concentration after injection of either a single or double dose of CA, using either a high temporal (booster) or high spatial (HR) resolution 3D MRA sequences. Experimental data were then compared to theoretical data. Results With the 3D HR sequence, both visual and quantitative analysis were significantly better compared to the 3D booster sequence, at each phantom diameter. Quantitative analysis was significantly improved by injection of a double versus a single dose of each CA (Gd-DOTA or P760), primarily in high degree stenosis. Conclusion Combined MRA spatial resolution and high CA efficiency are mandatory to correctly evaluate high degree stenosis.     

Expression des émotions dans le dessin d'un homme chez l'enfant de 5 à 11 ans.

This study examines the development of children's ability to express emotions in their human figure drawing. Sixty children of 5, 8, and 11 years were asked to draw "a man," and then a "sad", "happy," "angry" and "surprised" man. Expressivity of the drawings was assessed by means of two procedures: a limited choice and a free labelling procedure. Emotionally expressive drawings were then evaluated in terms of the number and the type of graphic cues that were used to express emotion. It was found that children are able to depict happiness and sadness at 8, anger and surprise at 11. With age, children use increasingly numerous and complex graphic cues for each emotion (i.e., facial expression, body position, and contextual cues). Graphic cues for facial expression (e.g., concave mouth, curved eyebrows, wide opened eyes) share strong similarities with specific "action units" described by Ekman and Friesen (1978) in their Facial Action Coding System. Children's ability to depict emotion in their human figure drawing is discussed in relation to perceptual, conceptual, and graphic abilities. (PsycINFO Database Record (c) 2010 APA, all rights reserved)