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31.
Fluorescence lifetime imaging (FLIM) is a powerful microscopy technique for providing contrast of biological and other systems by differences in molecular species or their environments. However, the cost of equipment and the complexity of data analysis have limited the application of FLIM. We present a mathematical model and physical implementation for a low cost digital frequency domain FLIM (DFD-FLIM) system, which can provide lifetime resolution with quality comparable to time-correlated single photon counting methods. Our implementation provides data natively in the form of phasors. On the basis of the mathematical model, we present an error analysis that shows the precise parameters for maximizing the quality of lifetime acquisition, as well as data to support this conclusion. The hardware and software of the proposed DFD-FLIM method simplifies the process of data acquisition for FLIM, presents a new interface for data display and interpretation, and optimizes the accuracy of lifetime determination. 相似文献
32.
Recently, the use of lime mortars in the restoration of historic buildings has found a renewed interest because they can guarantee the required mechanical, chemical and physical compatibility with the existing substrate. Spontaneous occurrence of self-healing phenomena in lime-based mortars is well known; the possibility of engineering the self-healing capacity, through tailored additions, is therefore of the utmost interest with the aim of enhancing the durability of the building masonry restoration works. This work proposes a system for the evaluation of the self-healing capacity with reference to traditional and advanced lime mortars. The autogenous healing capacity of a reference lime mortar has been first of all evaluated. Then, the possibility of engineering the aforementioned capacity has also assessed, through both commercial crystalline admixtures and tailored encapsulated additives. These should work according to a twofold mechanism: first, the coated granules envelope a core of lime mortar with purpose of making it inert during the hardening phase. Secondly, once the coated granules rupture upon cracking and damage of the mortar, the reactive binder is released and undergoes a delayed hardening, which is responsible of the healing phenomena. The results show that the mortar is able to heal micro-cracks; moreover, the addition of the crystalline admixture enhances this capacity. The different kinds of employed coated granules were also able to induce a sensible self-healing, but they decrease the instantaneous compressive strength. 相似文献
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Jens Hauslage Medea Abbrecht Lars Hanke Ruth Hemmersbach Claudia Koch Wolfgang Hanke Florian P. M. Kohn 《Microgravity science and technology》2016,28(6):633-638
All life on earth has been established under conditions of stable gravity of 1g. Nevertheless, in numerous experiments the direct gravity dependence of biological processes has been shown on all levels of organization, from single molecules to humans. To study the effects especially of microgravity on biological systems, a variety of platforms are available, from drop towers to the ISS. Due to the costs of these platforms and their limited availability, as an alternative, numerous simulators have been developed for so called “simulated” microgravity. A classical systems is a clinostat, basically rotating a sample around one axis, and by integration of the gravity vector for 360° arguing that thus the effects of gravity are depleted. Indeed, a variety of studies has shown that taking out the direction of gravity from a biological system often results in consequences similar to the exposure of the system to real microgravity. Nevertheless, the opposite has been shown, too, and as a consequence the relevance of clinostats in microgravity research is still under discussion. To get some more insight into this problem we have constructed a small fluorescence clinostat and have studied the effects of clinorotation on the cytosolic calcium concentration of neuroglioma cells. The results have been compared to experiments with identical cells in real microgravity, utilizing parabolic flight missions. Our results show that in case of a cell suspension used in a small florescence clinostat within a tube diameter of 2mm, the effects of clinorotation are comparable to those under real microgravity, both showing a significant increase in intracellular calcium concentration. 相似文献
35.
Merle Schmahl Cecilia Müller Reinhard Meinke Erika G. Alves Alcantara Ude D. Hangen Claudia Fleck 《Advanced Engineering Materials》2023,25(10):2201676
Cyclic nanoindentation allows characterizing the influence of single phases and their interactions on fatigue mechanisms. Herein, a method for high cycle fatigue testing by nanoindentation is presented. By combining high- and low-frequency indentation modes, high cycle numbers are achieved while obtaining sufficient data points to reconstruct force–displacement hysteresis loops. A challenge is the stochastic course of thermal drift which is addressed by measuring drift rate in regular low-force holding segments. Drift rates are used to correct the displacement values, yielding reproducible cyclic deformation data as it is shown for two very different materials, a ductile metal and a brittle ceramic. 相似文献
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Claudia Archetti Dominique Feillet Alain Hertz M. Grazia Speranza 《Computers & Operations Research》2010,37(11):1860-1869
A profit and a demand are associated with each edge of a set of profitable edges of a given graph. A travel time is associated with each edge of the graph. A fleet of capacitated vehicles is given to serve the profitable edges. A maximum duration of the route of each vehicle is also given. The profit of an edge can be collected by one vehicle only that also serves the demand of the edge. The objective of this problem, which is called the undirected capacitated arc routing problem with profits (UCARPP), is to find a set of routes that satisfy the constraints on the duration of the route and on the capacity of the vehicle and maximize the total collected profit. We propose a branch-and-price algorithm and several heuristics. We can solve exactly instances with up to 97 profitable edges. The best heuristics find the optimal solution on most of instances where it is available. 相似文献
38.
Øyvind Hauge Claudia Ayala Reidar Conradi 《Information and Software Technology》2010,52(11):1133-1154
ContextOpen source software (OSS) is changing the way organizations develop, acquire, use, and commercialize software.ObjectiveThis paper seeks to identify how organizations adopt OSS, classify the literature according to these ways of adopting OSS, and with a focus on software development evaluate the research on adoption of OSS in organizations.MethodBased on the systematic literature review method we reviewed publications from 24 journals and seven conference and workshop proceedings, published between 1998 and 2008. From a population of 24,289 papers, we identified 112 papers that provide empirical evidence on how organizations actually adopt OSS.ResultsWe show that adopting OSS involves more than simply using OSS products. We moreover provide a classification framework consisting of six distinctly different ways in which organizations adopt OSS. This framework is used to illustrate some of the opportunities and challenges organizations meet when approaching OSS, to show that OSS can be adopted successfully in different ways, and to organize and review existing research. We find that existing research on OSS adoption does not sufficiently describe the context of the organizations studied, and it fails to benefit fully from related research fields. While existing research covers a large number of topics, it contains very few closely related studies. To aid this situation, we offer directions for future research.ConclusionThe implications of our findings are twofold. On the one hand, practitioners should embrace the many opportunities OSS offers, but consciously evaluate the consequences of adopting it in their own context. They may use our framework and the success stories provided by the literature in their own evaluations. On the other hand, researchers should align their work, and perform more empirical research on topics that are important to organizations. Our framework may be used to position this research and to describe the context of the organization they are studying. 相似文献
39.
Claudia Archetti Luca Bertazzi Giuseppe Paletta M. Grazia Speranza 《Computers & Operations Research》2011
We consider a production-distribution system, where a facility produces one commodity which is distributed to a set of retailers by a fleet of vehicles. Each retailer defines a maximum level of the inventory. The production policy, the retailers replenishment policies and the transportation policy have to be determined so as to minimize the total system cost. The overall cost is composed by fixed and variable production costs at the facility, inventory costs at both facility and retailers and routing costs. We study two different types of replenishment policies. The well-known order-up to level (OU) policy, where the quantity shipped to each retailer is such that the level of its inventory reaches the maximum level, and the maximum level (ML) policy, where the quantity shipped to each retailer is such that the inventory is not greater than the maximum level. We first show that when the transportation is outsourced, the problem with OU policy is NP-hard, whereas there exists a class of instances where the problem with ML policy can be solved in polynomial time. We also show the worst-case performance of the OU policy with respect to the more flexible ML policy. Then, we focus on the ML policy and the design of a hybrid heuristic. We also present an exact algorithm for the solution of the problem with one vehicle. Results of computational experiments carried out on small size instances show that the heuristic can produce high quality solutions in a very short amount of time. Results obtained on a large set of randomly generated problem instances are also shown, aimed at comparing the two policies. 相似文献
40.
Higher order sliding mode (HOSM) control design is considered for systems with a known permanent relative degree. In this paper, we introduce the robust Fuller's problem that is a robust generalization of the Fuller's problem, a standard optimal control problem for a chain of integrators with bounded control. By solving the robust Fuller's problem it is possible to obtain feedback laws that are HOSM algorithms of generic order and, in addition, provide optimal finite-time reaching of the sliding manifold. A common difficulty in the use of existing HOSM algorithms is the tuning of design parameters: our methodology proves useful for the tuning of HOSM controller parameters in order to assure desired performances and prevent instabilities. The convergence and stability properties of the proposed family of controllers are theoretically analyzed. Simulation evidence demonstrates their effectiveness. 相似文献