Geranylgeraniol and Neurological Impairment: Involvement of Apoptosis and Mitochondrial Morphology

Deregulation of the cholesterol pathway is an anomaly observed in human diseases, many of which have in common neurological involvement and unknown pathogenesis. In this study we have used Mevalonate Kinase Deficiency (MKD) as a disease-model in order to investigate the link between the deregulation of the mevalonate pathway and the consequent neurodegeneration. The blocking of the mevalonate pathway in a neuronal cell line (Daoy), using statins or mevalonate, induced an increase in the expression of the inflammasome gene (NLRP3) and programmed cell death related to mitochondrial dysfunction. The morphology of the mitochondria changed, clearly showing the damage induced by oxidative stress and the decreased membrane potential associated with the alterations of the mitochondrial function. The co-administration of geranylgeraniol (GGOH) reduced the inflammatory marker and the damage of the mitochondria, maintaining its shape and components. Our data allow us to speculate about the mechanism by which isoprenoids are able to rescue the inflammatory marker in neuronal cells, independently from the block of the mevalonate pathway, and about the fact that cell death is mitochondria-related.     

Preparation and characterization of light‐cured methacrylate/montmorillonite nanocomposites

Polymer/clay nanocomposites were prepared from dimethacrylate monomers, commonly used in dental restorative resins, and an organically modified silicate (montmorillonite). The photopolymerization process was hardly affected by the presence of the silicate filler, and thus 2 mm thick samples containing 3 wt% clay were extensively cured. Transmission electron microscopy revealed that the montmorillonite platelets were either intercalated or exfoliated. Nevertheless, for all formulations, intermediate‐sized aggregates of about 1 µm were present and their fraction increased as the amount of filler increased. The presence of the clay was found to have no major effect on the flexural modulus and compressive yield strength of the nanocomposites. Moreover, the water uptake of nanocomposites containing 3 wt% clay was about 10–15% higher than that of unfilled monomers. Modification of the clay surface with alternative organic cations is certainly necessary in order to achieve an optimal dispersion of the clay in the polymer matrix. Copyright © 2010 Society of Chemical Industry     

Gb3 Glycosphingolipids with Fluorescent Oligoene Fatty Acids: Synthesis and Phase Behavior in Model Membranes

Glycosphingolipids are involved in a number of physiological and pathophysiological processes, and they serve as receptors for a variety of bacterial toxins and viruses. To investigate their function in lipid membranes, fluorescently labeled glycosphingolipids are highly desirable. Herein, a synthetic route to access Gb₃ glycosphingolipids with fluorescently labeled fatty acids, consisting of pentaene and hexaene moieties either at the terminus or in the middle of the acyl chain, has been developed. The fluorescent properties of the Gb₃ derivatives were investigated in small unilamellar vesicles composed of a raft-like mixture. Phase-separated giant unilamellar vesicles (GUVs) allowed the quantification of the apparent partitioning coefficients of the Gb₃ compounds by means of confocal fluorescence laser scanning microscopy. The determined partition coefficients demonstrate that the Gb₃ derivatives are preferentially localized in the liquid-disordered (l_d) phase. To analyze whether the compounds behave like their physiological counterparts, Cy3-labeled (Cy: cyanine) Shiga toxin B subunits (STxB) were specifically bound to Gb₃-doped GUVs. However, the protein was favorably localized in the l_d phase, in contrast to results reported for STxB bound to naturally occurring Gb₃, which is discussed in terms of the packing density of the lipids in the liquid-ordered (l_o) phase.     

Cheaper membrane materials for microalgae dewatering

Among different strategies to reduce costs in microalgae dewatering process via cross-flow filtration, the one related to membrane material was investigated in order to be decreased. Several materials were tested, starting with the ones commonly used in membrane technology [ceramic, polysulfone (PSf) and polyacrylonitrile (PAN)] to the ones generally employed in packaging industry [acrylonitrile butadiene styrene (ABS), glycol-modified polyethylene terephthalate (PETG) and polylactic acid (PLA)], the latter being considerably cheaper. Experiments carried out showed promising results in terms of permeabilities for PSf–Pluronic^® F127 blended membranes and PAN membranes (11 ± 1 L/h/m²/bar and 22 ± 1 L/h/m²/bar, respectively, instead of 2 ± 2 L/h/m²/bar of PSf membranes), but with high related costs. PLA membranes showed good mechanical properties, biodegradability and price, but low permeability values (5 ± 1 L/h/m²/bar). PETG membranes showed attractive results in terms of costs and permeability, but poor mechanical properties. The polymer that offered the best results was the ABS that reached membrane permeabilities of 19 ± 1 L/h/m²/bar, maintaining good mechanical properties while filtering the microalgae Phaeodactylum tricornutum Bohlin. Thus, a novel functionality was found for these not so common polymers in microalgae dewatering. This indicates that use of these materials could also be considered in other aqueous micro/ultrafiltration applications. In addition, the biodegradable PLA polymer introduces a new concept of cheap and environmental friendly membrane in this application.     

Influence of the reaction time and the Triton x-100/Cyclohexane/Methanol/H2O ratio on the morphology and size of silica nanoparticles synthesized via sol–gel assisted by reverse micelle microemulsion

The present paper describes the synthesis of silica nanoparticles via the sol–gel method assisted by reverse micelle microemulsion, using reagents as Triton x-100/Cyclohexane/Methanol/H₂O, and also the effect on particle size of some synthesis parameters such as the water-surfactant molar ratio (R), Co-surfactant-surfactant (ρ), and synthesis time (t). The structure, morphology, and size of the silica nanoparticles were characterized with transmission electron microscopy and scanning electron microscopy. A variation of ρ = [Methanol]/[Triton X-100] affects the size, morphology, and dispersion of the particles. An increase in the concentration of methanol produces a decrease in particle size. The condition that resulted in smaller particle size, better spherical morphology, and monodispersity was when ρ = 7.6, which generated an approximate size of 83 ± 7 nm. The parameter R = [H₂O]/[Triton X-100] affects not only the size of the particles, but also their morphology. Higher values of R result in a decrease in the amount of catalyst present in the interior of the micelle, but in turn generate a greater amount of water, which results in a decrease in particle size and polydispersity. Time is a parameter that directly affects the size of the silica particles. The optimal time for the synthesis of nanoparticles was 2 h, resulting in silica nanoparticles of 25 ± 3 nm, monodisperse, with spherical morphology and without the presence of agglomerations.     

Effect of stocking density and group size on growth performance,carcass traits and meat quality of outdoor-reared rabbits

The effect of stocking density (16 rabbits/m², 5 rabbits/m², 2.5 rabbits/m², n = 60, Experiment 1) and group size (4 rabbits/cage, 8 rabbits/cage, 16 rabbits/cage, n = 88, Experiment 2) on productive performance, carcass and meat quality of a slow-growing rabbit population reared outdoors was investigated in two experiments. The highest stocking density induced the highest skin percentage. Lower stocking densities showed lower lightness of Biceps femoris and higher redness of Longissimus lumborum muscles. Four rabbits/cage group (Experiment 2) showed the highest daily weight gain and slaughter weight and the lowest skin percentage. The muscles of 16 rabbits/cage showed significantly higher pHu than 8 and 4 rabbits/cage. BF of 16 and 4 rabbits/cage showed higher L* value. Productive performance and meat quality of rabbits reared outdoors improved in low group size while stocking density needs more experiments. The best combination of density, group size and total available surface that showed the best production and carcass traits was of 5 rabbits/m², 4 rabbits/cage, and 0.8 m².     

Oxidation Kinetics of an Amorphous Silicon Carbonitride Ceramic

The oxidation kinetics of amorphous silicon carbonitride (SiCN) was measured at 1350°C in ambient air. Two types of specimens were studied: one in the form of thin disks, the other as a powder. Both specimens contained open nanoscale porosity. The disk specimens exhibited weight gain that saturated exponentially with time, analogous to the oxidation behavior of reaction-bonded Si₃N₄. The saturation value of the weight gain increased linearly with specimen volume, suggesting the nanoscale pore surfaces oxidized uniformly throughout the specimen. This interpretation was confirmed by high-resolution electron microscopy and secondary ion mass spectroscopy. Experiments with the powders (having a particle size much larger than the scale of the nanopores) were also consistent with measurements of the disks. However, the powder specimens, having a high surface-to-volume ratio, continued to show measurable weight gain due to oxidation of the exterior surface. The wide range of values for the surface-to-volume ratio, which included all specimens, permitted a separation of the rate of oxidation of the free surface and the oxidation of the internal surfaces of the nanopores. Surface oxidation data were used to obtain the rate constant for parabolic growth of the oxidation scale. The values for the rate constant obtained for SiCN lay at the lower end of the spectrum of oxidation rates reported in the literature for several Si₃N₄ and SiC materials. Convergence in the behavior of SiCN and CVD-SiC is ascribed to the purity of both materials. Conversely, it is proposed that the high rates of oxidation of sintered polycrystalline silicon carbides and nitrides, as well as the high degree of variability of these rates, might be related to the impurities introduced by the sintering aids.     

Attribute‐Domain Matrix: A Reverse Engineering Method for Innovation

An innovative idea is a beautiful thing, but most innovative ideas do not become successful new products. For products that are successful, there is a variety of examples over more than 150 years of situations where customers used them in ways never planned by the designer. Only few companies are systematically exploiting non‐conventional use as a method to generate ideas for new product outcomes and problem solutions. TRIZ awareness of analogies systematizes the search for available alternate situations: the attribute‐domain matrix actively transfers product attributes to unexpected application domains, breaks the functional fixedness pattern and helps make aware the resources that are available in any product or system.