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排序方式: 共有977条查询结果,搜索用时 15 毫秒
91.
Minc N Fütterer C Dorfman KD Bancaud A Gosse C Goubault C Viovy JL 《Analytical chemistry》2004,76(13):3770-3776
We present an experimental study of the microfluidic electrophoresis of long DNA in self-assembling matrixes of magnetic bead columns. Results are presented for the rapid separation of lambda-phage, 2lambda-DNA, and bacteriophage T4 DNA, where separation resolutions greater than 2 between lambda and T4 are achieved in times as short as 150 s. The use of a computer-piloted flow control system and injection results in high reproducibility between separations. We compare the experimentally measured mobility and dispersion with an exactly solvable lattice Monte Carlo model. The theory predicts that the mean velocity scales linearly with the field, the band broadening scales with the inverse of the field, and the resolution is independent of the field for intermediate fields-all of which are in accord with the experimental results. Moreover, reasonable quantitative agreement is achieved for band broadening for longer DNA (2lambda and T4) when the average postengagement time is measured experimentally. This work demonstrates the possibility of achieving fast microfluidic separation of large DNA on a routine basis. 相似文献
92.
Long‐term effects of angiotensin II blockade with irbesartan on inflammatory markers in hemodialysis patients: A randomized double blind placebo controlled trial (SAFIR study) 下载免费PDF全文
Christian D. Peters Krista D. Kjaergaard Claus H. Nielsen Kent L. Christensen Bo M. Bibby Jens D. Jensen Bente Jespersen 《Hemodialysis international. International Symposium on Home Hemodialysis》2017,21(1):47-62
Introduction: Low‐grade chronic inflammation is common in hemodialysis (HD) patients. Previous studies suggest an anti‐inflammatory effect of angiotensin II receptor blocker (ARB) treatment. The aim of this study was to compare the effect of ARB vs. placebo on plasma concentrations of inflammatory markers in HD patients. Methods: Adult HD patients were randomized for double‐blind treatment with the ARB irbesartan 150–300 mg/day or placebo. At baseline, 1 week, 3, 6, 9, and 12 months plasma high sensitivity C‐reactive protein (hsCRP), interleukin (IL)?1β, IL‐6, IL‐8, IL‐18, and transforming growth factor‐β (TGF‐β) were measured using Luminex and enzyme‐linked immunosorbent assay (ELISA) technology. Findings: Eighty‐two patients were randomized (placebo/ARB: 41/41). The groups did not differ in initial levels of any of the inflammatory markers (placebo/ARB median(range)): hsCRP 3.3(0.2–23.4)/2.7(0.2–29.6) μg/mL; IL‐1β 1.1(0.0–45.9)/1.1(0.0‐7.2) pg/mL; IL‐6 10(1–90)/12(1–84) pg/mL; IL‐8 31(9–134)/34(5–192) pg/mL; IL‐18 364(188–1343)/377(213–832) pg/mL; TGF‐β 3.2(0.8–13.9)/3.6(1.3–3.8) ng/mL. Overall, there was no significant difference in hsCRP, IL‐6, IL‐8, and TGF‐β between placebo and ARB‐treated patients during the study period, and hsCRP, IL‐6, IL‐8, and TGF‐β were relatively stable during the study period (P ≥ 0.18 in all tests for parallel curves, equal levels, and constant levels). The IL‐1β level was slightly different in the two groups over time, but not significantly (P = 0.09 in test for parallel curves) and it was also relatively stable during the study period (P ≥ 0.49 in tests for equal levels and constant level). IL‐18 was the only inflammatory marker which was not constant during the study period (P = 0.001 in test for constant level), but there was no significant difference between placebo and ARB‐treated (P ≥ 0.51 in tests for parallel curves and equal levels). Discussion: Inflammatory biomarkers were neither acutely, nor in the long‐term significantly affected by the ARB irbesartan. Our findings suggest that ARB treatment in HD patients does not offer protective anti‐inflammatory effects. 相似文献
93.
Closed-form solution for the Wigner phase-space distribution function for diffuse reflection and small-angle scattering in a random medium 总被引:1,自引:0,他引:1
Yura HT Thrane L Andersen PE 《Journal of the Optical Society of America. A, Optics, image science, and vision》2000,17(12):2464-2474
Within the paraxial approximation, a closed-form solution for the Wigner phase-space distribution function is derived for diffuse reflection and small-angle scattering in a random medium. This solution is based on the extended Huygens-Fresnel principle for the optical field, which is widely used in studies of wave propagation through random media. The results are general in that they apply to both an arbitrary small-angle volume scattering function, and arbitrary (real) ABCD optical systems. Furthermore, they are valid in both the single- and multiple-scattering regimes. Some general features of the Wigner phase-space distribution function are discussed, and analytic results are obtained for various types of scattering functions in the asymptotic limit s > 1, where s is the optical depth. In particular, explicit results are presented for optical coherence tomography (OCT) systems. On this basis, a novel way of creating OCT images based on measurements of the momentum width of the Wigner phase-space distribution is suggested, and the advantage over conventional OCT images is discussed. Because all previous published studies regarding the Wigner function are carried out in the transmission geometry, it is important to note that the extended Huygens-Fresnel principle and the ABCD matrix formalism may be used successfully to describe this geometry (within the paraxial approximation). Therefore for completeness we present in an appendix the general closed-form solution for the Wigner phase-space distribution function in ABCD paraxial optical systems for direct propagation through random media, and in a second appendix absorption effects are included. 相似文献
94.
Matthias Jger Claus Jordan Alwin Luttmann Wolfgang Laurig DOLLY Group 《International Journal of Industrial Ergonomics》2000,25(6):644-571
The load on the lumbar spine during occupational manual materials handling was previously investigated with respect to short activity sections or to specified load-handling types such as lifting or carrying. Within the so-called Dortmund Lumbar Load Study, analysis of the occupationally induced load on the lumbar spine during total working shifts in the field of surface construction, drop forge, industrial meat processing, and refuse collection was performed on the shop-floor. The body postures adopted, the action forces applied at the hands, and the resultant lumbar load for all load-handling tasks were analysed for 2 shifts in each field on the basis of video evaluations. Via a newly developed detailed classification procedure, the spatial position of the body segments as well as amplitude and direction of the action forces were described in a detailed manner. Consecutive biomechanical model calculations lead, for total shifts, to time courses of various measures for the load on the lumbar spine, such as flexion or torsional moments of force as well as compression and shear forces at the lumbosacral disc. In relation to recommended limits for the maximal disc compression provided in the literature, lumbar load is exceeded in numerous situations during a shift, in particular, with regard to persons of higher age. In a “dose model” applied in this study, the cumulative effect of single-task exposures was considered by superproportional weighting of the compressive force with respect to the corresponding duration of a working task.
Relevance to industry
A comprehensive evaluation of lumbar load for complete shifts is presented considering the real shop-floor conditions. Analyses for dustbin removal, surface construction and industrial meat processing have discovered numerous exceedings of lumbar-load limits. Such tasks should not be performed by older persons from the preventive point of view. 相似文献
95.
96.
Zusammenfassung Ziel dieses Artikels ist es, einen überblick über technische Sicherheitsl?sungen für Intranets zu geben. Ein Modell, das
die Anforderungen an ein Sicherheitssystem in Form von abstrakten Diensten abbildet, dient dazu, verschiedene L?sungsalternativen
zur Realisierung der unterschiedlichen Dienste auf Basis offener Standards oder generalisierter Produktklassen beurteilen
zu k?nnen. Darauf aufbauend wird eine beispielhafte Referenzarchitektur für Unternehmen auf Basis einer X.509 Public-Key-Infrastruktur
entworfen.
相似文献
97.
Supported gold catalysts, e.g., Au on Al2O3, Fe2O3, CeO2, MnO2, TiO2, ZrO2, activated carbon, titanium silicalite TS‐1, were prepared and used for the isomerization of linoleic acid (cis‐9,cis‐12‐octadecadienoic acid) to conjugated linoleic acids (CLA) in the presence of hydrogen at 165 °C in a batch reactor. The best results were obtained using a catalyst with 2 wt % Au on TS‐1, which exhibits a high selectivity (78 %) towards CLA. The two biologically active target CLA isomers, i.e., cis‐9,trans‐11‐CLA and trans‐10,cis‐12‐CLA, were the main products. During the isomerization of linoleic acid to CLA, consecutive reactions also took place. These were the hydrogenation of linoleic acid and CLA to monounsaturated octadecenoic acids and the further hydrogenation of monounsaturated acids to stearic acid. Thus, gold catalysts are capable of isomerizing linoleic acid to CLA and hydrogenating their double bonds to an extent that depends on the Au catalyst used. 相似文献
98.
Detlef Stiller Birgit Gaschler-Markefski Frank Baumgart Frank Schindler Claus Tempelmann Hans-Jochen Heinze henning Scheich 《Magma (New York, N.Y.)》1997,5(4):275-284
Prosodic modulation of speech provides information about emotional states of speakers (affective prosodies) or serves as syntactic
markers to change linguistic aspects of speech (linguistic prosodies). Previous electrophysiological investigations and studies
on patients with right or left hemisphere damage showed nonuniform results with respect to lateralization of prosodic processing.
In this study 20 healthy right-handed volunteers were investigated with functional magnetic resonance imaging of the acoustically
responsive areas on the supratemporal plane while detecting phonemes as control targets or prosodies in strings of nonsense
syllables and adjectives, the latter randomly intonated in a declarative, interrogative, commanding, happy, or sad fashion.
In control task A the phoneme /a/ was detected in the syllables. In control task B the phoneme /a/ was detected in the adjectives,
and in the experimental task C the sad intonations (affective) and in the experimental task D the interrogative intonations
(linguistic) had to be detected in the same material. In task A intensity-weighted volumes of activated voxels were not different
in the two hemispheres (laterality index 0). In task B with an irrelevant phoneme detection with respect to prosodic material,
the population split into two subgroups with similar right or left hemispheric lateralization of activity leading to an absolute
laterality index of 26.8 across all subjects. During detection of affective prosodies (task C), lateralization was maintained
yet the absolute laterality index reduced to 14.5, while there was no lateralization during detection of linguistic prosodies.
The sum of activations in the two hemispheres was the same across all tasks and subgroups, which suggests that the lateralizations
occurring with presentation and detection of prosodic material depend on a redistribution of activity between hemispheres. 相似文献
99.
Volker Claus 《Informatik-Spektrum》2005,28(2):124-128
Ein Ranking beruht meist auf einer Abbildung einer endlichen Menge von (hochkomplexen) Strukturen auf die reelle Achse. 相似文献
100.
Simplified counter-current distribution procedures are described which allow correct analysis of the α acids and iso α acids content of hop extracts. 相似文献