Measuring plant protein with the Bradford assay

The suitability of the Bradford protein assay for measuring plant protein was evaluated and a standard method developed. The assay involves extraction of dried, fresh, or frozen plant material in 0.1 NaOH for 30 min. Replicate 100-l aliquots of centrifuged supernatant are assayed with 5 ml Bio-Rad Bradford dye reagent (Coomassie brilliant blue G-250) diluted 1:4 and containing 3 mg/ml soluble polyvinylpyrollidone. Absorbance at 595 nm is recorded after 15 min against an NaOH blank. Samples are calibrated against a ribulose 1,5-diphosphate carboxylase-oxygenase standard in NaOH. Procedures for plant preparation, extraction stability, the effects of phenol removal and quinone formation, and assay recovery are evaluated. Assay absorbance stability and techniques for increasing absorbance stability are reported. Changes in protein quality are briefly discussed.     

Recent Progress in Applications of the Cold Sintering Process for Ceramic–Polymer Composites

Ceramic–polymer composites are of interest for designing enhanced and unique properties. However, the processing temperature windows of sintering ceramics are much higher than that of compaction, extrusion, or sintering of polymers, and thus traditionally there has been an inability to cosinter ceramic–polymer composites in a single step with high amounts of ceramics. The cold sintering process is a low‐temperature sintering technology recently developed for ceramics and ceramic‐based composites. A wide variety of ceramic materials have now been demonstrated to be densified under the cold sintering process and therefore can be all cosintered with polymers from room temperature to 300 °C. Here, the status, understanding, and application of cold cosintering, with different examples of ceramics and polymers, are discussed. One has to note that these types of cold sintering processes are yet new, and a full understanding will only emerge after more ceramic–polymer examples emerge and different research groups build upon these early observations. The general processing, property designs, and an outlook on cold sintering composites are outlined. Ultimately, the cold sintering process could open up a new multimaterial design space and impact the field of ceramic–polymer composites.     

Considering the possibility of bonding utilizing cold sintering for ceramic adhesives

Here, we introduce a new bonding technique that enables the joining of different materials at low temperatures and provides a bond superior to that of polymer adhesives at high temperatures, in temperature ranges between 250°C and 500°C. This technique involves a low temperature sintering process that is termed the “Cold Sintering Process,” where a dielectric composite powder material is sintered to function as the adhesive between two other materials being bonded. In order to characterize and further discuss the potential of this new bonding methodology, which we call Cold Sintering Ceramic Bonding (CSCB), we demonstrate the initial mechanical characteristics of samples with sandwich structures of mesh/CSCB/mesh, including four‐point bending, micro‐indentation, and adhesion pull tests. Where appropriate, we compare mechanical properties against low and high temperature epoxies and demonstrate that the CSCB matches up competitively with the epoxies at low temperatures and remains strong at temperatures well above those where standard polymer adhesives fail. Transmission electron microscopy show a high quality interface between a stainless steel plate and the ceramic after the CSCB.     

Wastewater treatment by radial freezing with stirring effects

Radial freezing experiments on wastewater models were conducted in the presence of imposed stirring in order to remove impurities. The studied samples (dilute Na-montmorillonite suspensions charged with nitrates and with zinc or lead) were placed inside a cylindrical annulus, cooled at a controlled temperature around -7 degrees C at its inner wall which rotated around a vertical axis. The freezing front propagated toward the still outer wall which was maintained at a constant temperature around +1 degrees C. Thanks to stirring, considerable purification rates up to 99.97% were attained. It was also demonstrated that combining radial freezing and stirring ended in residual concentrations which agreed with drinking water standards.     

Boston harbor outfall tunnel: An environmental imperative

In February 1989, the Massachusetts Water Resource Authority (MWRA) began the design and construction of a 15.3-km subsea tunnel and ocean riser system to discharge effluent in conjunction with the design and construction of a large secondary treatment plant. The court-mandated schedule required the tunnel and risers to be completed in July 1995. The paper discusses the project from the viewpoints of management, concept design and construction methods, including the ocean riser system. Key to th success of the project is the accuracy of the location of the risers. The design calls for a final tunnel alignment to be driven within 10 m of the riser shafts, beginning at a distance of 13.1 km from shore.     

Domestic dogs (Canis familiaris) use human gestures but not nonhuman tokens to find hidden food.

The authors examined the ability of domestic dogs to use human body cues (gestures) and equivalent-sized nonhuman cues to find hidden food in an object choice paradigm. In Experiment 1 the authors addressed the importance of the human element of the cue, and the effects of size, topography, and familiarity on dogs' success in using cues. Experiment 2 further explored the role of the human as cue-giver, and the impact of a change in the experimenter's attentional state during cue presentation. This included a systematic test of the role inanimate tokens play as cues apart from human placement. Our results indicate that dogs are more sensitive to human cues than equivalent nonhuman cues, and that the size of the cue is a critical element in determining dogs' success in following it. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

In situ analysis of living embryonic stem cells by coherent anti-stokes Raman microscopy

Embryonic stem cells (ESC), derived from preimplantation embryos, are defined by their ability to both self-renew and differentiate into all of the cells and tissues of a mature animal. Efforts to develop methods for in vitro culture of ESC for research or eventual therapeutic applications are hampered by the lack of rapid, nondestructive assays for distinguishing ESC from other (differentiated) cells within a growing culture. Coherent anti-Stokes Raman scattering (CARS) microscopy is shown here to be a sensitive and nondestructive method for identifying mouse ESC based on selective observation of specific molecular vibrations believed to be spectroscopic markers indicating the differentiated vs undifferentiated states of such cells. The nonlinear nature of CARS also permits imaging with subcellular resolution, potentially offering a means by which chemical changes accompanying the early stages of differentiation may be associated with certain intracellular compartments (e.g., nucleus, cytoplasm, membranes). A novel exposure/collection configuration is described, which yields high collection efficiency and low interference from nonresonant background components.     

Effects of sodium and potassium fertilisers on the composition of herbage and its acceptability to dairy cows

Fertilisation of herbage with Na can increase acceptability to cows, but the influence of fertiliser rate and fertilisation by K is unknown. In experiment 1, ten cows were grazed on pasture plots that had just been fertilised with 0–132 kg-Na ha⁻¹ (current Na) and had received 0–64 kg-Na ha⁻¹ in the previous grazing season (residual Na). Herbage Na concentration increased in proportion to current Na from 2·7 to 4·9 g-Na kg⁻¹ dry matter (DM) and also increased with increasing residual Na from 2·2 to 4·5 g-Na kg⁻¹ DM. Herbage K concentrations were low (10 g kg⁻¹ DM at 0 kg-Na ha⁻¹) and were only slightly reduced by Na fertiliser. Herbage Mg and Ca concentrations and DM digestibility were maximum at 66–99 kg-current-Na ha⁻¹. Cows grazed current-Na-fertilised plots to a lower height and spent more time grazing them. In experiment 2, pasture plots received no fertiliser, low and high isomolar and independent applications of Na and K or a combination of the two. The herbage was more mature than in experiment 1 and Na concentration of the herbage without Na fertilizer was high (5 g kg⁻¹ DM). Na fertiliser, therefore, only slightly increased Na concentration, more in clover than in grass, and had little effect on K concentration. K fertiliser increased K concentration from 16 to 20 g kg⁻¹ DM and reduced Na concentration to 3·5 g kg⁻¹ DM. Sodium fertiliser, therefore, only increased the acceptability of herbage to cattle when herbage Na concentrations were initially low (less than 5 g kg⁻¹ DM) and were increased substantially by the application of the fertiliser. © 1998 SCI.     

Inhibition of mutagenic PhIP formation by epigallocatechin gallate via scavenging of phenylacetaldehyde

Chemical model investigation showed that both epigallocatechin gallate (EGCG) and its peracetate, which has all the hydroxyl groups acetylated, effectively reduced the formation of 2‐amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine (PhIP), the most abundant mutagenic heterocyclic amine found in foods. Mechanistic study was subsequently carried out to characterize the probable inhibitory mechanism involved. GC‐MS analysis showed that EGCG in only one‐fourth molar quantity of phenylalanine reduced formation of phenylacetaldehyde, a key PhIP intermediate by nearly 90%. Its peracetate also showed similar inhibitory activity. This further supported the existence of an antioxidant‐independent mechanism contributing to the inhibition of PhIP formation by EGCG. Subsequent LC‐MS analyses of samples from a wide range of model systems consisting of PhIP precursors showed the generation of characteristic analytes with molecular weight corresponding to the sum of EGCG and phenylalanine fragment(s) only in models where phenylalanine and EGCG were simultaneously present. An isotope‐labeling study revealed that these analytes all contained fragment(s) of phenylalanine origin. Direct reaction employing phenylacetaldehyde and EGCG further confirmed the capability of EGCG to form adducts with phenylacetaldehyde, thus reducing its availability for PhIP formation. Finally, an investigation of the time course of the generation of postulated adduction products supported EGCG as an effective inhibitor of PhIP formation in prolonged heating processes.