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排序方式: 共有396条查询结果,搜索用时 15 毫秒
391.
The aim of this research was to identify the risk factors associated with the transfer of bacterial contamination from the fleece to the ovine carcass thereby providing the scientific basis for the development and validation of a clean sheep policy. Two hundred sheep in lairage were graded into five categories each consisting of 40 sheep. The categories were as follows; (A) clean and dry; (B) clean and wet; (C) dirty and dry; (D) dirty and wet and (E) visible dags (dung-clotted tufts of wool) categorized by the chief veterinary inspector at the slaughter plant based on the visual inspection of the hygienic status of the fleece. Microbiological evaluations of the carcasses were conducted using swab sampling methods. Total viable counts (TVCs), Enterobacteriaceae and coliform counts were obtained from 40 animals per category at four separate sites (brisket, shoulder, flank and rump) immediately after pelt removal. Statistical analysis of TVC data obtained from the carcass indicated that the dirt level of the fleece had a significant effect on contamination levels when the fleece was dry. Enterobacteriaceae and coliform counts suggest that dirt was a contributing risk factor regardless of wetness or dryness of the animal. The clean sheep policy should therefore differentiate between clean and dirty sheep and mandate additional hygiene measures for the latter. 相似文献
392.
Syrian hamster embryo (SHE) cells were investigated for their growth factor responsiveness as well as changes in growth factor homeostasis, including alterations in autocrine growth factor production and growth factor responsiveness, during in vitro transformation. For wild-type SHE cells, fetal bovine serum (FBS), epidermal growth factor (EGF) family members, platelet derived growth factor (PDGF) family members, fibroblast growth factor family members, interleukin-4, interleukin-9, oncostatin M, hepatocyte growth factor, erythropoietin and pituitary extract were found to be mitogenic. SHE cell mitogenesis was inhibited in response to transforming growth factor beta (TGF-beta) family members, interleukin-1 alpha, interleukin-1 beta and nerve growth factor. Additional experiments were conducted to study alterations in growth factor responsiveness to three SHE cell mitogens (FBS, EGF and PDGF) and one inhibitor of mitogenesis (TGF-beta) during SHE cell in vitro transformation. Alterations in either EGF, PDGF or TGF-beta responsiveness were observed in 7/8 SHE transformed lineages during the stepwise transformation process. Finally, 6/8 lineages underwent alterations which resulted in the production of autocrine growth factors during the transformation process. These results indicate that multiple alterations in growth factor homeostasis occur during the in vitro transformation process. 相似文献
393.
Cody W. Weyhrich John W. Will Garvit Nayyar Clarissa C. Westover Steven Patterson Clay B. Arrington Christopher B. Williams Timothy E. Long 《Small (Weinheim an der Bergstrasse, Germany)》2023,19(32):2303188
Vat photopolymerization (VP) Additive Manufacturing (AM), in which UV light is selectively applied to cure photo-active polymers into complex geometries with micron-scale resolution, has a limited selection of aliphatic thermoset materials that exhibit relatively poor thermal performance. Ring-opening dianhydrides with acrylate-containing nucleophiles yielded diacrylate ester-dicarboxylic acids that enabled photo-active polyimide (PI) precursors, termed polysalts, upon neutralization with an aromatic diamine in solution. In situ FTIR spectroscopy coupled with a solution and photo-rheological measurements revealed a previously unknown time-dependent instability of 4,4′-oxydianiline (ODA) polysalts due to an aza-Michael addition. Replacement of the electron-donating ether-containing diamine with an electron withdrawing sulfone-containing monomer, e.g., 4,4′-diaminodiphenyl sulfone (DDS), prohibited the aza-Michael addition of the aromatic amine to the activated acrylate double bond. Novel DDS polysalt photocurable solutions are similarly analyzed and validated long-term stability, which enabled reproducible printing of polyimide organogel intermediates. Subsequent VP AM afforded 3-dimensional (3D) structures of intricate complexity and excellent surface finish, as demonstrated with scanning electron microscopy. In addition, the novel PMDA-HEA/DDS solution enabled the production of the first beam latticed architecture comprised of all-aromatic polyimide. The versatility of a polysalt platform for multi-material printing is further demonstrated by printing parts with alternating polysalt compositions. 相似文献
394.
Design studies are an integral method of visualization research with hundreds of instances in the literature. Although taught as a theory, the practical implementation of design studies is often excluded from visualization pedagogy due to the lengthy time commitments associated with such studies. Recent research has addressed this challenge and developed an expedited design study framework, the Design Study “Lite” Methodology (DSLM), which can implement design studies with novice students within just 14 weeks. The framework was developed and evaluated based on five semesters of in-person data visualization courses with 30 students or less and was implemented in conjunction with Service-Learning (S-L). With the growth and popularity of the data visualization field—and the teaching environment created by the COVID-19 pandemic—more academic institutions are offering visualization courses online. Therefore, in this paper, we strengthen and validate the epistemological foundations of the DSLM framework by testing its (1) adaptability to online learning environments and conditions and (2) scalability to larger classes with up to 57 students. We present two online implementations of the DSLM framework, with and without Service-Learning (S-L), to test the adaptability and scalability of the framework. We further demonstrate that the framework can be applied effectively without the S-L component. We reflect on our experience with the online DSLM implementations and contribute a detailed retrospective analysis using thematic analysis and grounded theory methods to draw valuable recommendations and guidelines for future applications of the framework. This work verifies that DSLM can be used successfully in online classes to teach design study methodology. Finally, we contribute novel additions to the DSLM framework to further enhance it for teaching and learning design studies in the classroom. The preprint and supplementary materials for this paper can be found at https://osf.io/6bjx5/. 相似文献
395.
Aaron C. Spahr Marina E. Michaud Lauren E. Amoo Christian A. Sanchez Cody E. Hogue Laura M. Thierer Michael R. Gau William M. Wuest Prof. Kevin P. C. Minbiole 《ChemMedChem》2022,17(14):e202200224
Biscationic quaternary phosphonium compounds (bisQPCs) represent a promising class of antimicrobials, displaying potent activity against both Gram-negative and Gram-positive bacteria. In this study, we explored the effects of structural rigidity on the antimicrobial activity of QPC structures bearing a two-carbon linker between phosphonium groups, testing against a panel of six bacteria, including multiple strains harboring known disinfectant resistance mechanisms. Using simple alkylation reactions, 21 novel compounds were prepared, although alkene isomerization as well as an alkyne reduction were observed during the respective syntheses. The resulting bisQPC compounds showed strong biological activity, but were hampered by diminished solubility of their iodide salts. One compound (P2P-10,10 I) showed single-digit micromolar activity against the entire panel of bacteria. Overall, intriguing biological activity was observed, with less rigid structures displaying better efficacy against Gram-negative strains and more rigid structures demonstrating slightly increased efficacy against S. aureus strains. 相似文献
396.