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81.
Porous glass packing materials have been used for gel permeation chromatography using an aqueous phosphate buffer. Elution volumes were determined for polystyrene sulfonates, dextrans, and small neutral and charged molecules at three different ionic strengths, viz., 0.01M, 0.1M, and 1.0M phosphate, pH 7.0. The pore diameters of the glasses studied were 75, 240, 700, and 2000 Å. Elution volumes of nonionic species were unaffected by changing the solvent ionic strength. Elution volumes of charged species were markedly affected by the ionic strength of the solvent. This was attributed to a combination of decreased polymer dimensions and decreased ionic exclusion with increasing buffer concentration. The use of low ionic strength solvents may be exploited to tailor the separating range for polyelectrolytes with porous glass packings. This is particularly useful in the low molecular weight range where the lowest pore size available is 75 Å. 相似文献
82.
Estimation of Defects Based on Defect Decay Model: ED^{3}M 总被引:1,自引:0,他引:1
Haider S.W. Cangussu J.W. Cooper K.M.L. Dantu R. 《IEEE transactions on pattern analysis and machine intelligence》2008,34(3):336-356
An accurate prediction of the number of defects in a software product during system testing contributes not only to the management of the system testing process but also to the estimation of the product's required maintenance. Here, a new approach called ED3M is presented that computes an estimate of the total number of defects in an ongoing testing process. ED3M is based on estimation theory. Unlike many existing approaches the technique presented here does not depend on historical data from previous projects or any assumptions about the requirements and/or testers' productivity. It is a completely automated approach that relies only on the data collected during an ongoing testing process. This is a key advantage of the ED3M approach, as it makes it widely applicable in different testing environments. Here, the ED3M approach has been evaluated using five data sets from large industrial projects and two data sets from the literature. In addition, a performance analysis has been conducted using simulated data sets to explore its behavior using different models for the input data. The results are very promising; they indicate the ED3M approach provides accurate estimates with as fast or better convergence time in comparison to well-known alternative techniques, while only using defect data as the input. 相似文献
83.
Liu S Kang K Tarel JP Cooper DB 《IEEE transactions on pattern analysis and machine intelligence》2008,30(1):131-146
In this paper, the duality in differential form is developed between a 3D primal surface and its dual manifold formed by the surface's tangent planes, i.e., each tangent plane of the primal surface is represented as a four-dimensional vector which constitutes a point on the dual manifold. The iterated dual theorem shows that each tangent plane of the dual manifold corresponds to a point on the original 3D surface, i.e., the dual of the dual goes back to the primal. This theorem can be directly used to reconstruct 3D surface from image edges by estimating the dual manifold from these edges. In this paper we further develop the work in our original conference papers resulting in the robust differential dual operator. We argue that the operator makes good use of the information available in the image data, by using both points of intensity discontinuity and their edge directions; we provide a simple physical interpretation of what the abstract algorithm is actually estimating and why it makes sense in terms of estimation accuracy; our algorithm operates on all edges in the images, including silhouette edges, self occlusion edges, and texture edges, without distinguishing their types (thus resulting in improved accuracy and handling locally concave surface estimation if texture edges are present); the algorithm automatically handles various degeneracies; and the algorithm incorporates new methodologies for implementing the required operations such as appropriately relating edges in pairs of images, evaluating and using the algorithm's sensitivity to noise to determine the accuracy of an estimated 3D point. Experiments with both synthetic and real images demonstrate that the operator is accurate, robust to degeneracies and noise, and general for reconstructing free-form objects from occluding edges and texture edges detected in calibrated images or video sequences. 相似文献
84.
There is an increasing use of computer media for negotiations. However, the use of computer-mediated channels increases the hostile expressions of emotion, termed flaming. Although researchers agree that flaming has important effects on negotiation, predictions concerning these effects are inconsistent, suggesting a need for further investigation. We address this need by extending current flaming and negotiation research in two ways. First, we identify two different types of flaming: that which is motivated by perceptions concerning the negotiating opponent (e.g., he/she is unfair) and that which is motivated by perceptions concerning the negotiating context (e.g., the communication channel is too slow). Second, we differentiate between the effects of flaming on the concession behaviors of the flame sender and the flame recipient, and the effects of these behaviors on negotiated agreement. Via a laboratory study, we demonstrate that flames directed at the negotiation opponent slightly decrease the likelihood of reaching an agreement, and when an agreement is reached, it result in outcomes significantly favoring the flame recipient rather than the flame sender. In contrast, flames directed at the negotiation context significantly increase the likelihood of agreement, although outcomes still favor the flame recipient over the flame sender. These results suggest that flame senders are generally worse off than flame recipients, which provides an important basis for the strategic use of flaming in negotiations. 相似文献
85.
M. V. Klein Ran Liu D. Salamon S. L. Cooper W. C. Lee D. M. Ginsberg S. -W. Cheong 《Journal of Superconductivity》1994,7(2):429-433
Optical absorption at the insulating gap in the parent phase of cuprate superconductors shows a broad exciton-like peak near 1.7 eV, followed by a gradual decrease in absorption persisting 1 eV above the gap. By using ultraviolet laser lines to excite Raman spectra, we have found a Raman peak 0.2 eV below the first absorption peak in insulating cuprates. The Raman peak is much narrower than the absorption peak and hasA
2g
symmetry. We assign it to an exciton consisting of a hole transition from Cu
to a linear combination of Cud
xy
and nearest neighbor Op
orbitals. We have also studied the resonance Raman profile for two-magnon Raman scattering in the same samples. We find a sharp resonance feature at about 2.7 eV, and little Raman intensity for photon energies at the 1.7 eV absorption peak. The state created at the peak must therefore be an inappropriate intermediate state for the double spin-flip Raman process. 相似文献
86.
Marquardt CL Cooper DG Budni PA Knights MG Schepler KL Dedomenico R Catella GC 《Applied optics》1994,33(15):3192-3197
We performed an experimental investigation of thermal lensing in silver gallium selenide (AgGaSe(2)) optical parametric oscillator crystals pumped by a 2-μm laser at ambient temperature. We determined an empirical expression for the effective thermal focusing power in terms of the pump power, beam diameter, crystal length, and absorption coefficient. This relation may be used to estimate average power limitations in designing AgGaSe(2) optical parametric oscillators. We also demonstrated an 18% slope efficiency from a 2-μm pumped AgGaSe(2) optical parametric oscillator operated at 77 K, at which temperature thermal lensing is substantially reduced because of an increase in the thermal conductivity and a decrease in the thermal index gradient dn/dT. Cryogenic cooling may provide an additional option for scaling up the average power capability of a 2-μm pumped AgGaSe(2) optical parametric oscillator. 相似文献
87.
MB Hossain D van der Helm FJ Schmitz EO Pordesimo RA Magarian KL Meyer LB Overacre BW Day 《Canadian Metallurgical Quarterly》1994,37(11):1670-1683
Molecular structures and conformational characteristics of a series of 1,1-dichloro-2,2,3-triarylcyclopropanes (DTACs), which were reported previously to be distinctly antiestrogenic and inhibitors of the estrogen-receptor-positive MCF-7 human breast cancer cells in culture, are reported. In addition, structural and conformational features of the DTACs were compared to the first-known nonsteroidal antiestrogen, MER25, and the clinically useful antiestrogen Tamoxifen. The molecular structures of four DTAC compounds were determined by X-ray diffraction. Crystallographic structures show that the DTAC molecules have nearly the same relative conformation for the three aryl rings which is designated as a "nonpropeller" conformation in contrast to the observed "propeller" conformation for the three rings in all known triarylethylenes. Systematic conformational searches were performed to find the conformational preferences of DTACs, MER25, and Tamoxifen using idealized model compounds built from their respective crystal structure. Energy-minimization and conformational-search studies demonstrated that all DTAC molecules have a common, single global minimum energy conformer for their central core containing the dichlorotriarylcyclopropyl system, which is similar to that found in their crystal structures. Conformational search of MER25 showed that the molecule can assume a number of low-energy conformers of which two, one anti (A1) and one gauche (G1A), have about the same energy. The anti conformation is similar to the one observed in its crystal structure and resembles the estrogenic E-isomer of Tamoxifen, while the lowest energy gauche conformer of MER25 resembles more closely the antiestrogenic Z-isomer of Tamoxifen. NMR spectroscopic analysis of MER25 showed that the molecule exists predominantly in the anti conformation in solution. A comparative review of the structural features and bioactivities of Tamoxifen, DTACs, and MER25 provides a possible explanation for their low estrogen receptor binding affinity which is common to these compounds together with their antiestrogenic activity. 相似文献
88.
89.
JE is a member of the family of "immediate early" genes induced by growth factors and cytokines. JE encodes a low molecular weight secretory glycoprotein analogous to the human monocyte chemoattractant protein, MCP-1. JE and MCP-1 proteins are thought to play an important role in inflammation and in the recruitment of monocyte/macrophages to the vessel wall during the development of atherosclerosis. We have previously reported that the induction of JE in rat aortic smooth muscle cells (SMC) was specific to platelet-derived growth factor (PDGF) and was not seen with other growth agonists. Using a luciferase reporter system and transient transfection assays of rat aortic SMC, we now report the identification of a region in the proximal rat JE promoter that is responsive to PDGF but not to other growth factors (angiotensin II and alpha-thrombin) or cytokines (interleukin 1-beta and tumor necrosis factor-alpha). The full response to PDGF (approximately 6-fold) requires the cooperative activity of two potentially novel cis-acting elements, at positions -146 to -128 and -84 to -59. While each element produces a different pattern in electrophoretic mobility shift assays, they appear to bind the same PDGF-responsive species. Further analysis of these regions should provide important insights into PDGF-specific responses in vascular SMC. 相似文献
90.
JB Hale JA Hoeppner MB DeWitt DL Coury DG Ritacco B Trommer 《Canadian Metallurgical Quarterly》1998,31(6):595-607
We introduce a novel application for linkage analysis: using bone marrow donor-recipient sib pairs to search for genes influential in graft-versus-host disease (GVHD), a major cause of morbidity and mortality following allogeneic bone marrow transplantation. In particular, we show that transplant sib pairs in which the recipient developed severe GVHD can be used to map genes in the same way as traditional discordant (affected/unaffected) sib pairs (DSPs). For a plausible GVHD model, we demonstrate that the transplant/discordant sib pair analog of the "possible triangle test" [Holmans (1993) Am J Hum Genet 52:362-374] has similar power to that of the simpler "restricted test" proposed by Risch [(1990b) Am J Hum Genet 46:229-241; (1992) Am J Hum Genet 51:673-675]. Moreover, we show that the restricted test has superior power in much of the DSP possible triangle and significantly inferior power in only a small region. Thus, we conclude that the restricted test is preferable for localizing genes with transplant/discordant sib pairs. Finally, we examine the effects of heterogeneity on the power to detect GVHD loci and demonstrate the gain in efficiency by dividing the sample into genetically more homogeneous subgroups. 相似文献