Comparisons of the low-resolution structures of ornithine decarboxylase by electron microscopy and X-ray crystallography: The utility of methylamine tungstate stain and butvar support film in the study of macromolecules by transmission electron microscopy

The structure of ornithine decarboxylase (M_r ≈? 1.04 × 10⁶) from Lactobacillus 30a was investigated by electron microscopy and x-ray crystallography. Electron micrographs showed the structure to be well preserved in methylamine tungstate stain. The molecules interacted little with the Butvar support film, yielding three unique projections: a hexagonal ring (front view) and two rod-shaped projections (edge views). Stereo pairs revealed a novel feature of the Butvar film in that some molecules were suspended in the stain in random orientations. Consequently, the relatedness of the hexagonal ring and the rod-shaped particles could be demonstrated since some particle shapes interconverted when the stage was tilted ± 45°. The two edge views were related by a 30° rotation about the sixfold axis. Image averaging of the three primary views suggested a dodecamer (point group symmetry 622) composed of two hexameric rings, apparently in an eclipsed configuration. To investigate the structural organization of the complex, the dissociation of the enzyme was studied by electron microscopy. The dissociation process involved the initial breakage of the ring followed by separation of dimers from the ring (one subunit from each of the two hexamers). Thus, the dodecamer forms as a hexamer of dimers rather than a dimer of hexamers. These structural studies were confirmed and extended by x-ray crystallographic analysis. A 4.0-Å resolution electron density map revealed two hexameric rings, consisting of six closely associated dimers, tilted approximately 10° with respect to the molecular twofold axis. Electron density projections of the three primary views of the molecule derived from the x-ray data corresponded closely to those obtained from image averaging of the electron microscopy data, thereby establishing in a novel way the reliability of the electron microscopy studies. Methylamine tungstate stain and Butvar support film therefore offer unique advantages for investigating protein structures by electron microscopy.     

Chromogenic Photonic Crystal Sensors Enabled by Multistimuli‐Responsive Shape Memory Polymers

Here novel chromogenic photonic crystal sensors based on smart shape memory polymers (SMPs) comprising polyester/polyether‐based urethane acrylates blended with tripropylene glycol diacrylate are reported, which exhibit nontraditional all‐room‐temperature shape memory (SM) effects. Stepwise recovery of the collapsed macropores with 350 nm diameter created by a “cold” programming process leads to easily perceived color changes that can be correlated with the concentrations of swelling analytes in complex, multicomponent nonswelling mixtures. High sensitivity (as low as 10 ppm) and unprecedented measurement range (from 10 ppm to 30 vol%) for analyzing ethanol in octane and gasoline have been demonstrated by leveraging colorimetric sensing in both liquid and gas phases. Proof‐of‐concept tests for specifically detecting ethanol in consumer medical and healthcare products have also been demonstrated. These sensors are inexpensive, reusable, durable, and readily deployable with mobile platforms for quantitative analysis. Additionally, theoretical modeling of solvent diffusion in macroporous SMPs provides fundamental insights into the mechanisms of nanoscopic SM recovery, which is a topic that has received little examination. These novel sensors are of great technological importance in a wide spectrum of applications ranging from environmental monitoring and workplace hazard identification to threat detection and process/product control in chemical, petroleum, and pharmaceutical industries.     

Advances in Spin Catalysts for Oxygen Evolution and Reduction Reactions

Searching for high effective catalysts has been an endless effort to improve the efficiency of green energy harvesting and degradation of pollutants. In the past decades, tremendous strategies are explored to achieve high effective catalysts, and various theoretical understandings are proposed for the improved activity. As the catalytic reaction occurs at the surface or edge, the unsaturated ions may lead to the fluctuation of spin. Meanwhile, transition metals in catalysts have diverse spin states and may yield the spin effects. Therefore, the role of spin or magnetic moment should be carefully examined. In this review, the recent development of spin catalysts is discussed to give an insightful view on the origins for the improved catalytic activity. First, a brief introduction on the applications and advances in spin-related catalytic phenomena, is given, and then the fundamental principles of spin catalysts and magnetic fields-radical reactions are introduced in the second part. The spin-related catalytic performance reported in oxygen evolution/reduction reaction (OER/ORR) is systematically discussed in the third part, and general rules are summarized accordingly. Finally, the challenges and perspectives are given. This review may provide an insightful understanding of the microscopic mechanisms of catalytic phenomena and guide the design of spin-related catalysts.     

Bus passenger flow statistics algorithm based on deep learning

Multimedia Tools and Applications - Bus passenger flow statistics can be used to improve passenger travelling experience and reduce trip delay, this is very important for intelligent...     

Optimization of Digital Growth of Thick N-Polar InGaN by MOCVD

Smooth 200 nm thick N-polar InGaN films were grown by metal–organic chemical vapor deposition (MOCVD) on sapphire using a digital approach consisting of a constant In, Ga, and N precursor flow with pulsed injection of H2 into the N2 carrier gas. Using this growth scheme, the H2 injection time was altered and the effect on the morphology and indium incorporation in the films observed. The effect of periodic insertion of additional GaN inter-layers on the surface morphology of the InGaN layers was also studied.     

Site-directed and deletion mutational analysis of the receptor binding domain of the interleukin-6 receptor targeted fusion toxin DAB389-IL-6

We have used site-directed and in-frame deletion mutationalanalysis in order to explore the structural features of theIL–6 portion of the diphtheria toxin-related interleukin–6(IL–6) fusion toxin DAB₃₈₉-IL–6 that are essentialfor receptorbinding and subsequent inhibition of protein synthesisin target cells. Deletion of the first 14 amino acids of theIL–6 component of the fusion toxin did not alter eitherreceptor binding affinity or cytotoxk potency. In contrast,both receptor binding and cytotoxic activity were abolishedwhen the C–terminal 30 amino acids of the fusion toxinwere deleted. In addition, we explored the relative role ofthe disulfide bridges within the IL–6 portion of DAB₃₈₉-IL–6in the stabilization of structure required for receptor-binding.The analysis of mutants in which the substitution of eitherCys⁴⁴⁰, Cys⁴⁴⁶, Cys⁴⁶⁹ or Cys⁴⁷⁹ to Ser respectively, demonstratesthat only the disulfide bridge between Cys⁴⁶⁹ and Cys⁴⁷⁹ isrequired to maintain a functional receptor binding domain. Inaddition, the internal in-frame deletion of residues 435–451,which includes Cys⁴⁴⁰ and Cys⁴⁴⁶, was found to reduce, but notabolish receptor binding affinity. These results further demonstratethat the disulfide bridge between Cys⁴⁴⁰ and Cys⁴⁴⁶ is not essentialfor receptor-binding. However, the reduced cytotoxic potencyof DAB₃₈₉-IL6(435–451) suggests that the conformationand/or receptor binding sites associated with this region ofthe fusion toxin is/are important for maintaining the wild typereceptor binding affinity and cytotoxic potency.     

Inefficiencies in electricity pricing in England and Wales

The combination of competition and regulation established in the electricity industry of England and Wales stimulates production efficiency and thus helps the industry to achieve low costs. Nevertheless this system does have some disadvantages as regards the incentives it presents to generators and to suppliers. These deficiencies arise because Use of System Charges both for Distribution and for Transmission are determined in a way that inadequately reflects the structure and level of marginal costs. Furthermore, the way that profiling for consumers taking less than 100 kW will work is likely to inhibit tariff innovation.     

Optical quorum cycles for efficient communication

Many optical networks face heterogeneous communication requests requiring topologies to be efficient and fault tolerant. For efficiency and distributed control, it is common in distributed systems and algorithms to group nodes into intersecting sets referred to as quorum sets. We show efficiency and distributed control can also be accomplished in optical network routing by applying the same established quorum set theory. Cycle-based optical network routing, whether using SONET rings or p-cycles, provides the sufficient reliability in the network. Light-trails forming a cycle allow broadcasts within a cycle to be used for efficient multicasts. Cyclic quorum sets also have all pairs of nodes occurring in one or more quorums, so efficient, arbitrary unicast communication can occur between any two nodes. Efficient broadcasts to all network nodes are possible by a node broadcasting to all quorum cycles to which it belongs (\(O(\sqrt{N})\)). In this paper, we propose applying the distributed efficiency of the quorum sets to routing optical cycles based on light-trails. With this new method of topology construction, unicast and multicast communication requests do not need to be known or even modeled a priori. Additionally, in the presence of network link faults, greater than 99 % average coverage enables the continued operation of nearly all arbitrary unicast and multicast requests in the network. Finally, to further improve the fault coverage, an augmentation to the ECBRA cycle finding algorithm is proposed.     

Dual interband cascade laser based trace-gas sensor for environmental monitoring

The development of an interband cascade laser (ICL) based spectroscopic trace-gas sensor for the simultaneous detection of two atmospheric trace gases is reported. The sensor performance was evaluated using two ICLs capable of targeting formaldehyde (H2CO) and ethane (C2H6). Minimum detection limits of 3.5 ppbV for H2CO and 150 pptV for C2H6 was demonstrated with a 1 s integration time. The sensor was deployed for field measurements of H2CO, and laboratory quantification of both formaldehyde and ethane are reported. A cross comparison of the atmospheric concentration data for H2CO with data collected by a collocated commercial H2CO sensor employing Hantzsch reaction based fluorometric detection was performed.These results show excellent agreement between these two different approaches for trace-gas quantification. In addition, laboratory experiments for dual gas quantification show accurate, fast response with no crosstalk between the two gas channels.