Customization of a Commercially Available Prep Scale SFC System to Provide Enhanced Capabilities

Preparative Scale Supercritical Fluid Chromatography is emerging as a powerful alternative to HPLC for the purification and separation of complex chemical reaction mixtures. Advantages include greatly reduced solvent usage (and thus lower cost and environmental impact), higher throughput, and in some cases higher resolution. While there are commercially available prep SFC instruments, none currently offer all the features desired by many medicinal chemists engaged in the drug discovery process. These include: the ability to collect an unlimited number of fractions per sample with high recovery and negligible carryover, fully automated capacity to collect several hundred fractions, and the ability to collect fractions into the same disposable test tubes and racks which are already employed in HPLC. This article describes the customization of a preparatory scale SFC system purchased from Berger Instruments, Inc., Newark, DE. (a subsidiary Mettler-Toledo International, Inc., of Greifensee, Switzerland) in order to provide these capabilities.     

Acceptability of voluntary HIV testing by pregnant women in developing countries: an international survey. Ghent International Working Group on Mother-to-Child Transmission of HIV

OBJECTIVE: To evaluate acceptability of voluntary HIV counselling and testing (VCT) by pregnant women in the context of clinical trials assessing interventions to reduce mother-to-child transmission (MCT) of HIV in developing countries. METHODS: During September-October 1997, 13 studies located in West (Abidjan, Bobo Dioulasso), East (Nairobi, Mombasa, Dar Es Salaam, Blantyre, Lusaka, Harare) and South Africa (Soweto, Durban), and Thailand (Bangkok) were included in a cross-sectional mailing survey about the acceptability of VCT in antenatal clinics. Acceptance rate, return rate, overall acceptability of VCT (acceptance of both pre- and post-VCT sessions) were obtained using a standardized questionnaire. RESULTS: The median overall acceptability of VCT was 69% (range, 33-95%). Overall acceptability of VCT most frequently depended on return rates because acceptance rates were generally high. Where several studies were conducted in parallel in the same city or the same country, overall acceptability rates of HIV testing were generally comparable even if the intervention programmes differed. Overall acceptability rates of VCT were high in antenatal clinics where a particular effort in implementing VCT programmes had been made. CONCLUSIONS: This international survey shows that despite many obstacles, VCT is feasible and acceptable for pregnant women aiming to reduce their risk of transmitting HIV to their children.     

Update on methodology topics

The following column on methodology topic was prepared by AOCS Technical Director Dave Berner. Questions on these or other topics concerning analytical methodology of fats and oils or related products may be directed to Dave Berner, AOCS, PO Box 3489, Champaign, IL 61821-0489, USA     

International intercomparison of horn gain at X-band

An international intercomparison of horn gain and polarization measurements at X-band has previously been completed. There were seven participating laboratories with the National Institute of Standards and Technology serving as the pilot laboratory. Two X-band pyramidal standard gain horns with a nominal gain of 22 dB served as the traveling standards. Quantities measured included on-axis fixed frequency gain at 8, 10, and 12 GHz, swept frequency gain between 8-12 GHz and polarization characteristics at the three fixed frequencies. All laboratories performed the fixed frequency-gain measurements. The swept-frequency and polarization measurements were optional, with four laboratories performing swept-frequency measurements and three laboratories measuring polarization. The results of the gain measurements generally agreed within the reported uncertainties which were of the order of 0.1 dB or less     

Efficient testing of segmented aspherical mirrors by use of a reference plate and computer-generated holograms. II. Case study, error analysis, and experimental validation

Segmented mirrors present unique challenges to fabrication and testing that are absent for monolithic optics. Since traditional asphere tests do not address segmented optics adequately, we validate a previously developed method to test large quantities of segments accurately, quickly, and economically. In this test, the aspheric shape of each segment is controlled to high accuracy by use of computer-generated holograms, and the radius of curvature is tightly controlled by use of the reference plate. In an adjoining paper [Appl Opt 43, 5303 (2004)] we developed the theory for this test, and now we present a complete system design and optimization for measuring the 1.4-m segments from a 30-m F/1 primary. A complete tolerance analysis predicts a test accuracy of 4.8-nm rms surface and excellent accuracy for controlling the geometry of the segment. In addition, a laboratory demonstration using 30-cm optics is presented that demonstrated 3.9-nm rms surface accuracy.     

A non-lensed fiber-optic resonance-enhanced multiphoton ionization probe

We have developed a miniature fiber-optic probe with no focusing optics for in situ analysis of volatile organic compounds (VOCs). The probe uses an optical fiber to transmit a laser pulse to a vapor sample causing it to ionize adjacent to the fiber tip through a resonance-enhanced multiphoton ionization (REMPI) process. The distal end of the optical fiber is contained co-axially within 2-mm-inner-diameter stainless steel tubing that serves as an electrode. The electrode is biased at a high positive potential to collect electrons. The current generated is shown to be proportional over about two orders of magnitude to the concentration of the species ionized. Visible wavelength REMPI spectroscopy is used to determine probe sensitivities of 20 ppb (benzene) and 43 ppb (toluene). Designing the probe without focusing optics specifies an achromatic ionization region constant in size and position as the laser wavelength is scanned, which simplifies data collection and reduction. Focusing achromatic systems are discussed and the potential signal improvement is estimated.     

Early user involvement in the development of information technology-related products for older people

The UTOPIA project (Usable Technology for Older People—Inclusive and Appropriate) is focused on developing effective methods for the early involvement of older people in the development of information technology-related products for people aged 60 and over, and on providing industry with tools to assist in the development of information technology products for such older people. An essential part of this methodology is building a diverse user base, forming a long-lasting partnership with older people, and developing approaches for effective interaction with this target user group. Our experiences with eliciting information from groups of older people about technology is described, together with a report on seminars for Scottish industry designed to raise an awareness of these issues.     

A central cholinergic inhibitory system as a basis for tonic immobility (animal hypnosis) in chickens.

4 experiments provided support for the hypothesis that tonic immobility (TI) in chickens, which may be analogous to response suppression in the rat, is a result of fear and is mediated by central cholinergic systems. A total of 124 experimental and 54 control, White Leghorn cockerel chicks were the Ss. Exp I established that scopolamine, a central and peripheral acting anticholinergic, will reduce the duration of TI, whereas methylscopolamine, which acts only peripherally, will not. Exp II established a dose-response curve for scopolamine and TI. Exp III demonstrated that scopolamine increased activity and that this increase may be a factor in reducing the duration of TI. Exp IV showed that physostigmine, which blocks acetylcholinesterase and allows longer action of acetylcholine at the synapse, increased the duration of TI. (18 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

电炉JetBOxTM技术的发展

美国PTI公司发明的专利技术JetBOxTM超音速氧枪喷射系统被证明是一种能有效提高电弧炉产量同时又能减少电炉改造成本的技术.通过在各个炼钢电炉上使用这种独特而有效的化学能输入方式从而获得了良好的效果.由于每个电炉的JetBOxTM系统都是专门设计,能满足每个炼钢车间的不同要求,因此已经被成功地应用在用料篮加料的普通电炉、Consteel电炉、使用DRI/HBI的电炉以及热装铁水的电炉上,同样都获得了良好的运行效果.自2001年以来JetBOxTM系统已经在全世界超过60台不同的电炉上安全而有效地运行着,满足了不同钢厂的要求.