A new family of yeast genes implicated in ergosterol synthesis is related to the human oxysterol binding protein

We have identified three yeast genes, KES1, HES1 and OSH1, whose products show homology to the human oxysterol binding protein (OSBP). Mutations in these genes resulted in pleiotropic sterol-related phenotypes. These include tryptophan-transport defects and nystatin resistance, shown by double and triple mutants. In addition, mutant combinations showed small but apparently cumulative reductions in membrane ergosterol levels. The three yeast genes are also functionally related as overexpression of HES1 or KES1 alleviated the tryptophan-transport defect in kes1Δ or osh1Δ mutants, respectively. Our study implicates this new yeast gene family in ergosterol synthesis and provides comparative evidence of a role for human OSBP in cholesterol synthesis.     

Routine Highway Bridge Inspection Condition Documentation Accuracy and Reliability

Routine inspection is the most common form of highway bridge inspection to satisfy the requirements of the National Bridge Inspection Standards. The accuracy and reliability of documentation generated during these inspections are critical to the allocation of Department of Transportation construction, maintenance, and rehabilitation resources. Routine inspections are typically completed using only the visual inspection technique and rely heavily on subjective assessments made by bridge inspectors. In light of this, and given the fact that visual inspection may have other limitations that influence its reliability, the Federal Highway Administration initiated an investigation to examine the reliability of visual inspection as it is currently applied to bridges in the United States. This paper will summarize results from this study related to the accuracy and reliability of routine inspection documentation. A number of important conclusions were developed from the experimental study. Generally, it was found that all structural condition documentation is collected with significant variability. Specifically, 95% of primary element condition ratings for individual bridge components will vary within two rating points of the average and only 68% will vary within one point. Documentation generally collected to support condition ratings also has significant variability as exemplified by the number and types of field notes and photographs taken by inspectors. With respect to the use of element-level inspections, it was found that element usage was generally consistent with the Commonly Recognized Element Guide. However, there is significant variability in the condition state assignments of those elements and in some cases the condition states are not applied correctly to particular elements.     

Life stress and adjustment: Effects of life events experienced by young adolescents and their parents.

A longitudinal design was used to test the effects of life events experienced by young adolescents and their parents. The criteria were the adolescents' depression, anxiety, and self-esteem. The longitudinal analysis revealed a significant effect for the adolescents' controllable, but not uncontrollable, negative events. However, causal analyses revealed that this effect was the result of the significant relation between initial adjustment and the subsequent occurrence of controllable life stress (e.g., school suspension). The longitudinal analysis also revealed the stress-protective role of positive events, but only with respect to girls' self-esteem. There was no longitudinal support for the role of the parents' negative life events. These findings do not support the etiological importance of an accumulation of relatively discrete negative events experienced by early adolescents and their parents, but they do suggest the need (a) to conceptualize (controllable) life stress as a dependent variable in future research on developmental psychopathology; (b) to examine gender differences in early adolescent life stress; and (c) to develop more sophisticated measures of family life stress. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Multifactorial assessment of bulimia nervosa.

Investigated a multifactorial approach to the assessment of bulimia nervosa by means of hierarchical factor analysis. 245 bulimia nervosa patients and 68 patients with either anorexia nervosa or eating disorders not otherwise specified were administered a self-report battery that was organized into 21 dimensions relevant to eating disorder patients. When dimensions from this battery were subjected to hierarchical factor analysis, support for bulimia nervosa as a unique diagnostic category was obtained. However, the emergence of 3 secondary factors and 6 primary factors suggests that bulimia nervosa can also be described more complexly. The emergence of a multifactorial model of bulimia nervosa that incorporates several existing unidimensional models suggests the potential for both divergent and complicated clinical presentation in bulimia nervosa patients. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Observations on the training of therapists in time-limited dynamic psychotherapy.

Suggests that "manualized training" is most effective at increasing adherence to the technical procedures characterizing the treatment under scrutiny. There is minimal evidence that other skills associated with therapeutic competence are acquired or enhanced to the extent found with technical adherence. Drawing on findings from a study of manual-guided training of therapists in the techniques of brief therapy (H. H. Strupp and J. L. Binder, 1984), the author offers recommendations for improving therapy training. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Children's Physiological Indices of Empathy and Their Socioemotional Adjustment: Does Caregivers' Expressivity Matter?

Relations of heart rate and skin conductance reactions to mildly evocative empathy-inducing slides with socioemotional functioning were examined for 154 children (mean age = 9 years, 5 months). In addition, maternal expressivity was tested as a moderator of these relations. Parents and teachers rated children's socioemotional functioning, and a behavioral measure of children's regulation was obtained. Boys who exhibited higher skin conductance and higher heart rate to slides depicting negative emotions were better regulated, less emotionally intense, and better adjusted than their peers. Furthermore, boys' regulation and adjustment were positively related to such physiological responding to negative slides if maternal negative expressivity was relatively low or moderate, but not high. Fewer findings were obtained for girls or for positive slides. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Agricultural land-use change in Brazilian Amazônia between 1980 and 1995: Evidence from integrated satellite and census data

As cropland and pasture have replaced forest and cerrado in Brazilian Amazônia, concern has mounted over the effects of changing the biogeochemical and hydrological properties of one of the world's great storehouses of biomass and biodiversity. Although much recent effort has focused on the location, effects, and causes of deforestation and cerrado conversion, much less is known about the basin-wide spatial distribution and density of the land use following conversion for crops or pasture.In this paper, we use census and satellite records to develop maps of the distribution and abundance of major agricultural land uses across 4.5×10⁸ ha of Brazilian Amazônia in 1980 and 1995. Results indicate an overall expansion of 7.0×10⁶ ha in total agricultural area in Brazilian Amazônia between 1980 and 1995. The net change during this period is estimated for three different land-use types: croplands (an increase of 0.8×10⁶ ha), natural pastures (a decrease of 8.4×10⁶ ha), and planted pastures (an increase of 14.7×10⁶ ha). These estimates, the first spatially explicit quantifications of agricultural land-use activities in 1980 and 1995 across Brazilian Amazônia, are shown to be consistent with the results of applying a land use change and secondary regrowth model to published deforestation rates for the period.The resulting time slices, presented for each land-use category at 5-min (∼9 km) spatial resolution, allow for the quantification of land-use changes in this region for biogeochemical, demographic and economic models. Several foci of agricultural change existed within Brazilian Amazônia during this period: in the state of Pará, cropland was lost and planted pasture increased markedly; in Mato Grosso, both cropland and planted pasture increased; in Rondônia, planted pasture replacing forest was the primary route to agricultural expansion.     

Insiders Behaving Badly

This column goes beyond previous insider analyses to identify a framework for a taxonomy of insider threats including both malicious and inadvertent actions by insiders that put organizations or their resources at some risk. The framework includes factors reflecting the organization, the individual, the information technology system, and the environment.