相对刚度对圆形隧道结构地震反应影响规律的研究

隧道结构与周围地层的相对刚度是两者之间相互作用的根本,本文针对圆形隧道,采用动力有限元方法分别讨论了土质条件(剪切波速)、衬砌厚度、衬砌直径等参数对结构地震反应的影响规律。然后引入柔度比的概念来综合考虑上述因素的影响。计算结果显示:柔度比越大,隧道结构的地震内力反应越小,并且对计算结果进行拟合得到衬砌内力与柔度比的函数关系,可以作为隧道结构抗震分析、设计的参考。     

Preclinical Assessment of the Combination of PSMA-Targeting Radionuclide Therapy with PARP Inhibitors for Prostate Cancer Treatment

Prostate specific membrane antigen targeted radionuclide therapy (PSMA-TRT) is a promising novel treatment for prostate cancer (PCa) patients. However, PSMA-TRT cannot be used for curative intent yet, thus additional research on how to improve the therapeutic efficacy is warranted. A potential way of achieving this, is combining TRT with poly ADP-ribosylation inhibitors (PARPi), which has shown promising results for TRT of neuroendocrine tumor cells. Currently, several clinical trials have been initiated for this combination for PCa, however so far, no evidence of synergism is available for PCa. Therefore, we evaluated the combination of PSMA-TRT with three classes of PARPi in preclinical PCa models. In vitro viability and survival assays were performed using PSMA-expressing PCa cell lines PC3-PIP and LNCaP to assess the effect of increasing concentrations of PARPi veliparib, olaparib or talazoparib in combination with PSMA-TRT compared to single PARPi treatment. Next, DNA damage analyses were performed by quantifying the number of DNA breaks by immunofluorescent stainings. Lastly, the potential of the combination treatments was studied in vivo in mice bearing PC3-PIP xenografts. Our results show that combining PSMA-TRT with PARPi did not synergistically affect the in vitro clonogenic survival or cell viability. DNA-damage analysis revealed only a significant increase in DNA breaks when combining PSMA-TRT with veliparib and not in the other combination treatments. Moreover, PSMA-TRT with PARPi treatment did not improve tumor control compared to PSMA-TRT monotherapy. Overall, the data presented do not support the assumption that combining PSMA-TRT with PARPi leads to a synergistic antitumor effect in PCa. These results underline that extensive preclinical research using various PCa models is imperative to validate the applicability of the combination strategy for PCa, as it is for other cancer types.     

发酵大豆食品中染料木素含量的ELISA测定

染料木素是大豆发酵食品中的主要异黄酮成分。制备了牛血清白蛋白-染料木素免疫抗原和卵白蛋白-染料木素包被抗原,并对它们进行紫外和SDS-聚丙稀酰胺凝胶电泳鉴定。抗染料木素抗体的效价为1:1600。建立竞争ELISA测定发酵大豆制品中染料木素含量方法,染料木素测定浓度范围为6.25～150μg/L;回收率为87%～102%;亲和常数为1.62×108;与大豆甙元、芒柄花素的交叉反应率分别为9.8%、4.57%;与槲皮素、儿茶素、单宁和核黄素等结构类似物无交叉反应。同时将ELISA法与薄层层析法进行比较。     

超微皮革粉体表面改性的研究(续)

以超微粉碎皮革固体废弃物得到的超微皮革粉体为主要原料,先后用马来酸酐(MAH)、丙烯酸(AA)对其进行表面改性,得到了性能稳定的超微皮革粉体悬浮液。通过4因素3水平正交试验,分别以接枝率为指标探讨了MAH、AA改性超微皮革粉体的条件,得出最佳改性方案:在超微皮革粉体的N,N-二甲基甲酰胺(DMF)溶液中,加入50%(以超微皮革粉体质量计)的MAH,80℃恒温反应3h,得到改性产品Ⅰ,经过滤、水洗后以适量蒸馏水分散,以2%过硫酸铵(APS)溶液(以单体质量计)为引发剂,加入150%(以改性后超微皮革粉体质量计)的AA,温度为75℃,反应5h,得到最终改性产品。通过产品的红外光谱(FTIR)表征,表明接枝共聚反应的发生。     

瞬变电磁法在断层防治水中的应用

该文讨论了瞬变电磁法的原理,介绍了瞬变电磁法在阳城煤矿开拓大巷过季庄断层的应用设计施工,给出了季庄断层瞬变电磁法探测结果及结论,并根据结论采取了过断层的有效措施,通过巷道施工过程中的超前钻探及巷道实际揭露的断层的情况,验证了瞬变电磁法在断层防治水应用中可靠有效性。     

米糠油生理功能及制取工艺的研究

概述了米糠油的营养价值及生理功能,详细介绍了米糠制油技术及米糠油精炼技术,并分析了各种米糠油精炼技术的优缺点.     

Comparison and Characterization of Compounds with Antioxidant Activity in Lycium barbarum Using High‐Performance Thin Layer Chromatography Coupled with DPPH Bioautography and Tandem Mass Spectrometry

Methanol extracts from 50 batches of Lycium barbarum (L. barbarum, wolfberry) in China were compared and characterized using high‐performance thin‐layer chromatography coupled with 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) bioautography (HPTLC‐DPPH) and electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry (ESI‐Q‐TOF‐MS/MS), respectively. Results showed that similar components occupying the major antioxidant activity existed in L. barbarum collected from different origins. However, the average antioxidant capacities of methanol extracts of L. barbarum collected in Ningxia were significantly higher than those of Qinghai, Xinjiang, Inner Mongolia, and Gansu, which may contribute to rational use of L. barbarum in China. Furthermore, the chemical structure of compound with the highest antioxidant capacity was tentatively identified as 2‐O‐β‐d ‐glucopyranosyl‐l ‐ascorbic acid using ESI‐Q‐TOF‐MS/MS analysis, which possessed high potentials to be used as an antioxidant biomarker for the quality control of L. barbarum. Results are helpful for the bioactivity‐based quality control of L. barbarum, and beneficial for the improvement of their performance in functional/health foods area, suggesting that HPTLC‐DPPH bioautography with ESI‐Q‐TOF‐MS/MS could be used as a routine approach for quality control of antioxidant components in L. barbarum.     

苏子油多不饱和脂肪酸尿素富集研究

采用尿素富集法，使苏子油中多不饱和脂肪酸的含量由原来的７６．９％提高到９９．９％，亚麻酸含量由６４．７％提高到９０．９％。从而使脂肪酸的质量有了进一步的提高。     

城市森林公园消防规划设计

针对城市森林公园具有森林、建筑火灾的双重特点,按照消防设计的一般惯例和可借鉴的规范,并结合城市森林公园的实际情况,阐述了城市森林公园消肪规划设计应具备的项目和具体内容。     

制革污泥及施含铬污泥土壤中铬含量测定方法的研究

对浓H