Dynamic subset selection based on a fitness case topology

A large training set of fitness cases can critically slow down genetic programming, if no appropriate subset selection method is applied. Such a method allows an individual to be evaluated on a smaller subset of fitness cases. In this paper we suggest a new subset selection method that takes the problem structure into account, while being problem independent at the same time. In order to achieve this, information about the problem structure is acquired during evolutionary search by creating a topology (relationship) on the set of fitness cases. The topology is induced by individuals of the evolving population. This is done by increasing the strength of the relation between two fitness cases, if an individual of the population is able to solve both of them. Our new topology-based subset selection method chooses a subset, such that fitness cases in this subset are as distantly related as is possible with respect to the induced topology. We compare topology-based selection of fitness cases with dynamic subset selection and stochastic subset sampling on four different problems. On average, runs with topology-based selection show faster progress than the others.     

Software architecture awareness in long-term software product evolution

Software architecture has been established in software engineering for almost 40 years. When developing and evolving software products, architecture is expected to be even more relevant compared to contract development. However, the research results seem not to have influenced the development practice around software products very much. The architecture often only exists implicitly in discussions that accompany the development. Nonetheless many of the software products have been used for over 10, or even 20 years. How do development teams manage to accommodate changing needs and at the same time maintain the quality of the product? In order to answer this question, grounded theory study based on 15 semi-structured interviews was conducted in order to find out about the wide spectrum of architecture practices in software product developing organisations. Our results indicate that a chief architect or central developer acts as a ‘walking architecture’ devising changes and discussing local designs while at the same time updating his own knowledge about problematic aspects that need to be addressed. Architecture documentation and representations might not be used, especially if they replace the feedback from on-going developments into the ‘architecturing’ practices. Referring to results from Computer Supported Cooperative Work, we discuss how explicating the existing architecture needs to be complemented by social protocols to support the communication and knowledge sharing processes of the ‘walking architecture’.     

Hexadecylphosphocholine and 2-modified 1,3-diacylglycerols as effectors of phospholipase D

The effects of mesulergine (100 and 200 microg/kg s.c.), SB 206553 (1 and 2.5 mg/kg i.p.), RP 62203 (2.5 and 4 mg/kg i.p.) and ritanserin (630 microg/kg i.p.) were studied on the extracellular concentration of dopamine (DA) and dihydroxyphenylacetic acid (DOPAC) in the nucleus accumbens of chloral hydrate-anesthetized rats, using intracerebral microdialysis. Mesulergine, a non selective serotonin2C/2B/2A (5-HT2C/2B/2A) receptor antagonist, significantly increased DA release, which reached a peak level (+ 20%) 60 min after drug injection and slowly returned back to baseline values. Mesulergine also caused a dose-dependent increase in DOPAC outflow. Pretreatment with mesulergine (200 microg/kg) did not change the inhibition of DA release induced by apomorphine (100 microg/kg), whereas it prevented the reduction of DOPAC outflow induced by apomorphine (100 microg/kg). Administration of SB 206553, a selective blocker of 5-HT2C/2B receptors, dose-dependently increased DA outflow. The dose of 2.5 mg/kg SB 206553 caused a linear increase of DA output which reached a peak (+75%) 40 min after injection, while 1 mg/kg induced a more gradual increase of DA release which peaked (+54%) 60 min after administration of the drug. Treatment with RP 62203, a selective 5-HT2A receptor antagonist, did not produce any significant effect on DA outflow. Administration of ritanserin, a mixed 5-HT2A/2C receptor antagonist, did not cause any significant change of DA and DOPAC outflow. Taken together, these data indicate that selective blockade of 5-HT2/2B receptor subtypes increases DA release in the rat nucleus accumbens.     

Influence of total surface area of core material on yield of deposited coacervate

The neuronal cytoskeleton is one of the most profoundly altered organelles in late life neuro-degenerative disorders that are characterized by progressive impairments in cognitive abilities. The elucidation of the protein building blocks of these organelles as well as advances in understanding how these proteins become altered in Alzheimer's disease (AD) and other less common dementing illnesses, i.e., diffuse Lewy body disease (DLBD) or the Lewy body variant of AD (LBVAD), will provide insights into the molecular basis of these disorders. Within, we review evidence that normal adult human tau is abnormally phosphorylated and converted into the subunits of AD paired helical filaments (PHFs), and that Lewy bodies (LBs) represent accumulation of altered neurofilament (NF) triplet subunits. Although the precise biological consequences of PHF and LB formation in neurons is unknown, growing evidence suggests that the formation of PHFs and LBs from normal neuronal cytoskeletal proteins could have deleterious effects on neuronal function and survival. Finally, insights into the composition of PHFs and LBs could lead to the development of novel strategies for the timely and accurate diagnosis of AD, DLBD and the LBVAD.     

Turbulent flow in an intake-manifold

Three-dimensional, turbulent fluid flow analysis with computational methods has emerged as a viable tool in the design process of engine components for passenger cars. If applied in the early stages of the component development such analysis can help reduce the product development time drastically, and may support the design engineer to evaluate several competing design aspects before product completion. However the grid generation for complex geometries still poses a difficult and time consuming taks which strongly influences the accuracy of the numerical solution. The present paper studies the numerical solution of the flow in an inlet-manifold of a five-cylinder engine with two intake-ports for each cylinder. A fast and reliable grid generation technique is discussed in detail. The finite volume based Navier-Stokes solver with schemes of first and second order accuracy for the convective terms is used. Numerical results at several mass flow rates and different boundary conditions at the intake ports are compared with measurements. Finally, the computational results are discussed with respect to their applicability to support the design process of an inlet-manifold.     

An integrated microfluidic system for reaction, high-sensitivity detection, and sorting of fluorescent cells and particles

Presented is a novel approach for an integrated micro total analysis system (microTAS) based on a microfluidic on-chip device that supports ultrasensitive confocal detection of fluorescent cells and particles and subsequently allows for their precise sorting in the fluid phase with respect to spectroscopic properties, such as brightness and color. The hybrid silicone elastomer/glass chip first comprises a branched channel system to initiate fluid mixing and to hydrodynamically focus the sample solution down to a thin flow layer, matching the size of the confocal detection volume placed at that position and, thus, providing a high detection efficiency. In the subsequent on-chip module, the dispersed cells or particles can be sorted into two different output channels. The sorting process is realized by a perpendicular deflection stream that can be switched electrokinetically. The performance of the automated sorting routine is demonstrated by precise partition of a mixture of differently colored fluorescent beads. Moreover, the specifically branched channel geometry allows for direct implementation of reaction steps prior to detection and sorting, which is demonstrated by inducing a selective recognition reaction between the fluorescent protein R-phycoerythrin and a mixture of live bacterial cells exhibiting or lacking the respective surface antigens.     

Useful formula for moment computation of normal random variables with nonzero means

It is well known that higher order moments of normal random variables (RV) with zero means can be expressed by terms of second-order moments. In this correspondence an extension will be given for moments of normal RV with nonzero means.     

Computer study of bubble motion in a rotating liquid

The motion of a small, shape-preserving gas bubble through a rotating, homogeneous, incompressible, viscous liquid is investigated numerically be means of the Runge-Kutta-Nyström method. The fluid (liquid-gas) system spins about a fixed horizontal axis with constant angular velocity. Computer solutions are compared with experimental observations, and numerical experiences concerning step widths and computer accuracy are described.