Concentric needle electrode duration measurement and uptake area

Motor unit action potentials (MUAPs) were recorded with a standard concentric needle electrode inserted into the right biceps brachii muscle with different angular orientations of the beveled recording surface to the muscle fibers. Contrary to the predictions from computer simulations, the MUAP duration remained constant during needle rotation. This finding is used to reexamine the previous assumptions regarding the concentric needle's spatial uptake recording territory and the implications with respect to MUAP duration measurements.     

InSAR validation based on GNSS measurements in Bucharest

This research aims to complex validate radar displacement products over Bucharest based on Global Navigation Satellite System or GNSS. Validation was represented by the comparison of the recent TerraSAR-X (TSX) displacement results with field GNSS data. For this purpose, 24 and 27 high-resolution images acquired from the TSX satellite between 2011 and 2014 were processed by Permanent Scatterer Inteferometry (PSI) and Small BASeline Subset (SBAS) – interferometric techniques using synthetic aperture radar (SAR) images. The images were selected from a larger pool of SAR images, after consulting weather databases, which helped eliminate the images acquired under adverse weather conditions. GNSS locations were cross-checked on high-resolution optical images and 21 ground points (including three permanent stations) were selected. All these points were (1) characterized by high coherence on radar images; (2) were on the ground; (3) were situated in large vacant spaces; and (4) far away from radar obstacles such as trees. A major challenge in achieving the research goal was to overcome the lack of permanent stations at the ground level within the area of Bucharest. Instead of permanent stations, our team designed and installed forced centring systems that remained stable over time, eliminating the instalment errors of GNSS receivers. The line-of-site (LOS) components derived from GNSS data have been compared with those of the SAR scatterers closest to the GNSS markers (never coinciding). For each location, the vertical reading was projected in the LOS of the SAR sensor. These readings were compared with the displacement values determined at the locations of permanent scatterers (PSs) within 100 m from the GNSS nodes, by eliminating all scatterers on buildings. The comparison has shown a good agreement.     

Fractional wavelet transform for the quantitative spectral resolution of the composite signals of the active compounds in a two-component mixture

Fractional calculus is a powerful tool that has been applied successfully for the analysis of the complex systems. One interesting example of a complex mixture is given by the multicomponent pharmaceutical samples having constant matrix content. The main aim of this study is to develop a new approach based on the combined use of the fractional wavelet transform (FWT) and the continuous wavelet transform (CWT) in order to quantify atorvastatin (ATO) and amlodipine (AML) in their mixtures without requiring a chemical pretreatment. In the first step, the absorption spectra of the compounds and their samples were processed by the FWT method. In the next step, the CWT approach was applied to the fractional wavelet spectra obtained in the above step. The aim of the application of FWT is data reduction corresponding to the spectra of compounds and their commercial samples. In the following step, the CWT was used for the quantitative resolution of the composite signals of the analyzed compounds. After method validation, the proposed signal processing methods based on the combined use of the FWT and the CWT were successfully applied to the resolution of the composite spectra for the quantitation of atorvastatin (ATO) and amlodipine (AML) in tablets.     

Catalytic Combustion of the Stoichiometric n-Butane/Air Mixture on Isothermally Heated Platinum Wire

The catalytic combustion of the stoichiometric n-butane–air mixture per se or diluted with N₂, on a platinum wire at different initial pressures (10–70 kPa) and temperatures (690–1,080 K) was studied. The chemical heat flow rate, dQ _r/dt, of the surface reaction was measured in isothermal and isobaric conditions and the overall kinetic parameters were evaluated for both steady state and initial transient catalytic combustion. At low total pressure (10 kPa), the temperature dependence of dQ _r/dt indicated a normal (Arrhenius) behavior for 690 < T < 900 K, while at higher temperatures, over 900 K, an anti-Arrhenius behavior was found. The obtained results are consistent with a diffusion-controlled process, accompanied by reactant depletion around the catalytic surface, at higher temperatures.     

Synthesis of a new oxazoline macromonomer for dispersion polymerization

We synthesized new macromonomers containing vinylsilane moiety by cationic ring-opening polymerization of 2-ethyl-2-oxazoline. Kinetic studies proved that initiation with vinylsilane derivative, bearing chloride counterion, is a fast process followed by a slow propagation and absence of termination. We used the dispersion polymerization of styrene as test for the stabilizing efficiency of the newly synthesized macromonomer. The dispersion polymerization tests allowed the investigation of the effect of the macromonomer concentration on the number average diameter of the prepared polystyrene microspheres and its polydispersity index. In addition, we compared the stabilizing efficiency of commercial poly(N-vinylpyrollidone) with that of our macromonomer. Copolymerization of styrene with 10 wt% oxazoline macromonomer yielded stable and monodisperse microspheres, having the number average diameter of 1.8 μm and a good size polydispersity index of 1.05.     

Heterogeneous cationic polymerization of 1,3-dioxolane and 1,3-dioxepane using grafted BF3 on silica

Summary A BF₃ complex has been grafted onto silica and used as initiator to polymerize 1,3-dioxolane and 1,3-dioxepane. Grafting the active centers leads to a decrease of the reaction rate. The molecular weight increases steadily, parallel to the monomer conversion and the polydispersity is rapidly stabilized at a low value. MALDI-TOF mass spectrometry analysis shows that most of the polymer chains are initiated by a proton and lost a CH₂O unit. An exchange process between polymer chains bound to the silica and those remaining in the reactional medium was proposed. Finally, a semi-continuous process was investigated by adding continuously dioxolane during a 100 hours period. Received: 21 February 2001/ Revised version: 2 November 2001/ Accepted: 6 November 2001     

Enhanced Bio-Electrochemical Reduction of Carbon Dioxide by Using Neutral Red as a Redox Mediator

Microbial electrosynthetic cells containing Methylobacterium extorquens were studied for the reduction of CO₂ to formate by direct electron injection and redox mediator-assisted approaches, with CO₂ as the sole carbon source. The formation of a biofilm on a carbon felt (CF) electrode was achieved while applying a constant potential of −0.75 V versus Ag/AgCl under CO₂-saturated conditions. During the biofilm growth period, continuous H₂ evolution was observed. The long-term performance for CO₂ reduction of the biofilm with and without neutral red as a redox mediator was studied by an applied potential of −0.75 V versus Ag/AgCl. The neutral red was introduced into the systems in two different ways: homogeneous (dissolved in solution) and heterogeneous (electropolymerized onto the working electrode). The heterogeneous approach was investigated in the microbial system, for the first time, where the CF working electrode was coated with poly(neutral red) by the oxidative electropolymerization thereof. The formation of poly(neutral red) was characterized by spectroscopic techniques. During long-term electrolysis up to 17 weeks, the formation of formate was observed continuously with an average Faradaic efficiency of 4 %. With the contribution of neutral red, higher formate accumulation was observed. Moreover, the microbial electrosynthetic cell was characterized by means of electrochemical impedance spectroscopy to obtain more information on the CO₂ reduction mechanism.     

Glycine fluoromethylketones as SENP-specific activity based probes

We report here the synthesis and biochemical properties of a new peptidyl activity-based probe 1 for SUMO proteases, SENPs. The activity-based probe has at its C terminus a glycine-derived fluoromethylketone moiety as a reactive group designed to target the active-site cysteine of SENPs. Based on a study of the interactions between SENPs and SUMOs, we introduced further design elements that allow the activity-based probe to selectively target SENPs at low micromolar to high nanomolar concentrations. Moreover, 1 out-competes SUMO1 from the reversible SUMO1-SENP1 complex, thus suggesting that 1 and SUMO1 share a common binding site on SENP1.     

Statistical and Hybrid Methods for Speech Recognition in Romanian

The present paper describes the evolution of our work concerning the problem of speech recognition. Beginning with a classical hidden Markov model (HMM), we have investigated two ways to improve the performance of this basic structure. The first way was to realize a neuro-statistical hybrid by integrating a multilayer perceptron (MLP) as a posteriori probability estimator. The system was further refined by adding supplementary discriminative training (DT) based on the minimum classification error (MCE). Tests performed on a 15,000 isolated spoken-word database, showed an increase in the recognition rate from 92.2% for the HMM-based recognition system, to 94.7% for the HMM-MLP system, and then to 98.1% for the refined HMM-MLP-DT system. The second way to improve the classical HMM was to build a fuzzy-statistical hybrid, FHMM, based on a fuzzy similarity measure instead of the probabilistic measure specific to the usual statistical model. The benefits of the fuzzy measure introduction were evaluated on a vowel recognition task, and a decrease of approximately 3% in the error rate is reported.