Enzymatischer Nachweis von Carbamat-Pesticiden auf impr?gnierten Cellulose-Platten

Zusammenfassung Ein Nachweis für Carbamat-Pesticide mittels Enzym-Inhibition auf imprägnierten Cellulose-Schichten wurde ausgearbeitet. Bei Celluloseschichten, die mit Äthylglykol, Triäthylglykol und,-Ogydipropionitril imprägniert waren, erwies sich die Kombination Schweineleberesterase/Indophenylacetat (als chromogenes Substrat) als besonders empfindlicher Nachweis. Die Nachweisempfindlichkeit wurde in Abhängigkeit von der Art des Enzympräparates, des Substrates, des pH, der Inhibitionszeit und -temperatur, der Schichtdicke und des Impriignierungsmittels untersucht.

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Enzymatic detection of carbamate pesticides on impregnated cellulose plates

Summary The detection of carbamate pesticides by an enzyme-inhibition method on impregnated (ethylene glycol, triethylene glycol,,-ogydipropionitrile) cellulose thin layer plates is described. The enzyme-substrate combination: pig liver esterase-indophenylacetate (chromogenic substrate) was the most sensitive. The following parameters concerning the sensitivity of the method were investigated: enzyme source, substrate, pH, inhibition time, inhibition temperature, thickness of the cellulose layer, stationary phase.

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Herrn o.Univ.-Prof. Dr. O. Hoffmann-Ostenhof, Vorstand des Instituts für Allgemeine Biochemie, Universität Wien, zum 60. Geburtstag gewidmet.

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Wir danken dem Fonds zur Förderung der wissenschaftlichen Forschung, der uns die Durchführung dieser Arbeit (Projekt Nr. 2161) ermöglichte.

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Ing. B. Rapié dankt der SEA-Stiftung (Scientific Exchange Agreement) für die Gewährung eines Stipendiums.     

Peanut proteins as food supplements: A compositional study of selected Virginia and Spanish peanuts

Three peanut cultivars (Virginia, red-skinned, and white-skinned Spanish) were analyzed and compared as potential protein supplements for food uses. The seeds were solvent-extracted in the laboratory to yield defatted flours with 9–10% nitrogen contents. Protein isolates were prepared from the flours by subsequent extraction with dilute salt solutions buffered at pH 7.0. Various parameters were compared, such as total protein contents, soluble proteins, amino acid compositions of flours and protein isolates, free amino acids and free sugars of defatted flours, and certain trace minerals in flours and soluble proteins. The application of these results to the selection of certain types of peanuts for potential uses as protein supplements in food products is discussed. Presented in the Symposium, Oilseed Proteins, 66th Annual AOCS Meeting, Dallas, Texas, April 27–30, 1975.     

Some speculations concerning the selection of clinical psychologists.

The author makes the point that clinical work and scientific research require different intellectual orientations on the part of the psychologist. The ability to be effective and feel comfortable when assuming either attitude is a trait to look for in the selection of clinical psychologists. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Watershed and River Systems Management Program: Overview of Capabilities

Beginning in January 1992, the Bureau of Reclamation (Reclamation) and the US Geological Survey (USGS) formulated plans for the Watershed and River Systems Management Program—a cooperative interagency effort to develop and implement flexible and robust river basin management tools for the benefit of managers and decision makers using a data centered approach. In addition to Reclamation and the USGS, a number of other agencies and universities have made substantial contributions to the success of the program. The result has been the need driven research and development of state-of-the-art technology which benefits water managers and technical specialists in many river basins.     

Schulze and ranked-pairs voting are fixed-parameter tractable to bribe,manipulate, and control

Schulze and ranked-pairs elections have received much attention recently, and the former has quickly become a quite widely used election system. For many cases these systems have been proven resistant to bribery, control, or manipulation, with ranked pairs being particularly praised for being NP-hard for all three of those. Nonetheless, the present paper shows that with respect to the number of candidates, Schulze and ranked-pairs elections are fixed-parameter tractable to bribe, control, and manipulate: we obtain uniform, polynomial-time algorithms whose running times’ degrees do not depend on the number of candidates. We also provide such algorithms for some weighted variants of these problems.     

Algorithms and complexity analysis for some flow problems

Several network-flow problems with additional constraints are considered. They are all special cases of the linear-programming problem and are shown to be -complete. It is shown that the existence of a strongly polynomial-time algorithm for any of these problems implies the existence of such an algorithm for the general linear-programming problem. On the positive side, strongly polynomial algorithms for some parametric flow problems are given, when the number of parameters is fixed. These algorithms are applicable to constrained flow problems when the number of additional constraints is fixed.Work on the paper was done while at Stanford University and IBM Almaden Research Center. This research was partially supported by NSF PYI Grant CCR-8858097.     

Robust Reductions

We continue the study of robust reductions initiated by Gavaldà and Balcázar. In particular, a 1991 paper of Gavaldà and Balcázar [7] claimed an optimal separation between the power of robust and nondeterministic strong reductions. Unfortunately, their proof is invalid. We re-establish their theorem. Generalizing robust reductions, we note that robustly strong reductions are built from two restrictions, robust underproductivity and robust overproductivity, both of which have been separately studied before in other contexts. By systematically analyzing the power of these reductions, we explore the extent to which each restriction weakens the power of reductions. We show that one of these reductions yields a new, strong form of the Karp—Lipton theorem. Received November 1997, and in final form March 1999.     

Food vacuole proteome of the malarial parasite Plasmodium falciparum

The Plasmodium falciparum food vacuole (FV) is a lysosome-like organelle where erythrocyte hemoglobin digestion occurs. It is a favorite target in the development of antimalarials. We have used a tandem mass spectrometry approach to investigate the proteome of an FV-enriched fraction and identified 116 proteins. The electron microscopy analysis and the Western blot data showed that the major component of the fraction was the FV and, as expected, the majority of previously known FV markers were recovered. Of particular interest, several proteins involved in vesicle-mediated trafficking were identified, which are likely to play a key role in FV biogenesis and/or FV protein trafficking. Recovery of parasite surface proteins lends support to the cytostomal pathway of hemoglobin ingestion as a FV trafficking route. We have identified 32 proteins described as hypothetical in the databases. This insight into FV protein content provides new clues towards understanding the biological function of this organelle in P. falciparum.