Inhibition of bacterial foodborne pathogens by the lactoperoxidase system in combination with monolaurin

The lactoperoxidase system (LPS) and monolaurin (ML) are potential natural antimicrobial agents for use in foods. The LPS is considered to have greatest activity against Gram-negative bacteria while ML is usually considered to have greatest activity against Gram-positive bacteria. An LPS-ML combination system (utilizing lactoperoxidase (LPX) in the range 5-200 mg kg(-1) and ML in the range 50-1,000 ppm) inhibited growth of Escherichia coli O157:H7 and Staphylococcus aureus. Growth of S. aureus was inhibited more strongly in broth than in milk, in milk than in ground beef A similar pattern was observed for E. coli O157:H7, though enhanced inhibition by LPS-ML systems over that obtained in comparable LPS only systems was not observed in ground beef The inhibitory action of the LPS in combination with other lipids was also examined, with progressively weaker inhibition observed in combinations including palmitoleic acid, monopalmitolein, lauric acid, caprylic acid, and sodium lauryl sulphate.     

Competition of thermally injured listeria monocytogenes with a mesophilic lactic acid starter culture in milk for various heat treatments

Overnight tryptose broth cultures of three L monocytogenes strains were combined, centrifuged, suspended in 200 ml of tryptose phosphate broth, and heated at 56 degrees C for 20 min and at 64 degrees C for 2 min to obtain low-heat-injured (LHI) and high-heat-injured (HHI) cells, respectively, showing >99.6% injury. Flasks containing 200 ml of raw, low-heat-treated (56 degrees C for 20 min), high-heat-treated (64 degrees C for 2 min), pasteurized, and ultrahigh-temperature (UHT) milk were tempered to 31.1 degrees C and inoculated to contain 10(4) to 10(6) CFU/ml of LHI, HHI, or healthy L. monocytogenes cells and a commercial Lactococcus lactis subsp. lactis-Lactococcus lactis subsp. cremoris starter culture at levels of 0.5, 1.0, and 2.0%. Numbers of healthy and injured L. monocytogenes cells and starter organisms were determined using tryptose phosphate agar with or without 4.0% NaCl at selected intervals during 24 h of incubation at 31.1 degrees C. The presence of L. monocytogenes did not adversely affect the growth of the starter culture at any inoculation level. Overall, L. monocytogenes survived the 24-h fermentation period and grew to some extent. In starter-free controls. 76 to 81% of LHI cells and 59 to 69% of HHI cells were repaired after 8 h of incubation, with the lowest repair rates being observed for raw rather than heat-treated or pasteurized milk. Increased injury was observed for healthy L. monocytogenes cells at the 1.0 and 2.0% starter levels, with less injury seen for LHI and HHI cells. Raw and subpasteurized milk allowed less of a decrease in the percentage of injury and also showed higher numbers of injured cells than did pasteurized and UHT milks. These findings may have important implications for the survival of Listeria spp. in certain cheeses that can be prepared from raw or heat-treated milk.     

Deficits on the continuous performance test within the schizophrenia spectrum and the mediating effects of family history of schizophrenia.

Individuals with schizophrenia spectrum personality disorders (SSPD) and schizophrenia show similar cognitive impairments. The authors examined the contributions of SSPD symptoms and familial risk for schizophrenia to impairments on the Continuous Performance Test-Identical Pairs Version. Participants included 103 schizophrenia patients, 66 first-degree relatives (29 SSPD), and 103 community controls (26 SSPD) screened for family history of psychosis. Patients and SSPD relatives performed significantly worse than non-SSPD relatives and SSPD and non-SSPD community controls. No differences in performance were observed among non-SSPD relatives and SSPD and non-SSPD community controls. Results suggest a continuum in which risk for schizophrenia-related cognitive impairments is highest among patients and SSPD relatives, intermediate among non-SSPD relatives, and lowest among SSPD and non-SSPD community controls. Results suggest that SSPD in the absence of a family history of psychosis may be a phenocopy. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Intensities of E. coli nucleic acid Raman spectra excited selectively from whole cells with 251-nm light

Escherichia coli bacteria in the logarithmic growth phase have been investigated by UV resonance Raman spectroscopy. Bacterial whole-cell Raman spectra excited at 251 nm reflect nearly exclusively the nucleic acid composition even though a very large fraction of the bacterial mass is composed of protein. It has been demonstrated that if bacteria are grown under controlled (logarithmic growth) conditions, which give rise to organisms of known average biochemical composition, the intensities of E. coli Raman spectra can be explained quantitatively from the knowledge of component nucleic acid base resonance Raman cross sections.     

Respiratory motion of the heart from free breathing coronary angiograms

Respiratory motion compensation for cardiac imaging requires knowledge of the heart's motion and deformation during breathing. This paper presents a method for measuring the natural tidal respiratory motion of the heart from free breathing coronary angiograms. A three-dimensional (3-D) deformation field describing the cardiac and respiratory motion of the coronary arteries is recovered from a biplane acquisition. A cardiac respiratory parametric model is formulated and used to decompose the deformation field into cardiac and respiratory components. Angiograms from ten patients were analyzed. A 3-D translation motion model was sufficient for describing the motion of the heart in only two patients. For all patients, the heart translated caudally (mean, 4.9+/-1.9 mm; range, 2.4 to 8.0 mm) and underwent a cranio-dorsal rotation (mean, 1.5 degrees+/-0.9 degrees; range, 0.2 degrees to 3.5 degrees) during inspiration. In eight patients, the heart also translated anteriorly (mean, 1.3+/-1.8 mm; range, -0.4 to 5.1 mm) and rotated in a caudo-dextral direction (mean, 1.2 degrees+/-1.3 degrees; range, -1.9 degrees to 3.2 degrees).     

Alloyed Branch History: Combining Global and Local Branch History for Robust Performance

This paper introduces alloyed prediction, a new hardware-based two-level branch predictor organization that combines global and local history in the same structure, combining the advantages of current two-level predictors with those of hybrid predictors. The alloyed organization is motivated by measurements showing that wrong-history mispredictions are even more important than conflict-induced mispredictions. Wrong-history mispredictions arise because current two-level, history-based predictors provide only global or only local history. The contribution of wrong history to the overall misprediction rate is substantial because most programs have some branches that require global history and others that require local history. This paper explores several ways to implement alloyed prediction, including the previously proposed bi-mode organization. Simulations show that mshare is the best alloyed organization among those we examine, and that mshare gives reliably good prediction compared to bimodal (two-bit), two-level, and hybrid predictors. The robust performance of alloying across a range of predictor sizes stems from its ability to attack wrong-history mispredictions at even very small sizes without subdividing the branch prediction hardware into smaller and less effective components.     

Neonatal hind-paw injury disrupts acquisition of an instrumental response in adult spinal rats.

The present study was designed to evaluate the impact of neonatal injury on adult spinal plasticity in rats. Subjects were randomly assigned to 1 of 4 experimental conditions: (a) hind-paw injury at Postnatal Day (PD) 2, (b) hind-paw injury at PD 5, (c) anesthesia exposure only on PD 2, or (d) anesthesia exposure only on PD 5. Subjects receiving a unilateral neonatal hind-paw injury showed decreased mechanical threshold (hyperalgesia) on the previously injured hind paw throughout development. This decrease in threshold survived spinal transection (at T2) at 12 weeks of age. Injured subjects also showed significant impairment in a spinal instrumental learning task performed by the previously injured hind paw. This disruption of learning indicates a disruption of spinal plasticity that may be due to induction of long-term changes in nociceptive processing within the spinal cord. (PsycINFO Database Record (c) 2010 APA, all rights reserved)