Erythrodiol, a natural triterpenoid from olives, has antiproliferative and apoptotic activity in HT-29 human adenocarcinoma cells

Erythrodiol is the precursor of pentacyclic triterpenic acids present in Olea Europaea. Although olive oil and some of its constituents are reported to have anticarcinogenic activities, erythrodiol has not been assessed in its cell biological functions in detail. We therefore determined its effects on cell growth and apoptosis in human colorectal carcinoma HT-29 cells. Proliferation, cytotoxicity, and apoptosis were measured by fluorescence-based techniques. Erythrodiol inhibited cell growth with an EC50 value of 48.8 +/- 3.7 microM without any cytotoxic effects in a concentration range up to 100 microM. However, exposure of cells for 24 h to 50, 100, and 150 microM erythrodiol increased caspase-3-like activity by 3.2-, 4.8-, and 5.2-fold over that in control cells. We here demonstrate for the first time that, in colon adenocarcinoma cells, erythrodiol exerts antiproliferative and proapoptotic activity.     

Bootstrap confidence intervals for the mode of the hazard function

In many applications of lifetime data analysis, it is important to perform inferences about the mode of the hazard function in situations of lifetime data modeling with unimodal hazard functions. For lifetime distributions where the mode of the hazard function can be analytically calculated, its maximum likelihood estimator is easily obtained from the invariance properties of the maximum likelihood estimators. From the asymptotical normality of the maximum likelihood estimators, confidence intervals can be obtained. However, these results might not be very accurate for small sample sizes and/or large proportion of censored observations. Considering the log-logistic distribution for the lifetime data with shape parameter beta>1, we present and compare the accuracy of asymptotical confidence intervals with two confidence intervals based on bootstrap simulation. The alternative methodology of confidence intervals for the mode of the log-logistic hazard function are illustrated in three numerical examples.     

Improving the detection limit of anion-selective electrodes: an iodide-selective membrane with a nanomolar detection limit

The lower detection limit and the selectivity behavior of anion-selective electrodes (ISEs) are improved by using optimized inner solutions and membrane compositions. With a membrane based on the recently described ionophore [9]mercuracarborand-3, a detection limit of 2 x 10(-9) M has been achieved for iodide. Nevertheless, the improvements are less pronounced than in the case of cation ISEs. This is mainly due to the fact that so far no anion ISE is known with the extremely high selectivities of cation ISEs. If the membrane does not contain an ionophore, leaching of the ion exchanger from the membrane into the sample is also a relevant limiting factor except for ion exchangers of very high lipophilicity.     

Amplitude,phase, and hybrid ternary modulation modes of a twisted-nematic liquid-crystal display at approximately 400 nm

Applicability of a commercial twisted-nematic liquid-crystal display is examined at approximately 400 nm. Different modulation modes predicted by Jones-matrix calculus are experimentally tested. High contrast amplitude modulation with negligible loss, high contrast and low loss hybrid ternary modulation, and 1.5pi continuous phase delay without intensity modulation and with low loss are presented. Simulation results of a 4f holographic system prove the usefulness of the high contrast for amplitude modulation, and the importance of pi phase difference between high transmission white levels in a hybrid ternary modulation.     

Correlation between infrared transmission spectra and the interface trap density of SiO2 films

This work is an attempt to estimate the electrical properties of SiO₂ thin films by recording and analyzing their infrared transmission spectra. In order to study a big variety of films having different infrared and electrical properties, we studied SiO₂ films prepared by low pressure chemical vapor deposition (LPCVD) from SiH₄ + O₂ mixtures at 425 °C and annealed at 750 °C and 950 °C for 30 min. In addition thermally grown gate quality SiO₂ films of similar thickness were studied in order to compare their infrared and electrical properties with the LPCVD oxides. It was found that all studied SiO₂ films have two groups of Si–O–Si bridges. The first group corresponds to bridges located in the bulk of the film and far away from the interfaces, the grain boundaries and defects and the second group corresponds to all other bridges located near the interfaces, the grain boundaries and defects. The relative population of the bulk over the boundary bridges was found equal to 0.60 for the LPCVD film after deposition and increased to 4.0 for the LPCVD films after annealing at 950 °C. Thermally grown SiO₂ films at 950 °C were found to have a relative population of Si–O–Si bridges equal to 3.9. The interface trap density of the LPCVD film after deposition was found equal to 5.47 × 10¹² eV⁻¹ cm⁻² and decreases to 6.50 × 10¹⁰ eV⁻¹ cm⁻² after annealing at 950 °C for 30 min. The interface trap density of the thermally grown film was found equal to 1.27 × 10¹¹ eV⁻¹ cm⁻² showing that films with similar Si–O–Si bridge populations calculated from the FTIR analysis have similar interface trap densities.     

Weathering of metapelites from the Quadrilátero Ferrífero mineral province,southeastern Brazil

Low-grade metamorphic rocks are known for their low mechanical strength and high weatherability. In the Quadrilátero Ferrífero, an important mineral province located in southeastern Brazil, this set of features results in frequent issues with mining and road slope instability, mainly associated with phyllites. Despite the significant iron ore production that occurs in this province, little is known about the geotechnical properties of the materials constituting the weathering front. Here, we report work aimed at investigating the changes in the basic properties of phyllites at four weathering grades. The methodology used included the field classification and mapping of four weathering grades in order to assess their spatial distribution on a road slope, as well as sampling for geological characterization by petrography and X-ray diffraction (XRD). In addition, the authors performed a physical characterization of all weathering grades, including index properties, mercury porosimetry, and strength data obtained with a Schmidt hammer both in the field and in the laboratory as well as using the point load test (PLT). Results showed the relevance of structural features such as discontinuities and metamorphic foliation observed in the weathering morphology in situ, with different weathering intensities occurring along the slope. Changes in the porosity and pore size distribution in the weathering front were nonlinear, as were resistance variations. The difficulty involved in performing the macroscopic identification of weathering grades due to the very fine texture of the rock as well as microstructural heterogeneities are likely causes of the observed variability in phyllite properties. This fundamental knowledge may aid the prediction of short-term and long-term scenarios for slope stability based on rock weatherability.

     

The influence of spruce wood heat treatment on its thermal stability and burning process

This paper deals with the assessment of the influence of heat treatment of Norway spruce wood (Picea abies L.) on its thermal stability and burning process. Three types of samples were used for the research. The first group was comprised of untreated samples. The second group was comprised of samples heat treated according to the ThermoWood—Thermo-S thermal programme (maximum temperature during heat treatment is 190 °C), and the third group was modified according to the ThermoWood—Thermo-D thermal programme (maximum temperature during heat treatment is 212 °C). The influence of heat treatment on the burning process was assessed based on the heat release rate, effective heat of combustion and the yield of carbon monoxide. The influence of heat treatment on the thermal stability was assessed based on the resistant residue weight. Heat release rate, effective heat of combustion, yield of carbon monoxide and resistant residue weight were determined with a cone calorimeter for different heat flux densities. The results obtained prove that the spruce wood heat treatment has an important influence on the decrease of the maximum heat release rate and the increase of resistant residue weight. The results obtained further prove that the heat treatment has only a modest influence on the increase of the carbon monoxide yield, and its influence on the effective heat of combustion significantly depends on the heat flux density.     

Alginate fibers as photocatalyst immobilizing agents applied in hybrid photocatalytic/ultrafiltration water treatment processes

Ca alginate polymer fibers were developed to effectively disperse and stabilize an efficient photocatalyst such as AEROXIDE^® TiO₂ P25 in their matrix. The biopolymer/TiO₂ fibers were prepared and tested either in the hydrogel non-porous form or in the highly porous aerogel form prepared by sc-CO₂ drying. Batch photocatalytic experiments showed that the porous, Ca alginate/TiO₂ fibers, exhibited high efficiency for the removal of methyl orange (MO) from polluted water. In addition, their high porosity and surface area led to high MO degradation rate which was faster than that observed not only for their non-porous analogs but also of the bulk P25 TiO₂ powder. Specifically, 90% removal for 20 μM MO was achieved within 220 min for the porous sc-CO₂ dried fibers while for their non-porous analogs at 325 min. The corresponding value (at 60 μM MO) for the porous sc-CO₂ dried fibers was 140 min over 240 min for the AEROXIDE^® TiO₂ P25 as documented in the literature. Furthermore the composite alginate/photocatalyst porous fibers were combined with TiO₂ membranes in a continuous flow, hybrid photocatalytic/ultrafiltration water treatment process that led to a three fold enhancement of the MO removal efficiency at 400 ml of 20 μM MO total treated volume and to dilution rather than condensation in the membrane retentate as commonly observed in filtration processes. Furthermore the permeability of the photocatalytic membrane was enhanced in the presence of the fibers by almost 20%. This performance is achieved with 26 cm² and 31 cm² of membrane and stabilized photocatalyst surfaces respectively and in this context there is plenty of room for the up-scaling of both membranes and fibers and the achievement of much higher water yields since the methods applied for the development of the involved materials (CVD and dry-wet phase inversion in a spinning set-up) are easily up-scalable and are not expected to add significant cost to the proposed water treatment process.     

Are Phosphatidylcholine and Lysophosphatidylcholine Body Levels Potentially Reliable Biomarkers in Obesity? A Review of Human Studies

Phosphatidylcholines (PCs) are the major components of biological membranes in animals and are a class of phospholipids that incorporate choline as a headgroup. Lysophosphatidylcholines (LPCs) are a class of lipid biomolecules derived from the cleavage of PCs, and are the main components of oxidized low-density lipoproteins (oxLDLs) that are involved in the pathogenesis of atherosclerosis. Since obesity is associated with a state of chronic low-grade inflammation, one can anticipate that the lipidomic profile changes in this context and both PCs and LPCs are gaining attention as hypothetically reliable biomarkers of obesity. Thus, a literature search is performed on PubMed, Latin American and Caribbean Health Science Literature (LILACS), and Excerpta Medica DataBASE (Embase) to obtain the findings of population studies to clarify this hypothesis. The search strategy resulted in a total of 2403 reports and 21 studies were included according to the eligibility criteria. Controversial data on the associations of PCs and LPCs with body mass index (BMI) and body fat parameters have been identified. There is an inverse relationship between BMI and most species of PCs, and a majority of studies exhibited negative associations between BMI and LPCs. Other findings regarding the differences between PCs and LPCs in obesity are presented, and the associated uncertainties are discussed in detail.