Ion channel mimetic micropore and nanotube membrane sensors

This paper describes synthetic micropore and nanotube membranes that mimic the function of a ligand-gated ion channel; that is, these membranes can be switched from an "off" state (no or low ion current through the membrane) to an "on" state (higher ion current) in response to the presence of a chemical stimulus. Ion channel mimics based on both microporous alumina and Au nanotube membranes were investigated. The off state was obtained by making the membranes hydrophobic by chemisorbing either a C18 silane (alumina membrane) or a C18 thiol (Au nanotube membrane). Water and electrolyte are forbidden from entering these very hydrophobic pores/nanotubes. The transition to the on state was induced by the partitioning of a hydrophobic ionic species (e.g., a drug or a surfactant) into the membrane. The membrane switches to the on state because at a sufficiently high concentration of this ionic analyte species, the pores/nanotubes flood with water and electrolyte. A pH-responsive membrane was also prepared by attaching a hydrophobic alkyl carboxylic acid silane to the alumina membrane.     

G 2 interpolation and blending on surfaces

We introduce a method for curvature-continuous (G ²) interpolation of an arbitrary sequence of points on a surface (implicit or parametric) with prescribed tangent and geodesic curvature at every point. The method can also be used forG ² blending of curves on surfaces. The interpolation/blending curve is the intersection curve of the given surface with a functional spline (implicit) surface. For the construction of blending curves, we derive the necessary formulas for the curvature of the surfaces. The intermediate results areG ² interpolation/blending methods in IR².     

DDr. Otto Gebauer

Ohne Zusammenfassung     

Isoelektrische Fokussierung und zweidimensionale Auftrennung einer wasserl?slichen Proteinfraktion von Hart- und Weichweizen aus Teigwarenprodukten

Zusammenfassung Die isoelektrische Fokussierung einer wasserlöslichen Proteinfraktion von Hart- und Weichweizen aus Teigwarenprodukten wird mit der bisher üblichen disk-elektrophoretischen Trennmethode verglichen. Die Elektrofokussierung liefert im Gegensatz zur Disk-Elektrophorese auch bei Anwesenheit von Ei-Proteinen sowie nach vorgängiger Hitzedenaturierung der Proteine zuverlässige und genaue Resultate. Im weiteren wird zur besseren Auftrennung und Charakterisierung der wasserlöslichen Proteinfraktion eine zweidimensionale Trennmethode (isoelektrische Fokussierung und anschließende Elektrophorese) beschrieben.

---

Isoelectric focusing and two-dimensional separation of watersoluble protein fraction of hard and soft wheat from macaroni

Summary The isoelectric focusing of water-soluble protein fraction of hard and soft wheat from macaroni are compared with the disc electrophoresic separation methods. Electrofocusing produced more trustworthy and exacter results in the presence of egg proteins as well for heat-denaturated proteins. Further is described a better separation and characterisation of a water-soluble protein fraction using a two-dimensional system involving isoelectric focusing in the first dimension followed by electrophoresis in the second.

     

Assessment and evaluation of the radiological situation in the late phase of a nuclear accident

An overview of existing approaches on assessing and evaluating the radiological situation in the late phase of a nuclear accident is given in this paper. Special attention is paid to the weak points of existing approaches and to problems to be solved in the future. Assessment of the radiological situation can be based on both monitoring data and model predictions. Approaches have been developed for many years in both categories and have meanwhile reached some kind of maturity and also operational applicability. Nevertheless, some areas exist where significant improvements could be achieved in the near future, e.g. by combining monitoring data and model predictions, by improving the modelling of urban areas or by improving existing radioecological models.     

COLLATE – A collaboratory supporting research on historic European films

In the COLLATE project, we aim to design and implement a Web-based collaboratory for archives, scientists, and end users working with digitized cultural material. Our example domain is the historic film documentation comprising digitized material about European films of the early 20th century. Designed as a content- and context-based knowledge working environment for distributed user groups, the COLLATE system supports both individual work and collaboration of domain experts who are analyzing, evaluating, indexing, and annotating material in the data repository. The system provides appropriate task-based interfaces for indexing and annotating. As a multifunctional means of in-depth analysis, annotations can be made individually but also collaboratively, for example in the form of annotation of annotations. Combining results from manual and automatic indexing procedures, elaborate content- and context-based information retrieval mechanisms can be applied.     

Supercomputing: What have we learned from the TOP500 Project?

Since 1993 we compile and publish twice a year a list of the most powerful supercomputers in the world. In this article we analyze some major trends in High-Performance Computing (HPC) based on the quantitative data gathered over the years in this TOP500 project (see www.top500.org for a complete access to all data). We start with an overview of the project, including the motivation and rationale behind it. We present the results of the TOP500 project as of November 2003 focusing on the changes over time with respect to the performance levels, the overall dynamics in this market, the manufacturers active in this market-segment, the architectures, and technologies used for these systems, and the major centers using such systems.