Aspects of nucleosomal positional flexibility and fluidity

Nucleosomes have been considered until recently to be stable and uniquely localized particles. We focus here on two properties of nucleosomes that are emerging as central attributes of their functions: mobility and multiplicity of localization. The biological relevance of these phenomena is based on the fact that chromatin functions depend on the relative stability of nucleosomes, on their covalent or conformational modifications, their dynamics, their localization, and the density of their distribution. In order to understand these complex behaviors both the structure of the nucleosome core particles and the informational rules governing their interaction with defined DNA sequences are here taken into consideration. The fact that nucleosomes solve the problem of how to locate a specific interaction site on a potentially infinite combination of sequences, with interactions recurring to a controlled level of informational ambiguity and stochasticity, is discussed. Nucleosomes have been shown to slide along DNA. This novel facet of their behavior and its implications in chromatin remodeling are reviewed.     

High power GaN‐HEMT SPDT switches for microwave applications

In this article, the design, fabrication, and on‐wafer test of X‐Band and 2–18 GHz wideband high‐power SPDT MMIC switches in AlGaN/GaN technology are presented. The switches have demonstrated state‐of‐the‐art performance and RF fabrication yield better than 65%. Linear and power measurements for different control voltages have been reported and an explanation of the dependence of the power performances on the control voltage is given. In particular, the X‐band switch exhibits a 0.4 dB compression level at 10 GHz when driven by a 38 dBm input signal. The wideband switch shows a compression level of 1 dB at an input drive higher than 38 dBm across the entire bandwidth. © 2009 Wiley Periodicals, Inc. Int J RF and Microwave CAE, 2009.     

Volumetric Curved Planar Reformation for virtual endoscopy

Curved Planar Reformation (CPR) has proved to be a practical and widely used tool for the visualization of curved tubular structures within the human body. It has been useful in medical procedures involving the examination of blood vessels and the spine. However, it is more difficult to use it for large, tubular, structures such as the trachea and the colon because abnormalities may be smaller relative to the size of the structure and may not have such distinct density and shape characteristics. Our new approach improves on this situation by using volume rendering for hollow regions and standard CPR for the surrounding tissue. This effectively combines gray scale contextual information with detailed color information from the area of interest. The approach is successfully used with each of the standard CPR types and the resulting images are promising as an alternative to virtual endoscopy. Because the CPR and the volume rendering are tightly coupled, the projection method used has a significant effect on properties of the volume renderer, such as distortion and isometry. We describe and compare the different CPR projection methods and how they affect the volume rendering process. A version of the algorithm is also presented which makes use of importance driven techniques; this ensures the users attention is always focused on the area of interest and also improves the speed of the algorithm.     

Soft component adaptation

Component adaptation is widely recognised to be one of the crucial problems in Component-Based Software Engineering (CBSE). We present here a formal methodology for the soft adaptation of components presenting mismatching interaction behaviours. The notions of access rights (associating components with the services they are allowed to use) and subservicing (providing alternative services in place of those requested by components lacking the required access rights) are exploited to feature a secure and flexible adaptation of third-party components.     

A current‐mode 1kHz/10kHz low‐voltage integrated analogue PLL for lock‐in portable applications

We present a low‐supply voltage (2V) low‐power consumption (500W) analogue phase‐locked loop (PLL), working at two low frequencies (1 and 10kHz), to be used in an integrated lock‐in amplifier. An externally settable control bit allows the switching operation between the two different frequencies. The circuit has been designed in a standard 0.6–m CMOS technology and differs from the standard analogue PLL architectures for the current mode implementation of both the loop filter and of the oscillator. Three different locked waveforms (sinusoidal, triangular, squared) can be obtained at the PLL output. Simulation results, obtained through the use of PSPICE and using accurate transistor models, will be proposed. The pull‐in ranges are about ±250Hz around 1 and ±1.3kHz around 10kHz, with pull‐in times of about 10 and 4ms, respectively. Copyright © 2003 John Wiley & Sons, Ltd.     

Influence of β‐nucleation on polymorphism and properties in random copolymers and terpolymers of propylene

The competition between α, β, and γ polymorphs has been studied in several random copolymers and terpolymers of propylene, by performing X‐ray diffraction and differential scanning calorimetry experiments. Formation of β‐crystals, achieved by the addition of a β‐nucleating agent, was found to be highly dependent on comonomer type and crystallization conditions. Additionally, the mechanical properties have been evaluated from stress‐strain, microhardness and dynamic‐mechanical thermal analyses. POLYM. ENG. SCI., 2012. © 2012 Society of Plastics Engineers     

Characterization and properties of blended cement matrices containing activated bamboo leaf wastes

The worldwide production of bamboo generates large volumes of leaf wastes, which are deposited in landfills or burned in an uncontrolled manner, with negative effects in the environment. The ash obtained by calcining of the bamboo leaf waste, shows good qualities as supplementary cementing material for the production of blended cements.The current paper shows a detailed scientific study of a Brazilian bamboo leaf ash (BLA) calcined at 600 °C in small scale condition, by using different techniques (XRF, XRD, SEM/EDX, FT–IR, TG/DTG) and technical study in order to analyse the behaviour of this ash in blended cements elaborated with 10% and 20% by mass of BLA. The results stated that this ash shows a very high pozzolanic activity, with a reaction rate constant K of the order of 10^?1/h and type I CSH gel was the main hydrated phase obtained from pozzolanic reaction. The BLA blended cements (10% and 20%) complied with the physical and mechanical requirements of the existing European standards.