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61.
Quantification of Protein‐Induced Membrane Remodeling Kinetics In Vitro with Lipid Multilayer Gratings
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Troy W. Lowry Plengchart Prommapan Aubrey Kusi‐Appiah Nicholas Vafai Ewa A. Bienkiewicz David H. Van Winkle Scott M. Stagg Steven Lenhert 《Small (Weinheim an der Bergstrasse, Germany)》2016,12(4):506-515
The dynamic self‐organization of lipids in biological systems is a highly regulated process that enables the compartmentalization of living systems at micro‐ and nanoscopic scales. Consequently, quantitative methods for assaying the kinetics of supramolecular remodeling such as vesicle formation from planar lipid bilayers or multilayers are needed to understand cellular self‐organization. Here, a new nanotechnology‐based method for quantitative measurements of lipid–protein interactions is presented and its suitability for quantifying the membrane binding, inflation, and budding activity of the membrane‐remodeling protein Sar1 is demonstrated. Lipid multilayer gratings are printed onto surfaces using nanointaglio and exposed to Sar1, resulting in the inflation of lipid multilayers into unilamellar structures, which can be observed in a label‐free manner by monitoring the diffracted light. Local variations in lipid multilayer volume on the surface is used to vary substrate availability in a microarray format. A quantitative model is developed that allows quantification of binding affinity (K D) and kinetics (kon and koff). Importantly, this assay is uniquely capable of quantifying membrane remodeling. Upon Sar1‐induced inflation of single bilayers from surface supported multilayers, the semicylindrical grating lines are observed to remodel into semispherical buds when a critical radius of curvature is reached. 相似文献
62.
This article presents the results of a survey on how people perceive the Klimkówka storage reservoir located on the Ropa River in the Polish Carpathians. The analysis focused on establishing the role of various factors which impact the assessment of the reservoir by the local community. Community perception of the reservoir was analyzed in relation to several aspects linked to the reservoir itself, e.g. the risk of dam failure, feeling of safety, pros and cons, and how accustomed people were to the reservoir. A crucial issue was to identify how the real threat of a flood, experienced by people living below the dam, affected the way people perceived the reservoir. As a result of the analysis, a hierarchy of factors which determine people's assessment of large hydraulic structures was established. The results can be applied to determine specific measures aimed at limiting negative community perception of water infrastructure. 相似文献
63.
Learning-based algorithms in scheduling 总被引:1,自引:1,他引:0
Ewa Dudek-Dyduch 《Journal of Intelligent Manufacturing》2000,11(2):135-143
The aim of the paper is to present a conception of intelligent learning-based algorithms for scheduling. A general knowledge based model of a vast class of discrete deterministic processes is given. The model is a basis for the method of the synthesis of intelligent, learning-based algorithms, that is described in the paper. The designing simulation experiments that use learning is also described. To illustrate the presented ideas, the scheduling algorithm for a special NP-hard problem is given. The significant feature of the problem is that the retooling time depends not only on a pair of jobs to be processed directly one after the other, but also on the subset of jobs already performed. The proof of the NP-hardness of the problem is also given in the paper. 相似文献
64.
Andrzej Huczko Hubert Lange Ewa Ca ko Hanna Grubek-Jaworska Pawe Droszcz 《Fullerenes, Nanotubes and Carbon Nanostructures》2001,9(2):251-254
To determine whether carbon nanotubes can induce any significant health hazards we applied methods routinely used in the pathophysiological testing of asbestos-induced disease to show that the soot with a high content of CNTs does not induce any abnormalities of pulmonary function or measurable inflammation in guinea pigs treated with carbon nanotubes. 相似文献
65.
Ewa Wckelgrd 《Solar Energy Materials & Solar Cells》1998,56(1):1
Black nickel coatings electroplated in a nickel- and sodium chlorine aqueous solution have been prepared and parameters in the process have been optimized to achieve optimal solar selectivity. The best result is a solar absorptance of 0.96 and a thermal emittance of 0.10, as also has been obtained previously for the same type of coating 1H(15N, αγ)12C nuclear resonance reaction (NRR) have contributed with new information about surface chemical composition, morphology and atomic composition in depth profile. These investigations reveal that the coating is porous and contains mainly metallic nickel at the substrate–coating interface and mainly nickel hydroxide at the front surface.The stability of the coating has been tested regarding high-temperature and condensation at high humidity. It has been found that the solar absorptance changes initially, during the first hour of exposure at high temperature, but will then stabilize. Condensation causes a more severe attack on the coating by cracking it. This is contradictory to the results from previous tests at high temperature and humidity in which the black nickel coating was found to be resistant 相似文献
66.
Wioletta Florkiewicz Dagmara Malina Klaudia Pluta Karolina Rudnicka Adrian Gajewski Ewa Olejnik Boena Tyliszczak Agnieszka SobczakKupiec 《IET nanobiotechnology / IET》2019,13(7):726
The study was focused on the phytochemicals‐mediated biosynthesis of silver nanoparticles using leaf extracts and infusions from Cynara scolymus. To identify the antioxidant activity and total phenolic content, the 1,1‐diphenyl‐1‐picrylhydrazyl and Folin–Ciocalteau methods were applied, respectively. The formation and stability of the reduced silver ions were monitored by UV–vis spectrophotometer. The particle sizes of the silver nanoparticles were characterised using the dynamic light scattering technique and scanning electron microscope. The phase composition of the obtained silver nanoparticles was characterised by X‐ray diffraction. The silver nanoparticles suspension, artichoke infusion, and silver ions were separately tested towards potential cytotoxicity and pro‐inflammatory effect using mouse fibroblasts and human monocytes cell line, respectively. The total phenolic content and antioxidant activity of ethanol extract and infusion were found significantly higher as compared to aqueous extract and infusion. The UV–visible spectrophotometric analysis revealed the presence of the characteristic absorption band of the Ag nanoparticles. Moreover, it was found that with the increasing volume of plant extract, the average size of particles was increased. Biocompatibility results evidently showed that silver nanoparticles do not induce monocyte activation, however in order to avoid their cytotoxicity suspension at a concentration <2 ppm should be applied.Inspec keywords: pharmaceuticals, health and safety, renewable materials, toxicology, organic compounds, antibacterial activity, X‐ray diffraction, nanomedicine, nanoparticles, nanofabrication, suspensions, ultraviolet spectra, visible spectra, scanning electron microscopy, silver, particle sizeOther keywords: phytochemicals‐mediated biosynthesis, antioxidant activity, total phenolic content, dynamic light scattering technique, silver nanoparticles suspension, scanning electron microscopy, Cynara scolymus, 1,1 diphenyl‐1‐picrylhydrazyl method, cytotoxicity, immune compatibility, leaf extracts, UV‐vis spectrophotometry, particle size, Folin‐Ciocalteau methods, phase composition, X‐ray diffraction, artichoke infusion, pro‐inflammatory effect, mouse fibroblasts, human monocytes cell line, Ag 相似文献
67.
Ewa Cieślik Teresa Leszczyńska Agnieszka Filipiak-Florkiewicz Elżbieta Sikora Paweł M. Pisulewski 《Food chemistry》2007,105(3):976-981
Effects of blanching, boiling and freezing of selected cruciferous vegetables (Brussels sprouts, white and green cauliflower, broccoli, and curly cale) on their glucosinolate (GLS) contents were determined. It was found that blanching and cooking of the vegetables led to considerable (P < 0.05) losses of total GLS, from 2.7 to 30.0% and from 35.3 to 72.4%, respectively. No systematic changes in total GLS were found in the vegetables that were blanched and frozen for 48 h. In addition, the highest concentration of cancer-protective compounds, such as aliphatic and indole GLS, were found in Brussels sprouts (sinigrin and glucobrassicin) and in broccoli (glucoraphanin). 相似文献
68.
Electrodeposited Ni/SiC composite coatings were obtained in a Watts-type bath. The effect of fine SiC particles on polarization curves of the cathodic reduction of nickel ions was discussed. The incorporation of the particles into the deposit with respect to current density and SiC concentration in the bath was tested. Cathodic current efficiencies were also calculated. Structure of as-plated and heat-treated Ni/SiC composites were examined by means of metallography observations as well as scanning and transmission electron microscopy methods. Two phase transformations in the temperatures range of 20-700 °C were found. For annealed samples, Ni2Si and Ni3Si2 phases were identified. Hardening of the Ni/SiC composites as a function of the particle content in the deposit and annealing temperature was determined by means of the microhardness testing method. 相似文献
69.
70.
Beata Olejnik Anna Jarz?b Ewa M. Kratz Mariusz Zimmer Andrzej Gamian Miros?awa Ferens-Sieczkowska 《International journal of molecular sciences》2015,16(7):14933-14950
The impact of seminal plasma components on the fertilization outcomes in humans is still under question. The increasing number of couples facing problems with conception raises the need for predictive biomarkers. Detailed understanding of the molecular mechanisms accompanying fertilization remains another challenge. Carbohydrate–protein recognition may be of key importance in this complex field. In this study, we analyzed the unique glycosylation pattern of seminal plasma proteins, the display of high-mannose and hybrid-type oligosaccharides, by means of their reactivity with mannose-specific Galanthus nivalis lectin. Normozoospermic infertile subjects presented decreased amounts of lectin-reactive glycoepitopes compared to fertile donors and infertile patients with abnormal semen parameters. Glycoproteins containing unveiled mannose were isolated in affinity chromatography, and 17 glycoproteins were identified in liquid chromatography-tandem mass spectrometry with electrospray ionization. The N-glycome of the isolated glycoproteins was examined in matrix-assisted laser desorption ionization mass spectrometry. Eleven out of 27 identified oligosaccharides expressed terminal mannose residues, responsible for lectin binding. We suggest that lowered content of high-mannose and hybrid type glycans in normozoospermic infertile patients may be associated with impaired sperm protection from preterm capacitation and should be considered in the search for new infertility markers. 相似文献