Characterization of corticotropin-releasing hormone (CRH) in human skin

We have confirmed the expression of CRH and CRH receptor type 1 genes in human skin, cultured HaCaT keratinocytes, squamous cell carcinoma, and melanoma cells. The size of CRH messenger ribonucleic acid (mRNA), estimated by Northern blot hybridization, was 1.5 kilobases. CRH peptide was identified by reverse phase high pressure liquid chromatography separation in both whole skin and cultured cells. Forskolin and dexamethasone at concentrations of 10 micromol/L stimulated and inhibited, respectively, CRH peptide production in squamous cell carcinoma and melanoma cells, but had no significant effect on the CRH mRNA level. In melanoma cells, stimulation of melanogenesis down-regulated CRH receptor type 1 mRNA expression, but was without effect on CRH mRNA production. We suggest that in human skin the CRH signaling system is both operative and under regulatory control.     

Faradaic charge corrected colouration efficiency measurements for electrochromic devices

We report a new technique for measuring colouration efficiency (CE) for electrochromic polymers and devices. Faradaic charge associated with redox activity leading to chromic change was extracted from the total charge flow during a device switch. Typically, Faradaic charge is extracted from the total charge by first measuring a blank device (i.e. no polymer deposited). This is used as the baseline and subtracted from subsequent measurements with the polymer deposited into the device. The method is open to inaccuracies if the surface area before and after polymer deposition is different or if the inherent capacitance of the device is altered by the addition of the polymer(s). The new technique does not suffer from this drawback as it dispenses with the need to measure blank devices. To investigate the utility of this method a poly(3,4-ethylenedioxthiophene) (PEDOT) and polyaniline (PANI) electrochromic cell was tested. CE measurements at an optical density change of 95% (λ = 555 nm) were recorded for the following three conditions: uncorrected, CE_UC = 388 cm²/C; background corrected, CE_BC = 391 cm²/C and; Faradaic corrected, CE_FC = 2173 cm²/C. The result highlights the fact that the CE value of chromic polymers or devices may be higher than what is currently being reported.     

The Importance of Immunological Disorders in the Pathogenesis of Lichen Sclerosus in Pediatric Patients: A Systematic Review

Lichen sclerosus (LS) is defined as a chronic mucocutaneous inflammatory disease with a localization predominantly to the anus and genitals (vulvar sclerosus (VLS)). Pediatric lichen sclerosus (LS) is a chronic inflammatory skin condition with predilection for the anogenital area that if untreated can lead to scarring. Vulvar LS is characterized by two peaks in incidence: it occurs in prepubertal girls and in postmenopausal women. To date, several mechanisms and risk factors have been proposed in the pathogenesis of pediatric vulvar LS; however, the etiology of this condition is still not fully understood and constitutes a challenge for scientists and clinicians. The presented research aimed to systematically review the existing literature on the pathogenesis of pediatric LS and to identify possible underlying autoimmune mechanisms and molecular networks. The clinical presentation of pediatric lichen sclerosus and available treatment modalities are also presented to acquaint a broader audience with this underdiagnosed and undertreated condition. As a result of our review, we discuss several potential mechanisms, molecules, and pathways that have been recognized in this disease. The purpose of our review was also to summarize what we can induce in further studies, which will ultimately help to identify the mechanism responsible for the disease and aid in the development of new, more effective treatment strategies for diagnosis and treatment by clinicians and researchers.     

Interactions of single-walled carbon nanotubes with monosaccharides

Closed-end single-walled carbon nanotubes were wetted in aqueous solutions of monosaccharides, forming weak surface complexes, as proven by the estimation of the content of monosaccharides in complexes isolated from aqueous solutions. The complexation was confirmed by micro-Raman spectroscopy. The Gaussian 03 (Molecular Mechanics UFF method) computations of total energy of the single-walled carbon nanotube-monosaccharides inclusion and surface complexes showed that inclusion complexes should be more stable than corresponding surface complexes. Computed total energies for particular complexes pointed to a lack of preferences for the formation of complexes with either alpha- or beta-tautomers and either pyranoses or furanoses. The forms preferred in the formation of the surface complexes usually differ from these favored in the formation of the inclusion complexes.     

The role of social media data in operations and production management

Social media data contain rich information in posts or comments written by customers. If those data can be extracted and analysed properly, companies can fully utilise this rich source of information. They can then convert the data to useful information or knowledge, which can help to formulate their business strategy. This cannot only facilitate marketing research in view of customer behaviour, but can also aid other management disciplines. Operations management (OM) research and practice with the objective to make decisions on product and process design is a fine example. Nevertheless, this line of thought is under-researched. In this connection, this paper explores the role of social media data in OM research. A structured approach is proposed, which involves the analysis of social media comments and a statistical cluster analysis to identify the interrelationships amongst important factors. A real-life example is employed to demonstrate the concept.     

Non-covalent functionalization of multi-walled carbon nanotubes with organic aromatic compounds

Several aromatic compounds derived from benzene by its annelation (naphthalene, anthracene, phenanthrene, pyrene) and exocyclic substitution (e.g., nitrobenzene, dinitrobenzenes, trinitrobenzene, chlorobenzene, N,N-dimethylaniline, and others) and endocyclic substitution (pyridine, quinoline, isoquinoline) efficiently sorbed on multi-walled carbon nanotubes (MWCNTs). Equilibrium constants for the sorption process have been determined. Computations of the energy of formation were performed for surface complexes of those arenes on single-walled carbon nanotubes (SWCNTs). Formation energies of those complexes were correlated against the experimental equilibrium constants. The latter were also correlated against calculated LUMO energy of the arenes. Solely, limited tendencies to the linearity could be observed. The analysis of the results of the correlations indicated that the arenes acting as the charge acceptors formed stronger complexes than arenes with a high electron density in the molecular orbital, for instance, N, N-dimethylaniline. The area of the arene--SWCNT contact was very essential for the complexation. The proximity of the HOMO orbital of SWCNT and LUMO of the arene was another essential factor. Bulky substituents in the arene molecules obscured their efficient contact with SWCNT.     

Cytochemical localization of acetylcholine receptors at the neuromuscular junction by means of horseradish peroxidase-labeled alpha-bungarotoxin

The cytochemical localization of acetylcholine (ACh) receptors at the neuromuscular junction was investigated with a procedure utilizing alpha-bungarotoxin (alpha-BtX) labeled directly with horseradish peroxidase (HRP). Following incubation of tissues in the conjugate and reaction for peroxidase, activity was observed on the junctional folds of the motor endplate. A uniform layer of reaction product approximately 15 nm thick occurred over the apical portions of the junctional folds. Membranes at the bases of the synaptic cleft showed only small amounts of reaction product. Non-junctional regions of the muscle fiber were unreactive. Activity was also observed in the membrane of the axon facing the muscle surface, often including the axolemma overlying the "active zones" of the nerve terminal. Such presynaptic activity was still evident on nerve terminals disjuncted from the synapse by enzymatic treatment prior to incubation in the conjugate. This localization indicates the possible presence of presynaptic ACh receptors within the axolemma. In muscle denervated for 7-12 days, motor endplates were reactive and parajunctional sarcolemma showed slight activity, but most extrajunctional regions contained no obvious accumulations of reaction product. Activity at all sites was prevented by preincubation of tissues in native alpha-BTX prior to incubation in the conjugate and reaction for HRP. This procedure represents a simple and convenient method for the high resolution localization of ACh receptors.     

Spatio‐temporal analysis and modeling of short‐term wind power forecast errors

Forecasts of wind power production are increasingly being used in various management tasks. So far, such forecasts and related uncertainty information have usually been generated individually for a given site of interest (either a wind farm or a group of wind farms), without properly accounting for the spatio‐temporal dependencies observed in the wind generation field. However, it is intuitively expected that, owing to the inertia of meteorological forecasting systems, a forecast error made at a given point in space and time will be related to forecast errors at other points in space in the following period. The existence of such underlying correlation patterns is demonstrated and analyzed in this paper, considering the case‐study of western Denmark. The effects of prevailing wind speed and direction on autocorrelation and cross‐correlation patterns are thoroughly described. For a flat terrain region of small size like western Denmark, significant correlation between the various zones is observed for time delays up to 5 h. Wind direction is shown to play a crucial role, while the effect of wind speed is more complex. Nonlinear models permitting capture of the interdependence structure of wind power forecast errors are proposed, and their ability to mimic this structure is discussed. The best performing model is shown to explain 54% of the variations of the forecast errors observed for the individual forecasts used today. Even though focus is on 1‐h‐ahead forecast errors and on western Denmark only, the methodology proposed may be similarly tested on the cases of further look‐ahead times, larger areas, or more complex topographies. Such generalization may not be straightforward. While the results presented here comprise a first step only, the revealed error propagation principles may be seen as a basis for future related work. Copyright © 2010 John Wiley & Sons, Ltd.