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81.
Martina Fabris Fabio Del Ben Emanuela Sozio Antonio Paolo Beltrami Adriana Cif Giacomo Bertolino Federica Caponnetto Marco Cotrufo Carlo Tascini Francesco Curcio 《International journal of molecular sciences》2022,23(9)
The main aim of this study was to identify the most relevant cytokines which, when assessed in the earliest stages from hospital admission, may help to select COVID-19 patients with worse prognosis. A retrospective observational study was conducted in 415 COVID-19 patients (272 males; mean age 68 ± 14 years) hospitalized between May 2020 and March 2021. Within the first 72 h from hospital admission, patients were tested for a large panel of biomarkers, including C-reactive protein (CRP), Mid-regional proadrenomedullin (MR-proADM), Interferon-γ, interleukin 6 (IL-6), IL-1β, IL-8, IL-10, soluble IL2-receptor-α (sIL2Rα), IP10 and TNFα. Extensive statistical analyses were performed (correlations, t-tests, ranking tests and tree modeling). The mortality rate was 65/415 (15.7%) and a negative outcome (death and/or orotracheal intubation) affected 98/415 (23.6%) of cases. Univariate tests showed the majority of biomarkers increased in severe patients, but ranking tests helped to select the best variables to put on decisional tree modeling which identified IL-6 as the first dichotomic marker with a cut-off of 114 pg/mL. Then, a good synergy was found between IL-10, MR-proADM, sIL2Rα, IP10 and CRP in increasing the predictive value in classifying patients at risk or not for a negative outcome. In conclusion, beside IL-6, a panel of other cytokines representing the degree of immunoparalysis and the anti-inflammatory response (IP10, sIL2Rα and IL-10) showed synergic role when combined to biomarkers of systemic inflammation and endothelial dysfunction (CRP, MR-proADM) and may also better explain disease pathogenesis and suggests targeted intervention. 相似文献
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Reginaldo Muccillo Daniel Zanetti de Florio Fabio C. Fonseca Sabrina G. M. Carvalho Eliana N. S. Muccillo 《International Journal of Applied Ceramic Technology》2022,19(2):906-912
Cosintering (La0.84Sr0.16MnO3 thin-film cathode/ZrO2: 8 mol% Y2O3 thin-film solid electrolyte/55 vol.% ZrO2:8 mol% Y2O3 + 45 vol.% NiO anode, ϕ = 12 × 1.5 mm thick pellet) was achieved by applying an electric field for 5 min at 1200°C. Impedance spectroscopy measurements of the anode-supported three-layer cell show an improvement of the electrical conductivity in comparison to that of a conventionally sintered cell. The scanning electron microscopy images of the cross-sections of electric field-assisted pressureless sintered cells show a fairly dense electrolyte and porous anode and cathode. Joule heating, resulting from the electric current due to the application of the AC electric field, is suggested as responsible for sintering. Dilatometric shrinkage curves, electric voltage and current profiles, impedance spectroscopy diagrams, and scanning electron microscopy micrographs show how anode-electrolyte-cathode ceramic cells can be cosintered at temperatures lower than the usually required. 相似文献
84.
Danilo Marimpietri Irma Airoldi Angelo Corso Faini Fabio Malavasi Fabio Morandi 《International journal of molecular sciences》2021,22(8)
The long-underestimated role of extracellular vesicles in cancer is now reconsidered worldwide by basic and clinical scientists, who recently highlighted novel and crucial activities of these moieties. Extracellular vesicles are now considered as king transporters of specific cargoes, including molecular components of parent cells, thus mediating a wide variety of cellular activities both in normal and neoplastic tissues. Here, we discuss the multifunctional activities and underlying mechanisms of extracellular vesicles in neuroblastoma, the most frequent common extra-cranial tumor in childhood. The ability of extracellular vesicles to cross-talk with different cells in the tumor microenvironment and to modulate an anti-tumor immune response, tumorigenesis, tumor growth, metastasis and drug resistance will be pinpointed in detail. The results obtained on the role of extracellular vesicles may represent a panel of suggestions potentially useful in practice, due to their involvement in the response to chemotherapy, and, moreover, their ability to predict resistance to standard therapies—all issues of clinical relevance. 相似文献
85.
Jessica Da Gama Duarte Luke T. Quigley Anna Rachel Young Masaru Hayashi Mariko Miyazawa Alex Lopata Nunzio Mancuso Mikio Mikami Andreas Behren Els Meeusen 《International journal of molecular sciences》2021,22(20)
Ovarian cancers include several disease subtypes and patients often present with advanced metastatic disease and a poor prognosis. New biomarkers for early diagnosis and targeted therapy are, therefore, urgently required. This study uses antibodies produced locally in tumor-draining lymph nodes (ASC probes) of individual ovarian cancer patients to screen two separate protein microarray platforms and identify cognate tumor antigens. The resulting antigen profiles were unique for each individual cancer patient and were used to generate a 50-antigen custom microarray. Serum from a separate cohort of ovarian cancer patients encompassing four disease subtypes was screened on the custom array and we identified 28.8% of all ovarian cancers, with a higher sensitivity for mucinous (50.0%) and serous (40.0%) subtypes. Combining local and circulating antibodies with high-density protein microarrays can identify novel, patient-specific tumor-associated antigens that may have diagnostic, prognostic or therapeutic uses in ovarian cancer. 相似文献
86.
Fabio Forghieri Giovanni Riva Ivana Lagreca Patrizia Barozzi Francesca Bettelli Ambra Paolini Vincenzo Nasillo Beatrice Lusenti Valeria Pioli Davide Giusti Andrea Gilioli Corrado Colasante Laura Galassi Hillary Catellani Francesca Donatelli Annalisa Talami Rossana Maffei Silvia Martinelli Leonardo Potenza Roberto Marasca Enrico Tagliafico Rossella Manfredini Tommaso Trenti Patrizia Comoli Mario Luppi 《International journal of molecular sciences》2021,22(17)
The C-terminal aminoacidic sequence from NPM1-mutated protein, absent in normal human tissues, may serve as a leukemia-specific antigen and can be considered an ideal target for NPM1-mutated acute myeloid leukemia (AML) immunotherapy. Different in silico instruments and in vitro/ex vivo immunological platforms have identified the most immunogenic epitopes from NPM1-mutated protein. Spontaneous development of endogenous NPM1-mutated-specific cytotoxic T cells has been observed in patients, potentially contributing to remission maintenance and prolonged survival. Genetically engineered T cells, namely CAR-T or TCR-transduced T cells, directed against NPM1-mutated peptides bound to HLA could prospectively represent a promising therapeutic approach. Although either adoptive or vaccine-based immunotherapies are unlikely to be highly effective in patients with full-blown leukemia, these strategies, potentially in combination with immune-checkpoint inhibitors, could be promising in maintaining remission or preemptively eradicating persistent measurable residual disease, mainly in patients ineligible for allogeneic hematopoietic stem cell transplant (HSCT). Alternatively, neoantigen-specific donor lymphocyte infusion derived from healthy donors and targeting NPM1-mutated protein to selectively elicit graft-versus-leukemia effect may represent an attractive option in subjects experiencing post-HSCT relapse. Future studies are warranted to further investigate dynamics of NPM1-mutated-specific immunity and explore whether novel individualized immunotherapies may have potential clinical utility in NPM1-mutated AML patients. 相似文献
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89.
Fabio Maria Sabbatini Dr. Romano Di Fabio Dr. Mauro Corsi Dr. Paolo Cavanni Dr. Steve M. Bromidge Dr. Yves St‐Denis Dr. Lucilla D'Adamo Dr. Marilisa Rinaldi Dr. Chiara Savoia Dr. Claudia Mundi Dr. Benedetta Perini Dr. Andrew J. Carpenter Dr. Giovanna Dal Forno Dr. Federico Faggioni Dr. Michela Tessari Dr. Francesca Pavone Dr. Alberto Buson Dr. Mario Mattioli Dr. Elisabetta Perdona' Dr. Sergio Melotto Dr. 《ChemMedChem》2010,5(9):1450-1455
90.
A single-step method to coat and bifunctionalize water-reduced gold nanoparticles (NPs) with two distinct reactive groups is reported. The coating is based on a peptide that bonds to the NPs surface by its N-cysteine amino acid, terminates with a C-terminal lysine, and stabilizes the colloids, thanks to the surface organization provided by the rest of the non-polar chain. The process yields stable, non-cytotoxic NPs presenting reactive amine and carboxylic groups on the surface; these allow rapid, selective and modular conjugation of virtually any chosen biomolecule or fluorophore. Functionalized and conjugated nanostructures are analyzed by electrophoresis, SEM, SERS; their biocompatibility and delivery capability are tested by cellular-uptake experiments. 相似文献