Alcohol alters men's perceptual and decisional processing of women's sexual interest.

The current investigation examines the etiology of men's errors in sexual perception after moderate alcohol use. Sensitivity and bias estimates, derived from multidimensional signal detection analysis, revealed that men's alcohol-influenced performance was associated with declining sensitivity to the distinction between women's friendliness and sexual interest. However, sensitivity to the distinction between conservative and provocative clothing was unaffected. Similarly, an alcohol dose led to an increased bias to respond that women's ambiguous cues were sexual interest (rather than friendliness) but did not influence response thresholds for clothing style. Thus, there was specificity to the perceptual and decisional changes associated with alcohol use rather than a simple degradation of men's capacity to process all dating-relevant cues in the environment. Given the link between alcohol use, sexual misperception, and acquaintance-initiated sexual coercion, understanding the etiology of sexual misperception in the context of alcohol use may inform sexual coercion prevention efforts. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Ab initio calculations on the five-membered alumino-silicate framework rings model: implications for dissolution in alkaline solutions

An ab initio restricted Hartree-Fock calculation utilising the standard 6-31G basis set was used to calculate total energies after PM3 calculations of energy-optimised geometries for the five-membered alumino-silicate framework rings cluster for a total of ten T sites. Calculations have shown that in the absence of protons or other ions, the most favourable sites for 1A1, 2A1 and 3A1 substitution of Si are the T6, T1 and T9 sites respectively. With more Al atoms replacing Si in a cluster, T-O bond lengths and T-O-T angles show lengthening and sharpening trends respectively, which indicates that the structure is distorted to a more relaxed symmetry with Obr atoms moving outwards. The calculated bond lengths and angles have been shown to match the values observed in previous studies, including those for a four-membered alumino-silicate single ring cluster. Based on the optimised five-membered alumino-silicate framework rings model, a further ab initio HF calculation has been conducted on ring breakage for releasing Al(Q3) and Si(Q3) centres to form T(OH)4 and HOT(OM)3 tetrahedra under local and highly alkaline environment. The obtained results suggest that Al(Q3) compared with Si(Q3) breaks more readily with the exothermal reaction enthalpy being in excess of -244.4 kJ/mol, while the most reactive Si(Q3) centre shows an exothermal reaction enthalpy of only -33.8 kJ/mol. This indicates that Al dissolves in preference to Si in local environment. The dissolution mechanism of the five-membered Al-Si framework rings model in highly alkaline solutions has been suggested to be composed of an ion-pairing reaction and an interaction between the remaining broken ring cluster triple bond TOH and MOH.     

Percutaneous bile duct drainage; experiences with a new type of endoprosthesis]

Self expandable stents were placed percutaneously in 105 patients with malignant biliary obstruction. Stent diameter was 1 cm; length, 3.5-10.5 cm. Of the 60 patients with common bile duct obstruction, 50 died 0.2-12 months (median 3 months) after stent insertion. Two patients developed recurrent jaundice and cholangitis after 6 and 12 months, respectively. One patient underwent reintervention. Ten patients, one after a successful reintervention, were alive without jaundice 1-8 months (median 5 months) after stent placement. Of the 45 patients with hilar lesions, 26 died 0.7-18 months (median 5 months) after stent placement, five of them with signs of cholangitis. Nineteen are alive 1-21 months (median 7 months) afterwards. Reinterventions were carried out in 13 patients (29%). The most common cause of stent malfunction was tumour overgrowth. Stent-related complications were seen in three patients.     

Proteolytic cleavage and activation of PAK2 during UV irradiation-induced apoptosis in A431 cells

Exposure of mammalian cells to ultraviolet (UV) light elicits a cellular response and can also lead to apoptotic cell death. In this report, we show that a 36-kDa myelin basic protein (MBP) kinase detected by an in-gel kinase assay can be dramatically activated during the early stages of UV irradiation-triggered apoptosis of A431 cells. Immunoblot analysis revealed that this 36-kDa MBP kinase could be recognized by an antibody against the C-terminal regions of a family of p21Cdc42/Rac-activated kinases (PAKs). By using this antibody and a PAK2-specific antibody against the N-terminal region of PAK2 as studying tools, we further demonstrated that UV irradiation caused cleavage of PAK2 to generate a 36-kDa C-terminal catalytic fragment and a 30-kDa N-terminal fragment in A431 cells. The appearance of the 36-kDa C-terminal catalytic fragment of PAK2 matched exactly with the activation of the 36-kDa MBP kinase in A431 cells upon UV irradiation. In addition, UV irradiation also led to activation of CPP32/caspase-3, but not ICH-1L/caspase-2 and ICE/caspase-1, in A431 cells and the kinetics of activation of CPP32/caspase-3 appeared to correlate well with that of DNA fragmentation and of cleavage/activation of PAK2, respectively. Moreover, blockage of activation of CPP32/caspase-3 by pretreating the cells with two specific tetrapeptidic inhibitors for caspases (Ac-DEVD-cho and Ac-YVAD-cmk) could significantly attenuate the extent of cleavage/activation of PAK2 induced by UV irradiation. Collectively, the results demonstrate that cleavage and activation of PAK2 can be induced during the early stages of UV irradiation-triggered apoptosis and indicate the involvement of CPP32/caspase-3 in this process.     

Separation of bacterial capsular and lipopolysaccharides by preparative electrophoresis

Preparative gel electrophoresis was used to separate and purify extracellular, capsular and lipopolysaccharides (EPSs, CPSs, and LPSs, respectively) from crude bacterial extracts. The procedure effectively separates CPS from LPSs. In addition discreet size ranges of these various polysaccharides can be isolated. The 'rough' (R-type), 'smooth' (S-type), and 'semi-smooth' LPSs were separated from one another. In addition different size classes of 'semi-smooth', or S-type LPS, can be separated. This procedure was demonstrated for diverse bacterial species, including the soil bacteria Rhizobium fredii, and the enteric bacterial species, Salmonella enteritidis and Proteus mirabilis. In the latter case, it was also possible to separate capsular polysaccharide from its lipid-bound form.     

Evidence for a cyclic GMP-independent mechanism in the anti-platelet action of S-nitrosoglutathione

Using a directed forgetting task, the authors tested in 2 experiments the hypothesis that repressors would be superior to controls in forgetting negative experimental material. Consistent with previous studies, there was an overall directed forgetting effect, with significantly more to-be-remembered material recalled than to-be-forgotten (TBF) material. In both experiments, repressors forgot more negatively valenced words in the TBF set than did nonrepressors, suggesting that repressors have an enhanced capability for using retrieval inhibition. The data offer preliminary support for a cognitive account of repressors' deficits in recalling negative autobiographical memories.     

Induction of neutralizing antibody in mice by immunization with recombinant 56 kDa protein of Orientia tsutsugamushi

Anti-oriential antibody inhibits Orientia tsutsugamushi attachment to, and penetration of, host cells. However, O. tsutsugamushi antigens that induce the production of a neutralizing antibody have not been identified. The authors immunized mice and rabbits with the recombinant 56 kDa protein of O. tsutsugamushi fused to the maltose binding protein of Escherichia coli (MBP-Bor56) and analysed their effect on O. tsutsugamushi attachment to or penetration of L929 cells. O. tsutsugamushi attachment and penetration were measured by using an indirect immunofluorescent antibody assay (IFA). O. tsutsugamushi growth in L929 cells was determined by [3H]thymidine uptake assay. By IFA, we observed a 96% reduction of attachment or penetration of O. tsutsugamushi treated with rabbit anti-MBP-Bor56 sera. [3H]thymidine uptake showed that mouse anti-MBP-Bor56 sera caused a 91% reduction in O. tsutsugamushi growth, when compared to mouse anti-MBP sera. These results suggest that the 56 kDa protein of O. tsutsugamushi plays an important role in O. tsutsugamushi attachment to or penetration of cells.