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81.
J.M. Fernandez Paris 《Cement and Concrete Research》1973,3(6):715-721
The author has utilised magnetic agitators to determine the “ferromagnetic fraction” of a number of varieties of portland cement. He has been able to establish, not without some surprise, that this “ferromagnetic fraction” constitutes a significant proportion of the cement. To determine their composition, X-ray difractograms have been obtained for each of the samples. The tests described in this paper seek to determine the magnetic fraction and obtain a method of quantifying it. 相似文献
82.
Chromosome complements of murine thymic lymphomas induced by an alkylating agent N-methyl-N-nitrosourea (MNUA) were analyzed microscopically and karyotypically using the Q-banding technique. The chemical carcinogen was injected intraperitoneally into either neonatal or 7-week-old CFW/D mice. In addition, thymic lymphomas induced in 7-week-old AKR mice and thymic lymphomas developed spontaneously in this strain were also examined. All six lymphomas induced in neonatal CFW/D had hyperdiploid cell lines that accounted for 90% of the cells analyzed. Chromosome analysis of lymphomas induced in adult DFW/D mice showed that only out of nine lymphomas had predominantly hyperdiploid cell lines. The remaining five lymphomas had diploid modal chromosome number although they also carried a variant line characterized by 41 chromosomes. All eight lymphomas induced in adult AKR mice and six out of seven spontaneous AKR lymphomas showed predominantly diploid modal line. The remaining spontaneous lymphoma had a hyperdiploid stem line of 41 chromosomes. Microscopic and karyotypic analysis further identified trisomy 15 as the regular chromosome abnormality in the hyperdiploid cells in lymphomas of each group, whereas cells with diploid chromosome number had no detectable chromosome abnormality. Additional trisomies were also found, but their appearance was restricted to individual tumors. Thus, the incidence of trisomy 15 in lymphomas induced by MNUA in adult CFW/D and AKR mice, as well as in the spontaneous AKR lymphomas, is significantly lower than that in lymphomas induced in neonatal mice by the same carcinogen. 相似文献
83.
84.
Althaus R Torres A Peris C Beltran MC Fernandez N Molina MP 《Journal of food protection》2003,66(3):473-478
The presence of drug residues in ewe's milk samples can be determined by microbial assays. The main limitation of these tests is the large number of false-positive results associated with them. False-positive results can be explained by the interaction of certain substances naturally existing in ewe's milk with the growth of the microorganism used in the test. In this study, milk chemical composition (fat, protein, lactose, total solids), somatic cell counts (SCCs), free fatty acid concentrations, and lactoperoxidase system components were determined in order to investigate their influence on the rate of false-positive results for the BRT and Delvotest microbiological inhibitor tests. Milk samples were obtained after morning milking of Manchega ewes at 15, 30, 45, 60, 75, 90, 105, 120, and 135 days after parturition. The animals did not receive any kind of treatment or medicated feed throughout the experiment. The false-positive rates for BRT and Delvotest were 3.75 and 2.4%, respectively. When the logistic regression model was applied, the percentages of total solids for positive samples were significantly different from those for negative samples (16.90 versus 18.42% for BRT, 16.05 versus 18.45% for Delvotest), while the SCC logarithmic transformation was significantly higher for the positive samples than for the negative samples (5.38 versus 5.11 log units for BRT, 5.32 versus 5.11 log units for Delvotest). Moreover, Delvotest-positive samples exhibited thiocyanate concentrations higher than those of Delvotest-negative samples (8.18 mg/liter versus 6.85 mg/liter). Further analyses are needed to confirm the possible presence of antimicrobial residues in this particular type of milk sample. 相似文献
85.
This article sets forth the main findings of an empirical study carried out among the main actors in the sector of large-scale retailing in Europe, as regards their respective practical experience incrm (Customer Relationship Management) as well as the opportunities offered by the Internet technologies in this field. The evidence collected is checked against the problems expounded in the literature hinging on the following four main questions: —the existence of national orientations that are taking shape in terms of the uses ofcrm —the reality of the training processes of companies leading to e-crm as well as the acquisition of the technical and organisational maturity necessary to master these tools —the variety of those uses and the issue of multichannelling —the relative benefits of market relationship linked to the development ofcrm procedures Thus this study offers an overview of emerging trends incrm peculiar to this sector. 相似文献
86.
Lilienthal ND Alsafar H Conerty J Fernandez R Kong C Smith DK 《Analytical chemistry》2003,75(14):3322-3328
Electrodes modified with Nafion films containing 2,7-dimethyldiazapyrenium (DAP2+) were prepared and characterized with voltammetry by themselves and in the presence of organic substrates. The large, planar, electron-poor aromatic surface in DAP2+ facilitates pi-stacking interactions with other planar aromatic molecules, particularly those that are negatively charged or electron-rich. Previous studies showed that the reduction of DAP2+ decreases the strength of these interactions, making the binding redox-dependent, and resulting in negative shifts in the E(1/2) of DAP2+/+. This study shows that the redox-dependent binding ability of DAP2+ is retained in Nafion, but the selectivity is considerably different. Most significantly, the electron-rich, neutral aromatic compounds that produced small shifts in the E(1/2) of DAP2+/+ in solution cause much larger shifts, up to -110 mV, with the modified electrodes. With indole as a substrate, Nernstian behavior is observed (-60 mV shift per log[indole]) between 10 and 0.5 mM. 相似文献
87.
Detection of tyrosine phosphorylated peptides by precursor ion scanning quadrupole TOF mass spectrometry in positive ion mode 总被引:9,自引:0,他引:9
Phosphorylation is a common form of protein modification. To understand its biological role, the site of phosphorylation has to be determined. Generally, only limited amounts of phosphorylated proteins are present in a cell, thus demanding highly sensitive procedures for phosphorylation site determination. Here, a novel method is introduced which enables the localization of tyrosine phosphorylation in gel-separated proteins in the femtomol range. The method utilizes the immonium ion of phosphotyrosine at m/z 216.043 for positive ion mode precursor ion scanning combined with the recently introduced Q2-pulsing function on quadrupole TOF mass spectrometers. The high resolving power of the quadrupole TOF instrument enables the selective detection of phosphotyrosine immonium ions without interference from other peptide fragments of the same nominal mass. Performing precursor ion scans in the positive ion mode facilitates sequencing, because there is a no need for polarity switching or changing pH of the spraying solvent. Similar limits of detection were obtained in this approach when compared to triple-quadrupole mass spectrometers but with significantly better selectivity, owing to the high accuracy of the fragment ion selection. Synthetic phosphopeptides could be detected at 1 fmol/microL, and 100 fmol of a tyrosine phosphorylated protein in gel was sufficient for the detection of the phosphorylated peptide in the unseparated digestion mixture and for unambiguous phosphorylation site determination. The new method can be applied to unknown protein samples, because the identification and localization of the modification is performed on the same sample. 相似文献
88.
In this work, we present the principles and potential advantages of a methodology to assess Fourier components in terms of phase consistency. We define a new phase consistency criterion among sets of spatially translated images based upon a novel conception of the spatial shift property of the Fourier transform. The article shows how this criterion can be used in the alignment stage of the 3D reconstruction process with a two-fold objective: Assessment of the frequency components and robustness in the alignment. In that sense, the article shows and analyzes the results obtained from the application of the new index of quality in the context of projection image alignment. We have focussed our attention on the electron crystallography field, by applying such a phase consistency definition over image reflections. The results that have been obtained show that the new phase-consistency definition may complement the traditional SNR-based index of quality (commonly known as IQ) of reflections. As a consequence, the reliability of the alignment may be improved by discarding those reflections judged as non-reliable according to the phase-consistency criterion. 相似文献
89.
A coupled ab initio and thermodynamic study of the Al–H–Mg system has been carried out and a self-consistent thermodynamic database has been obtained. Magnesium alanate Mg(AlH4)2, a candidate material for hydrogen storage, has been included into the database. According to Density Functional first principles calculations, the alanate is an insulator and its thermodynamic properties have been obtained at room temperature. This compound has been found metastable at 298.15 K and 1 bar. The alanate has been found thermodynamically stable only at high pressure when the formation of the binary β-MgH2 phase is neglected. A reassessment of thermodynamic parameters of the liquid phase in the binary Mg–H system has also been carried out in order to be consistent with the Al–H system. The present results can reproduce reasonably well the available experimental data. 相似文献
90.
Fernandez Pantoja M. Rubio Bretones A. Garcia Ruiz F. Garcia S.G. Gomez Martin R. 《Antennas and Propagation Magazine, IEEE》2007,49(4):34-47
This paper presents a short tutorial and overview of optimization algorithms based on particle-swarm schemes, and their application to solving electromagnetic problems. As a practical example, a particle-swarm optimization (PSO) tool has been applied in conjunction with the Numerical Electromagnetics Code (NEC) to get the design curves of optimized log-periodic dipole arrays (LPDAs). The graphs show the performance of several radiation parameters (directive gain, front-to-back ratio, bandwidth, SWR, and half-power beamwidth in the E and H planes) as a function of typical design parameters of log-periodic dipole arrays (geometrical parameters and characteristics of the feed). Examples of optimized antennas are given, and their performance is compared to that of standard log-periodic dipole arrays. 相似文献