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71.
Gloria Lazzeri Carla L. Busceti Francesca Biagioni Cinzia Fabrizi Gabriele Morucci Filippo S. Giorgi Michela Ferrucci Paola Lenzi Stefano Puglisi-Allegra Francesco Fornai 《International journal of molecular sciences》2021,22(13)
Norepinephrine (NE) neurons and extracellular NE exert some protective effects against a variety of insults, including methamphetamine (Meth)-induced cell damage. The intimate mechanism of protection remains difficult to be analyzed in vivo. In fact, this may occur directly on target neurons or as the indirect consequence of NE-induced alterations in the activity of trans-synaptic loops. Therefore, to elude neuronal networks, which may contribute to these effects in vivo, the present study investigates whether NE still protects when directly applied to Meth-treated PC12 cells. Meth was selected based on its detrimental effects along various specific brain areas. The study shows that NE directly protects in vitro against Meth-induced cell damage. The present study indicates that such an effect fully depends on the activation of plasma membrane β2-adrenergic receptors (ARs). Evidence indicates that β2-ARs activation restores autophagy, which is impaired by Meth administration. This occurs via restoration of the autophagy flux and, as assessed by ultrastructural morphometry, by preventing the dissipation of microtubule-associated protein 1 light chain 3 (LC3) from autophagy vacuoles to the cytosol, which is produced instead during Meth toxicity. These findings may have an impact in a variety of degenerative conditions characterized by NE deficiency along with autophagy impairment. 相似文献
72.
Pasini F Verardo V Cerretani L Caboni MF D'Antuono LF 《Journal of the science of food and agriculture》2011,91(15):2858-2864
BACKGROUND: Salad crops of the Brassicaceae family, such as Diplotaxis tenuifolia and Eruca vesicaria, commonly referred to as ‘rocket salads’, have attracted considerable interest as culinary vegetables because of their strong flavour and their content of putative health‐promoting compounds. Among such compounds, glucosinolates and phenolics are well‐known phytochemicals with an important role also in determining the characteristic flavour of these species. In this study, to identify potentially high‐value rocket salads, 37 cultivated types were examined for sensory characters and their relations with glucosinolate and phenolic contents, which ranged from 0.76 to 3.03 g kg?1 dry weight (DW) and from 4.68 to 31.39 g kg?1 DW, respectively. RESULTS: The perception of bitter taste was significantly affected by specific glucosinolates, namely progoitrin/epiprogoitrin and dimeric glucosativin. Aroma intensity was negatively related to glucoalyssin content, whereas pungency was significantly related to total glucosinolate content. Kaempferol‐3‐(2‐sinapoyl‐glucoside)‐4′‐glucoside was positively and significantly related to all flavour trait perceptions. Aroma intensity, pungency, crunchiness and juiciness were positively related to typical rocket salad flavour perception through a prominent direct effect. CONCLUSION: Aroma intensity, pungency, crunchiness and juiciness were strong determinants of overall rocket salad flavour perception. Visual traits also characterised sensory components. Bitterness, usually considered a negative flavour trait, was moderately perceived in the examined material, without negatively affecting typical flavour perception. In the range of the examined material, glucosinolate content did not contrast with typical flavour, demonstrating that good taste and putative health‐promoting properties may coexist. Copyright © 2011 Society of Chemical Industry 相似文献
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75.
Cappiello A Famiglini G Fiorucci C Mangani F Palma P Siviero A 《Analytical chemistry》2003,75(5):1173-1179
A new, simple device generates accurate nano- and microflow rate gradients from any conventional HPLC system. The core of the new device is represented by an electric-actuated, computer-controlled, multiposition HPLC valve. The valve hosts six reservoirs for as many different mobile-phase compositions of increasing strength. A low flow rate stream pushes the weakest solvent through the column as long as required and at the desired flow rate, until the chromatographic run is started. From this time on, the electric actuation allows one to select which reservoir will be on-line with the column and for how long, thus generating a specific solvent gradient, through a sequence of controlled segments of precise mobile-phase composition. This permits one not only to exactly reproduce the programmed slope but also to achieve different gradient shapes (i.e., linear, convex, concave) for different separation needs. The new device has proven to be reliable and reproducible even at the lowest flow rate tested (250 nL x min(-1)) and in different chromatographic conditions. 相似文献
76.
Limnological features of Funil Reservoir (R.J., Brazil) and indicator properties of rotifers and cladocerans of the zooplankton community 总被引:3,自引:0,他引:3
Christina W. C. Branco Maria-Isabel A. Rocha Gláucia F. S. Pinto Gisele A. Gômara Rodrigo De Filippo 《Lakes & Reservoirs: Research and Management》2002,7(2):87-92
Funil Reservoir receives inflow from a highly industrialized region and acts as a natural sink to pollutants. Among the consequences of the uncontrolled nutrient loading is the constant presence and periodic heavy blooms of Microcystis aeruginosa . This study verified limnological features and zooplankton assemblage of the Funil Reservoir focusing on the environmental-indicator properties of rotifers and cladocerans. The summer bloom of M. aeruginosa caused reductions in water transparency, nitrate and orthophosphate concentrations and raises in chlorophyll a , pH, dissolved oxygen and chemical oxygen demand values. All zooplankton species presented spatial and temporal variations with the exception of the copepods, which were present in all samples. According to canonical correspondence analysis, ammonium was the variable most related to zooplankton variability and different Rotifera and Cladocera assemblages indicated distinct environment conditions. Rotifer taxa associated with increases of water temperature and chlorophyll a concentration were found with high densities during M. aeruginosa blooms. Species of rotifer and cladocerans are suggested as indicators that can be used to identify different physical and chemical gradients or eutrophic increases in Funil Reservoir. 相似文献
77.
Giorgia Sparapassi Stefano M.Cavaletto Jonathan Tollerud Angela Montanaro Filippo Glerean Alexandre Marciniak Fancesca Giusti Shaul Mukamel Daniele Fausti 《光:科学与应用(英文版)》2022,(3):357-364
We present a novel approach to transient Raman spectroscopy, which combines stochastic probe pulses and a covariance-based detection to measure stimulated Raman... 相似文献
78.
Optimal ensemble construction via meta-evolutionary ensembles 总被引:1,自引:0,他引:1
In this paper, we propose a meta-evolutionary approach to improve on the performance of individual classifiers. In the proposed system, individual classifiers evolve, competing to correctly classify test points, and are given extra rewards for getting difficult points right. Ensembles consisting of multiple classifiers also compete for member classifiers, and are rewarded based on their predictive performance. In this way we aim to build small-sized optimal ensembles rather than form large-sized ensembles of individually-optimized classifiers. Experimental results on 15 data sets suggest that our algorithms can generate ensembles that are more effective than single classifiers and traditional ensemble methods. 相似文献
79.
80.
Filippo Lanubile 《Empirical Software Engineering》1997,2(2):97-108
Technology evaluation is part of the decision-making process of any software organization. Unlike conventional wisdom, empirical
evaluation strives to avoid biased conclusions by relying on observation and looking for pitfalls in the evaluation process.
In this paper, we provide a summary of the maintenance studies presented in the session ‘Study and assessment of (new) technologies’
of the International Workshop on Empirical Studies of Software Maintenance (WESS '96), and also report on the working group
discussion which focused on common problems and open issues in the field of technology evaluation. These empirical studies
are then classified according to a multi-dimensional framework to synthesize the state of the research in technology evaluation
and ultimately discover interesting patterns.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献