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排序方式: 共有650条查询结果,搜索用时 15 毫秒
81.
A preparation of the bacteriocin lacticin 3147 (prepared from a demineralized whey protein fermentation liquor) was combined as a powder with a bismuth-based intramammary teat seal and evaluated for its potential as an antimicrobial in non-lactating cows. The lacticin/teat seal formulation enabled significant bacteriocin release from the seal without the requirement for a surfactant. Studies in vivo in lactating cows demonstrated that this formulation was effective in reducing bacterial recoveries (approximately 20-fold) from teats deliberately inoculated with Staphylococcus aureus after infusion. Moreover, this formulation also significantly reduced the numbers of Staph. aureus recovered from teats that were exposed to the challenge bacterium before the infusion of the teat seal preparation. The powdered preparation of lacticin 3147 did, however, cause some teat irritation as evidenced by associated rises in somatic cell count (SCC). However, this effect was short-lived and when the mean SCC readings pre-infusion and the final two readings post-infusion were compared, there was no significant difference in the immunological acceptance between treatments. 相似文献
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X Li NK Lee YW Ye PG Waber C Schweitzer QC Cheng PD Nisen 《Canadian Metallurgical Quarterly》1994,86(20):1524-1529
BACKGROUND: Little is known about the molecular genetic events that contribute to the pathogenesis of squamous cell carcinoma of the upper aerodigestive tract. Previous molecular genetic studies have been limited to the identification of mutations of the p53 (also known as TP53) tumor suppressor gene, activation of a limited set of oncogenes, allelic loss at 3p and other locations, and occasional association with human papillomavirus infection. PURPOSE: Our purpose was to screen tumor tissue and blood from patients with squamous cell carcinoma of the upper aerodigestive tract for loss of heterozygosity at polymorphic loci corresponding to each of the autosomal chromosomes and to identify the locations of additional putative tumor suppressor genes, other than RB (also known as RB1) and p53, that may contribute to the pathogenesis of this disease. METHODS: Tumor tissue and blood were obtained from 68 consecutive patients with squamous cell carcinoma of the upper aerodigestive tract. In all cases, tumor tissue was obtained from the center of the surgical specimen. The relative absence of non-neoplastic tissue was confirmed by frozen-section histologic examination of immediately adjacent tissue. Initially, 30 paired tissue and blood samples were tested for loss of heterozygosity by polymerase chain reaction (PCR) to amplify 43 different highly polymorphic sequences containing small oligonucleotide repeats. After PCR amplification, with unique oligonucleotides flanking the repeat, visualization and sizing of the alleles on DNA sequencing gels were performed. Specific loss of heterozygosity was distinguished from random genetic loss due to generalized chromosomal instability if it occurred in more than 20% of specimens tested for a particular marker. RESULTS: Significant loss of heterozygosity (> 20%) occurred at alleles at chromosome bands 3p21 (32%), 3p25-26 (56%), 8pter-21.1 (31%), 13q14 (27%), and 17p12 (45%). Loss of heterozygosity at more than two loci was significant with a poor prognosis (P = .039). CONCLUSIONS: These findings demonstrate that squamous cell carcinoma of the upper aerodigestive tract exhibits genetic alterations at multiple loci and that allelic loss at more than two locations is indicative of a poor prognosis (the likelihood of the patient dying of disease). IMPLICATIONS: While tumor suppressor genes at 3p (VHL), 13q (RB), and 17p (p53) have been identified, altered genes at other loci on 3p and on 8p have not yet been characterized. Furthermore, the genotype at these loci for squamous cell carcinoma of the upper aerodigestive tract has prognostic importance and may identify the patients who should receive the most aggressive treatment. 相似文献
85.
J. Moldenhauer H. Wachtel D. Schweitzer P. Bele H. Brunner H. J. Keller B. Gompf W. Eisenmenger 《Synthetic Metals》1995,70(1-3)
α-(BEDT-TTF)2I3 is a quasi-two-dimensiona1 organic metal with a metal-insulator phase transition at 135 K. Thermal treatment at about 80°C leads to the metallic system α1-(BEDT-TTF)2I3 which becomes superconducting below 8 K. Thin films of the α-phase (between 500 and 3000 A thick) have been evaporated in high vacuum onto several substrates and characterised by means of X-ray diffraction, scanning electron microscopy (SEM), atomic force microscopy (AFM) and a low field microwave absorption technique. Depending on the temperature of the substrate and the evaporation rate, the films exhibit different degrees of microcristallinity, which under certain conditions can be strongly reduced and a completely covering film can be obtained. X-ray diffraction spectra reveal a high orientation with the c-axis perpendicular to the substrate and as well the successful conversion into the α1- phase by tempering. SEM and AFM investigations prove that the conversion takes place without reducing the mechanical quality of the films. Low-field microwave-absorption experiments show that the α1-films become superconducting with an onset at 9 K. 相似文献
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R Schweitzer 《Canadian Metallurgical Quarterly》1993,22(7):1207-1208
Thinking of treating your family?--think again! There are risks involved as objectivity is in danger of being clouded by emotions. Guidelines for the 'family' doctor are drawn from the author's experience. 相似文献
88.
Measurements of the de Haas-van Alphen (dHvA) effect, the complex susceptibility and the specific heat, C, of κ-(ET)2I3 are reported. The strong harmonic content of the dHvA signal and the upper critical field, Bc2, following the angular dependence of thin films as described by the Tinkham formula reveal the almost perfect two-dimensionality of this compound. The specific heat shows a jump at Tc consistent with BCS. Some deviations in the temperature dependence of C might be explained by strong coupling. 相似文献
89.
Bekaert B Cooper ML Green FR McNulty H Pentieva K Scott JM Molloy AM Rayman MP 《Molecular nutrition & food research》2008,52(11):1324-1333
The level of plasma total homocysteine (tHcy), long known to be B vitamin dependent, has recently been shown to be inversely associated with plasma selenium (Se) concentration in human subjects. We therefore, chose to investigate the interaction between Se, tHcy and B vitamins in a double-blind, placebo-controlled trial where 501 healthy UK elderly volunteers were randomly allocated to receive 100, 200, or 300 microg Se/day as high-Se-yeast, or placebo-yeast for 6 months. Plasma Se, tHcy, folate, vitamin B-12, pyridoxal-5'-phosphate (PLP) and its catabolite, 4-pyridoxic acid, were measured in all participants at baseline and in samples from the placebo, 100 and 300 microg Se/day groups, at follow-up. At baseline, Se was inversely correlated with tHcy but only in males (p < 0.001). Before supplementation, tHcy concentration was significantly lower in the highest compared to the lowest Se tertile in males (p < 0.05), and in females when folate concentrations were also in the top tertile (p < 0.05). The effect of folate, PLP and vitamin B-12 concentrations on plasma tHcy correlated with Se concentration at baseline. After 6 months of Se supplementation, only Se concentration had changed significantly. Supplementation with Se does not affect tHcy concentration in the UK elderly population. 相似文献
90.