Modulation of Cellular Fate of Vinyl Triarylamines through Structural Fine Tuning: To Stay or Not To Stay in the Mitochondria?

Mitochondria are involved in many cellular pathways and dysfunctional mitochondria are linked to various diseases. Hence efforts have been made to design mitochondria-targeted fluorophores for monitoring the mitochondrial status. However, the factors that govern the mitochondria-targeted potential of dyes are not well-understood. In this context, we synthesized analogues of the TP-2Bzim probe belonging to the vinyltriphenylamine (TPA) class and already described for its capacity to bind nuclear DNA in fixed cells and mitochondria in live cells. These analogues ( TP-1Bzim, TPⁿ-2Bzim, TP¹⁺-2Bzim, TN-2Bzim ) differ in the cationic charge, the number of vinylbenzimidazolium branches and the nature of the triaryl core. Using microscopy, we demonstrated that the cationic derivatives accumulate in mitochondria but do not reach mtDNA. Under depolarisation of the mitochondrial membrane, TP-2Bzim and TP¹⁺-2Bzim translocate to the nucleus in direct correlation with their strong DNA affinity. This reversible phenomenon emphasizes that these probes can be used to monitor ΔΨ_m variations.     

Pictures worth a thousand tiles,a geometrical programming language for self-assembly

We present a novel way to design self-assembling systems using a notion of signal (or ray) akin to what is used in analyzing the behaviour of cellular automata. This allows purely geometrical constructions, with a smaller specification and easier analysis. We show how to design a system of signals for a given set of shapes, and how to transform these signals into a set of tiles which self-assemble into the desired shapes.     

Synthesis of Spiro[piperidine‐3,3′‐oxindoles] via Gold(I)‐Catalyzed Dearomatization of N‐Propargyl‐ and N‐Homoallenyl‐2‐bromotryptamines

N‐Propargyl‐ and N‐homoallenyl‐2‐bromo‐β‐tryptamines undergo gold(I)‐catalyzed dearomatizing cyclizations to afford 2‐bromospiroindolenines that are in situ hydrolyzed to furnish spirooxindoles in a one‐pot process. Tryptophane derivatives (R²=CO₂Et) led upon cyclization to chiral spirooxindoles in excellent diastereoselectivities.

     

Vertically aligned carbon nanotube‐based composite: Elaboration and monitoring of the nanotubes alignment

We present the different elaboration steps of a composite formed of carbon nanotubes (CNT) carpet embedded in an epoxy polymer. Detailed characterization at each step of the elaboration process is performed. The good alignment of CNT in as‐grown carpets is kept all along the elaboration process of the composite, as it is measured at both macro and microscopic scales by X‐ray scattering. We also ensured by X‐ray fluorescence measurements that the iron‐based catalyst particles used for the synthesis were removed from the carpet after a high temperature post‐annealing treatment. These measurements give valuable information for further applications involving unidirectional nanotube composites and membranes, where CNT alignment is a key parameter. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 39730.     

A gradient-based path optimization method for motion planning

Most algorithms in probabilistic sampling-based path planning compute collision-free paths made of straight line segments lying in the configuration space. Due to the randomness of sampling, the paths make detours that need to be optimized. The contribution of this paper is to propose a basic gradient-based algorithm that transforms a polygonal collision-free path into a shorter one. While requiring only collision checking, and not any time-consuming obstacle distance computation nor geometry simplification, we constrain only part of the configuration variables that may cause a collision, and not entire configurations. Thus, parasite motions that are not useful for the problem resolution are reduced without any assumption. Experimental results include navigation and manipulation tasks, eg a manipulator arm-filling boxes and a PR2 robot working in a kitchen environment. Comparisons with a random shortcut optimizer and a partial shortcut have also been studied.     

Reachability and confluence are undecidable for flat term rewriting systems

Ground reachability, ground joinability and confluence are shown undecidable for flat term rewriting systems, i.e., systems in which all left and right members of rule have depth at most one.     

HDM: A Client/Server/Engine Architecture for Real-Time Web Usage Mining

The behavior of the users of a website may change so quickly that it becomes a real challenge to attempt to make predictions according to the frequent patterns coming from the analysis of an access log file. In order to reduce the obsolescence of behavioral patterns as much as possible, the ideal method would provide frequent patterns in real time, making the result immediately available. In this paper, we propose a method for finding frequent behavioral patterns in real time, whatever the number of connected users. Considering how fast frequent behavior patterns may have changed since the time the access log file was analyzed, this result thus provides completely appropriate navigation schemata for predicting user behavior. Based on a distributed heuristic, our method also tackles and provides answers to several problems within the framework of data mining: the discovery of interesting zones (a large number of frequent patterns concentrated over a period of time, or super-frequent patterns), discovering very long sequential patterns and interactive data mining (on-the-fly modification of the minimum support).     

Stabilization of slugging in oil production facilities with or without upstream pressure sensors

This paper presents methods for suppressing the slugging phenomenon occurring in multiphase flow. The considered systems include industrial oil production facilities such as gas-lifted wells and flowline risers with low-points. Given the difficulty to maintain sensors in deep locations, a particular emphasis is put on observer-based control design. It appears that, without any upstream pressure sensor, such a strategy can stabilize the flow. Besides, given a measurement or estimate of the upstream pressure, we propose a control strategy alternative to the classical techniques. The efficiency of these methods is assessed through experiments on a mid-scaled multiphase flow loop.     

On the composition of time Petri nets

Complex systems are often designed and built from smaller pieces, called components. Components are open sub-systems meant to be combined (or composed) to form other components or closed systems. It is well known that Petri nets allow such a component based modeling, relying on parallel composition and transition synchronization. However, synchronizing transitions that carry temporal constraints does not yield a compositional method for assembling components, a highly desirable property. The paper addresses this particular problem: how to build complex systems in a compositional manner from components specified by Time Petri nets (TPN). A first solution is proposed, adequate for a particular subclass of Time Petri nets but significantly increasing the complexity of components. Then an improved solution is developed, relying on an extension of Time Petri nets with two relations added on transitions. This latter solution requires a much simpler transformation of nets, does not significantly increase their complexity, and is applicable to a larger class of TPN.