Dissimilarity-based classification of chromatographic profiles

This paper proposes a non-parametric method for the classification of thin-layer chromatographic (TLC) images from patterns represented in a dissimilarity space. Each pattern corresponds to a mixture of Gaussian approximation of the intensity profile. The methodology comprises various phases, including image processing and analysis steps to extract the chromatographic profiles and a classification phase to discriminate among two groups, one corresponding to normal cases and the other to three pathological classes. We present an extensive study of several dissimilarity-based approaches analysing the influence of the dissimilarity measure and the prototype selection method on the classification performance. The main conclusions of this paper are that, Match and Profile-difference dissimilarity measures present better results, and a new prototype selection methodology achieves a performance similar or even better than conventional methods. Furthermore, we also concluded that simplest classifiers, such as k-NN and linear discriminant classifiers (LDCs), present good performance being the overall classification error less than 10% for the four-class problem.     

Quantitative comparison of thermal and solutal transport in a T-mixer by FLIM and CFD

The development and adoption of lab-on-a-chip and micro-TAS (total analysis system) techniques requires not only the solving of design and manufacturing issues, but also the introduction of reliable and quantitative methods of analysis. In this work, two complementary tools are applied to the study of thermal and solutal transport in liquids. The experimental determination of the concentration of water in a water–methanol mixture and of the temperature of water in a microfluidic T-mixer are achieved by means of fluorescence lifetime imaging microscopy (FLIM). The results are compared to those of finite volume simulations based on tabulated properties and well-established correlations for the fluid properties. The good correlation between experimental and modelled results demonstrate without ambiguity that (1) the T-mixer is an adiabatic system within the conditions, fluids and flow rates used in this study, (2) buoyancy effects influence the mixing of liquids of different densities at moderate flow rates (Reynolds number Re ≪ 10⁻²), and (3) the combination of FLIM and computational fluid dynamics has the potential to be used to measure the thermal and solutal diffusion coefficients of fluids for a range of temperatures and concentrations in one single experiment. As such, it represents a first step towards the full-field monitoring of both the extent and the kinetics of a chemical reaction.

---

Food vacuole proteome of the malarial parasite Plasmodium falciparum

The Plasmodium falciparum food vacuole (FV) is a lysosome-like organelle where erythrocyte hemoglobin digestion occurs. It is a favorite target in the development of antimalarials. We have used a tandem mass spectrometry approach to investigate the proteome of an FV-enriched fraction and identified 116 proteins. The electron microscopy analysis and the Western blot data showed that the major component of the fraction was the FV and, as expected, the majority of previously known FV markers were recovered. Of particular interest, several proteins involved in vesicle-mediated trafficking were identified, which are likely to play a key role in FV biogenesis and/or FV protein trafficking. Recovery of parasite surface proteins lends support to the cytostomal pathway of hemoglobin ingestion as a FV trafficking route. We have identified 32 proteins described as hypothetical in the databases. This insight into FV protein content provides new clues towards understanding the biological function of this organelle in P. falciparum.     

Occupational health problems and injuries among Quebec's physical educators

The purpose of this study was to describe the traumatology of teaching physical education (PE) and to document the causes and mechanisms of injuries in this occupation. Our sample was composed of 314 physical educators (PEs) teaching at three school levels (primary school, secondary school and college) who responded to a questionnaire survey. The results showed a 0.55 rate of recent lesion per teacher per year (0.65 for women and 0.51 for men). A total of 37.6% of subjects were affected by a lesion during the last year at work. Subjects with a higher weekly frequency of leisure physical activities or endurance activities (five times and more) were less affected by chronic lesions as well as those who frequently practiced aerobic physical activities. Prevalence of chronic injuries was higher in older teachers. Our results confirm that the rate of lesions in this group of workers is high, and that school level and respondent age may influence the rate of lesion and the characteristics of risk factors. In particular, the effect of ageing and teaching specialization on the incidence of occupational problems in PEs warrants further research. Given the relatively high rate of injuries among PE teachers, we suggest that it is necessary to further explore preventive strategies to reduce occupational risk in these workers. In particular, prophylactic physical activity outside of work may represent such a strategy since PEs more active outside their work have lower chronic injury prevalence. PEs gave long periods of standing the highest rating for movements or positions most likely to cause them occupational injuries. It can thus be suggested to find ways to reduce standing position sustained for long periods.     

An Approach for Recovering Distributed System Architectures

Reasoning about software systems at the architectural level is key to effective software development, management, evolution and reuse. All too often, though, the lack of appropriate documentation leads to a situation where architectural design information has to be recovered directly from implemented software artifacts. This is a very demanding process, particularly when involving recovery of runtime abstractions (clients, servers, interaction protocols, etc.) that are typical to the design of distributed software systems. This paper presents an exploratory reverse engineering approach, called X-ray, to aid programmers in recovering architectural runtime information from a distributed system's existing software artifacts. X-ray comprises three domain-based static analysis techniques, namely component module classification, syntactic pattern matching, and structural reachability analysis. These complementary techniques can facilitate the task of identifying a distributed system's implemented executable components and their potential runtime interconnections. The component module classification technique automatically distinguishes source code modules according to the executables components they implement. The syntactic pattern matching technique in turn helps to recognise specific code fragments that may implement typical component interaction features. Finally, the structural reachability analysis technique aids in the association of those features to the code specific for each executable component. The paper describes and illustrates the main concepts underlying each technique, reports on their implementation as a suit of new and off-the-shelf tools, and, to give evidence of the utility of the approach, provides a detailed account of a successful application of the three techniques to help recover a static approximation of the runtime architecture for Field, a publicly-available distributed programming environment.     

Quantum chemical studies on protonation of some substituted thiazole derivatives

The acidity constants, pK(a) values for protonation of some substituted thiazole derivatives were calculated by using AM1 and PM3 basis sets of semi-empirical methods and B3LYP/6-31G(d) basis sets of density functional theory (DFT) calculated physical and thermodynamic parameters. Correlation search among the experimental and calculated acidity constants, pK(a) values, revealed that the best correlation exist between the experimental and ab initio calculated pK(a) values with a regression of R(2)=0.98.     

Optimization of media processing workflows with adaptive operator behaviors

In this paper, we present the ARIA media processing workflow architecture that processes, filters, and fuses sensory inputs and actuates responses in real-time. The components of the architecture are programmable and adaptable; i.e. the delay, size, and quality/precision characteristics of the individual operators can be controlled via a number of parameters. Each data object processed by qStream components is subject to transformations based on the parameter values. For instance, the quality of an output data object and the corresponding processing delay and resource usage depend on the values assigned to parameters of the operators in the object flow path. In Candan, Peng, Ryu, Chatha, Mayer (Efficient stream routing in quality- and resource-adaptive flow architectures. In: Workshop on multimedia information systems, 2004), we introduced a class of flow optimization problems that promote creation and delivery of small delay or small resource-usage objects to the actuators in single-sensor, single-actuator workflows. In this paper, we extend our attention to multi-sensor media processing workflow scenarios. The algorithms we present take into account the implicit dependencies between various system parameters, such as resource consumption and object sizes. We experimentally show the effectiveness and efficiency of the algorithms.

How do designers represent to themselves the users' needs?

This paper reports on an ergonomic study carried out during the design of a cutting machine-tool for the composite material in carbody parts casting. During this design process, the users’ needs were inferred by the designers on the basis of their own mental representations of the use of the new device. These representations of the users’ needs, correct or false, play a decisive role in the choice of a solution. The aim of the study is to identify their particularity. Analyzing the design meetings, we have highlighted that users are considered either as subsystems or basic design principles or elements of an imagined scenario. We have shown that these representations are linked to the types of meetings held during the design process. Accordingly, a diversification of the types of meetings should be promoted by the project leader, so that designers extend their points of view of the operators.     

Combining ergonomics, culture and scenario for the design of a cooperation platform

Analyzing the way computer technologies are used is crucial for their development. Such analyses make it possible to evaluate these technologies and enhance their evolution. The present article presents some ideas drawn from the development of a cooperation platform for elementary school children (10–11 years old). On the basis of an obvious ergonomic requirement, we worked on two other dimensions: cultural aspects and the teaching scenario. The goal was to set up observation situations and analyze the conversations produced during those situations, in order to understand what using the platform meant to both the pupils and their teachers.