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81.
Fujio Takahashi Ernst -H. Reimerdes Henning Klostermeyer 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1977,164(3):163-166
Summary In casein-containing agarose gels, pepsin and chymosin form radial diffusion zones; the diameters of these zones show rectilinear correlations with the logarithm of the enzyme concentration at constant time. The sensitivity for both enzymes is below 1 g. Addition of the inhibitor pepstatin A to these enzymes causes a reduction of the diameters of the diffusion zones, with large differences for both the enzymes. With this procedure, the pepsin/chymosin ratio in rennet preparations was assayed with an accuracy of ±5%. Identification of the inhibitors allows the determination of amounts in the namomole range. This method is a simple technique for the evaluation of proteinases and their inhibitors in screening systems.
Geldiffusion — eine einfache und empfindliche Technik für den Nachweis von Proteinaseinhibitoren und die Anwendung für die Bestimmung von Proteinasen in Gemischen
Zusammenfassung In caseinhaltigen Agarosegelen erzeugen sowohl Pepsin wie Chymosin radiale Diffusionszonen, deren Durchmesser bei gegebener Zeit dem Logarithmus der Enzymmenge proportional ist. Die Bestimmungsgrenze liegt für beide Enzyme unter 1 g. Bei Zugabe des Inhibitors Pepstatin A zu den Proteinasepräparaten werden die Diffusionszonen beider Proteinasen verkleinert, aber in unterschiedlichem Ausmaße. Auf dieser Basis kann in technischen Labpräparaten das Mengenverhältnis Pepsin/Chymosin mit einer Genauigkeit von ±5% bestimmt werden. Bei Umkehr der Reaktion zum Nachweis des Inhibitors sind Mengen im Bereich von 10–9 Mol sicher erfaßbar. Die Methodik ist verallgemeinerungsfähig als Screening-Test für Proteinaseinhibitoren.相似文献
82.
Allinson M Kageyama S Nakajima D Kamata R Shiraishi F Goto S Salzman SA Allinson G 《Water science and technology》2012,66(4):768-774
In 2007, samples of treated effluent were collected at point of discharge to the environment from 39 wastewater treatment plants (WWTPs) located across Victoria, Australia grouped by treatment type. Sample genotoxicity was assessed with a high-throughput luminescent umu test method using Salmonella typhimurium TL210 strain, with and without addition of a commercially available metabolic activation system. Samples were also screened using a gas chromatographic-mass spectrometric mass-structure database recognition method. A genotoxic response was observed in half of the samples tested without metabolic activation system (相似文献
83.
Tojo H Ejiri A Hiratsuka J Yamaguchi T Takase Y Itami K Hatae T 《The Review of scientific instruments》2012,83(2):023507
This paper presents an experimental demonstration to determine electron temperature (T(e)) with unknown spectral sensitivity (transmissivity) in a Thomson scattering system. In this method, a double-pass scattering configuration is used and the scattered lights from each pass (with different scattering angles) are measured separately. T(e) can be determined from the ratio of the signal intensities without knowing a real chromatic dependence in the sensitivity. Note that the wavelength range for each spectral channel must be known. This method was applied to the TST-2 Thomson scattering system. As a result, T(e) measured from the ratio (T(e,r)) and T(e) measured from a standard method (T(e,s)) showed a good agreement with <∣T(e,r) - T(e,s)∣∕T(e,s)> = 7.3%. 相似文献
84.
Koji Ishida Ken Hirota Osamu Yamaguchi Hideki Kume Suguru Inamura Hiroki Miyamoto 《Journal of the American Ceramic Society》1994,77(5):1391-1395
In the system ZrO2 –Al2 O3 , a new method for preparing ZrO2 solid solutions from ZrCl4 and AlCl3 using hydrazine monohydrate is investigated. c -ZrO2 solid solutions containing up to ∼40 mol% Al2 O3 crystallize at low temperatures from amorphous materials. The formation mechanism is discussed from IR spectral data. The values of the lattice parameter α increase linearly from 0.5072 to 0.5105 nm with increasing Al2 O3 content. At higher temperatures, transformation of the solid solutions proceeds as follows: c ( SS ) → t ( ss ) → t ( ss ) +α-Al2 O3 → m +α-Al2 O3 . m -ZrO2 –α-Al2 O3 composite ceramics are fabricated by hot isostatic pressing for 2 h at 1250°C and 196 MPa. Microstructures and mechanical properties are examined, in connection with increasing Al2 O3 content. 相似文献
85.
M Miyata T Yamamoyo M Yamaguchi K Nakao T Yoshida 《Canadian Metallurgical Quarterly》1976,152(4):540-543
Serial measurements of plasma "true glucagon" (PG) and of glucagon-like immunoreactive materials (GLI) were carried out during and after total resection of the pancreas in a 62-year-old man with calcified pancreatitis. The postoperative course of this patient was uneventful and diabetes was well controlled. PG disappeared from the blood within 30 min after resection of the pancreas. In spite of the evidence that no pancreatic tissue was present in the abdomen, PG was detected again in the blood from 18 hr after total pancreatectomy until the ninth postoperative day. However, plasma PG did not rise following infusion of arginine during the fourteenth postoperative week. After an initial decrease, plasma GLI rose abruptly on the second postoperative day and remained elevated thereafter. The fluctuations of plasma PG and GLI were not parallel. 相似文献
86.
Yasuyuki Kaneno Tetsuo Yamaguchi Takayuki Takasugi 《Journal of Materials Science》2006,41(20):6871-6880
The texture evolution during the hot-rolling and the recrystallization of B2-type Fe–48Al, Ni–50Al and Co–50Ti (expressed
by at.%) intermetallic compounds were investigated. By hot-rolling at 973 K, Fe–48Al showed a microstructure with coarse grains
elongated along rolling direction, while Ni–50Al and Co–50Ti showed a deformed microstructure featured by the heavily distorted
(elongated) grains and/or the deformation bands. The hot-rolling texture of Fe–48Al was composed of {111}<uvw>, while those
of Ni–50Al and Co–50Ti were composed of {111}<110> and {111}<112>, respectively. After annealing, the recrystallized grains
were preferentially nucleated at the grain boundaries for Fe–48Al, and in the heavily distorted regions or the deformation
bands for Ni–50Al and Co–50Ti. The orientations of the recrystallized grains were similar with those of the deformed matrix,
especially for Ni–50Al and Co–50Ti. The recrystallization textures were generally more dispersive than the hot-rolling texture.
Based on these results, the texture evolution during the hot rolling and the recrystallization of the B2-type intermetallic
compounds were discussed. 相似文献
87.
K. Daoudi T. Tsuchiya I. Yamaguchi T. Manabe S. Mizuta T. Kumagai 《Journal of Electroceramics》2006,16(4):527-532
Epitaxial La0.7Ca0.3MnO3 (LCMO) thin films were successfully prepared by the metal-organic deposition process on various (001) single-crystal substrates:
MgO, LaAlO3 (LAO), SrTiO3 (STO), and (LaAlO3)0.3-(SrAlTaO6)0.7 (LSAT). The crystallinity and the epitaxial growth of the LCMO films were characterized by X-ray diffraction (θ − 2θ scans
and pole-figure analysis). The temperature dependence of the resistance of the LCMO/LSAT, LCMO/STO and LCMO/LAO films exhibit
typical characteristics with a transition from the paramagnetic-insulator state to the ferromagnetic-metallic state at a temperature
peak (T
p
) ranging from 258 to 270 K. However, the LCMO/MgO films exhibited a semiconducting behavior without any transition. Based
on the R(T) measurement, we calculated the temperature coefficient of resistance (TCR) for a bolometric application and we obtained
22%/K, 10.2%/K and 27.5%/K for the film grown on the LSAT, STO and LAO substrates, respectively. This difference in the TCR
properties is related to the strain induced by the lattice mismatch between LCMO and the different substrates. 相似文献
88.
Saito W. Domon T. Omura I. Kuraguchi M. Takada Y. Tsuda K. Yamaguchi M. 《Electron Device Letters, IEEE》2006,27(5):326-328
A 13.56-MHz class-E amplifier with a high-voltage GaN HEMT as the main switching device is demonstrated to show the possibility of using GaN HEMTs in high-frequency switching power applications such as RF power-supply applications. The 380-V/1.9-A GaN power HEMT was designed and fabricated for high-voltage power-electronics applications. The demonstrated circuit achieved the output power of 13.4 W and the power efficiency of 91% under a drain-peak voltage as high as 330 V. This result shows that high-voltage GaN devices are suitable for high-frequency switching applications under high dc input voltages of over 100 V. 相似文献
89.
Hori T. Otani A. Kaiho K. Yamaguchi I. Morita M. Yanabu S. 《Applied Superconductivity, IEEE Transactions on》2006,16(4):1999-2004
Using a high-temperature superconductor, we constructed and tested a model superconducting fault current limiter (SFCL). The superconductor and the vacuum interrupter as the commutation switch were connected in parallel using a bypass coil. When the fault current flows in this equipment, the superconductor is quenched and the current is transferred to the parallel coil because of the voltage drop in the superconductor. This large current in the parallel coil actuates the magnetic repulsion mechanism of the vacuum interrupter. Due to the opening of the vacuum interrupter, the current in the superconductor is broken. By using this equipment, the current flow time in the superconductor can be easily minimized. On the other hand, the fault current is also easily limited by large reactance of the parallel coil 相似文献
90.
Toru Yamaguchi Makoto Sato Tomohiro Takagi 《Journal of Intelligent and Robotic Systems》1998,23(2-4):183-199
This paper proposes a multi-agent system that carries out cooperative work. To achieve this, we use Fuzzy Associative Memory Organizing Unit Systems (FAMOUS) and Conceptual Fuzzy Sets (CFS). By using these proposed methods, each agent robot can decide its own behaviour to suit the situation in its environment. We apply this system to an Intelligent Transportation System (ITS) and do simulations. 相似文献