Role of Intracellular Amyloid β as Pathway Modulator,Biomarker, and Therapy Target

The β- and γ-secretase-driven cleavage of the amyloid precursor protein (APP) gives rise to the amyloid β peptide, which is believed to be the main driver of neurodegeneration in Alzheimer’s disease (AD). As it is prominently detectable in extracellular plaques in post-mortem AD brain samples, research in recent decades focused on the pathological role of extracellular amyloid β aggregation, widely neglecting the potential meaning of very early generation of amyloid β inside the cell. In the last few years, the importance of intracellular amyloid β (iAβ) as a strong player in neurodegeneration has been indicated by a rising number of studies. In this review, iAβ is highlighted as a crucial APP cleavage fragment, able to manipulate intracellular pathways and foster neurodegeneration. We demonstrate its relevance as a pathological marker and shed light on initial studies aiming to modulate iAβ through pharmacological treatment, which has been shown to have beneficial effects on cognitive properties in animal models. Finally, we display the relevance of viral infections on iAβ generation and point out future directions urgently needed to manifest the potential relevance of iAβ in Alzheimer’s disease.     

GEOCHEMICAL EVALUATION OF CRUDE OILS FROM THE CARACARA AND TIPLE AREAS,EASTERN LLANOS BASIN,COLOMBIA: PALAEO BIODEGRADATION AND OIL MIXING

This study presents an organic geochemical characterization of heavy and liquid oils from Cretaceous and Cenozoic reservoir rocks in the Tiple and Caracara blocks in the eastern Llanos Basin, Colombia. Samples of heavy oil were recovered from the Upper Eocene Mirador Formation and the C7 interval of the Oligocene – Miocene Carbonera Formation; the liquid oils came from these intervals and from the Cretaceous Guadalupe, Une and Gachetá Formations. The heavy oil and most of the liquid oils probably originated from multiple source rocks or source facies, and showed evidence of biodegradation as suggested by the coexistence of n‐alkanes and 25‐norhopanes. The results indicate a close genetic relationship between the samples in the Carbonera (C7 interval), Mirador and Guadalupe Formation reservoirs. These petroleums are interpreted to result from at least two separate oil charges. An early charge (Oligocene to Early Miocene) was derived from marine carbonate and transitional siliciclastic Cretaceous source rocks as indicated by biomarker analysis using GC/MS. This initial oil charge was biodegraded in the reservoir, and was mixed with a later charge (or charges) of fresh oil during the Late Miocene to Pliocene. A relatively high proportion of the unaltered oil charge was recorded for heavy oil samples from the Melero‐1 well in the Tiple block, and is inferred to originate from Cenozoic carbonaceous shale or coaly source rocks. Geochemical parameters suggest that oils from the Gachetá and Une Formations are similar and that they originated from a source different to that of the other oil samples. These two oils do not correlate well with extracts from transitional siliciclastic source rock from the Upper Cretaceous Gachetá Formation in the Ramiriqui‐1 well, located in the LLA 22 block to the north. By contrast, one or more organofacies of the Gachetá Formation may have generated the heavy oil and most of the liquid oil samples. The results suggest that the heavy oils may have formed as a result of biodegradation at the palaeo oil‐water contact, although deasphalting cannot entirely be dismissed.     

Tunable Visual Detection of Dew by Bare Artificial Opals

The photonic properties of artificial opals directly depend on the atmosphere (air, usually) filling the voids in the constitutive dielectric material. Here, a novel use of bare artificial silica opals (direct and inverse) is presented for straightforward water dew detection, in which the stop‐band exhibits a drastic change upon soaking of the voids. In particular, the opal reflectance drops to one‐fifth within 100 ms scale, so the fading of the opal structural color upon dew formation is evident for the naked eye. Moreover, the stop‐band redshifts up to 60 nm, leading, by convenient selection of the stop‐band wavelength, to perceptible color change. Due to the porous nature of the opal, the dew formation in the opal voids is shifted to lower saturation pressures compared to an open system. This is experimentally demonstrated by controlled reduction of the pores size, so that the advancement of the dew point temperature is tuned between 2.5° and 6.3°. The outstanding color change together with the easy fabrication (without postprocessing) and selectable size (from miniature to large‐area) make artificial opals cost‐effective and versatile visual dew detectors. The tunable advance of the opal response over the actual dew point is practical to adequately anticipate and prevent dew formation in neighboring surfaces. Furthermore, opals might serve as useful probe systems for the study of fundamental phenomena such as condensation of vapors in porous and particulate media.     

Processing of ultrasonic array signals for characterizing defects. Part I: signal synthesis

This work presents a novel procedure to characterize damage using an array of ultrasonic measurements in a generalized model-based inversion scheme, which integrates the complete information recorded from the measurements. In the past, we proposed some idealized nondestructive evaluation test methods with emphasis on the numerical results, but it is necessary to develop the techniques in greater detail in order to apply the techniques to real conditions. Our detection principle is based on the measurement and inversion of frequency-domain data combined with a reduced set of output parameters. The approach is developed and tested for the case of an aluminum specimen with a synthetic array of point contact ultrasonic transmitters and receivers. The first part of this two-part paper is focused on numerical synthesis of the experimental measurements using the boundary element method for a general ultrasonic propagation model. This part also deals with the deconvolution by comparing the data measured from the damaged and undamaged specimens. The deconvolution technique allows us to calibrate the data by taking into account the uncertainties due to mechanical properties, input signal, and other coherent noise. The second part of the paper presents the inversion of the measurements to obtain the parameters and ultimately to predict the position and size of the real defect.     

Comparison of peristrophic multiplexing and a combination of angular and peristrophic holographic multiplexing in a thick PVA/acrylamide photopolymer for data storage

Two different types of multiplexing are used to store 90 holograms at the same location in a polyvinyl alcoholacrylamide photopolymer material. In the first, the 90 holograms are stored using only peristrophic multiplexing, whereas in the second a combination of angular and peristrophic multiplexing is used. The results (diffraction efficiency and dynamic range, M#) obtained with these two multiplexing techniques are compared. With the first, the dynamic range was M# = 13 and with the second M# = 8. An exposure schedule method is used to calculate the exposure time necessary to store the holograms with a more uniform, higher diffraction efficiency.     

Neutron fluence rate measurement using prompt gamma rays

A gamma ray spectrometer, with a 3(') ? X 3(') NaI(Tl) detector, with a moderator sphere has been utilised to measure the neutron fluence rate, with this value the H(10) was estimated. When a neutron is captured by the hydrogen-based moderator, a 2.22 MeV prompt gamma ray is produced. In a multichannel analyser the net area under the 2.22 MeV photopeak is proportional to the total neutron fluence rate. The features of this system were determined by a Monte Carlo study that includes 3-, 5- and 10-inches diameter, water and polyethylene moderators and a (239)Pu-Be source. The prompt gamma response was extended to monoenergetic neutron sources. To verify the response, a (239)Pu-Be source in combination with a 10(') polyethylene sphere having a gamma-ray spectrometer with NaI(Tl) was utilised to estimate the neutron fluence rate and the H(10). These results were compared with neutron fluence rate and H(10) obtained using a Bonner sphere spectrometer and with the H(10) measured using a neutron remmeter.     

Artificial neural networks technology for neutron spectrometry and dosimetry

Artificial Neural Network Technology has been applied to unfold neutron spectra and to calculate 13 dosimetric quantities using seven count rates from a Bonner Sphere Spectrometer with a (6)LiI(Eu). Two different networks, one for spectrometry and another for dosimetry, were designed. To train and test both networks, 177 neutron spectra from the IAEA compilation were utilised. Spectra were re-binned into 31 energy groups, and the dosimetric quantities were calculated using the MCNP code and the fluence-to-dose conversion coefficients from ICRP 74. Neutron spectra and UTA4 response matrix were used to calculate the expected count rates in the Bonner spectrometer. Spectra and H(10) of (239)PuBe and (241)AmBe were experimentally obtained and compared with those determined with the artificial neural networks.     

Water-extended polyester neutron shield for a 252Cf neutron source

A Monte Carlo study to determine the shielding features to neutrons of water-extended polyester was carried out. During calculations, (252)Cf and shielding were modelled and the neutron spectra as well as the H(10) were calculated in four sites. The calculation was extended to include a water shielding, the source in vacuum and in air. Besides neutron shielding characteristics, the Kerma in air due to gammas emitted by (252)Cf and due to capture gamma rays in the shielding were included.     

High‐Performance Thiol–Ene Composites Unveil a New Era of Adhesives Suited for Bone Repair

The use of adhesives for fracture fixation can revolutionize the surgical procedures toward more personalized bone repairs. However, there are still no commercially available adhesive solutions mainly due to the lack of biocompatibility, poor adhesive strength, or inadequate fixation protocols. Here, a surgically realizable adhesive system capitalizing on visible light thiol–ene coupling chemistry is presented. The adhesives are carefully designed and formulated from a novel class of chemical constituents influenced by dental resin composites and self‐etch primers. Validation of the adhesive strength is conducted on wet bone substrates and accomplished via fiber‐reinforced adhesive patch (FRAP) methodology. The results unravel, for the first time, on the promise of a thiol–ene adhesive with an unprecedented shear bond strength of 9.0 MPa and that surpasses, by 55%, the commercially available acrylate dental adhesive system Clearfil SE Bond of 5.8 MPa. Preclinical validation of FRAPs on rat femur fracture models details good adhesion to the bone throughout the healing process, and are found biocompatible not giving rise to any inflammatory response. Remarkably, the FRAPs are found to withstand loads up to 70 N for 1000 cycles on porcine metacarpal fractures outperforming clinically used K‐wires and match metal plates and screw implants.