Age-related differences in management of heart disease: a study of cardiac medication use in an older cohort. Pacemaker Selection in the Elderly (PASE) Investigators

Bone mass, bone metabolic markers, and calcium regulation hormones were measured in members of an Antarctic wintering team who stayed at the Japanese Antarctic station, Syowa (latitude: south 69 degrees 00', longitude: east 39 degrees 35') for 1 year. Subjects included 31 healthy Japanese males, aged 24-51 years (mean age 34.5 years) at the beginning of this study, ingesting 488 IU/day of vitamin D and 550.9 mg/day of calcium per person. The long-term coefficient of variation (CV) of the equipment used in the assessments of bone mass was 0.67% in single X-ray absorptiometry (SXA), 0.17% in the speed of sound (SOS) by quantitative ultrasound method (QUS), and 0.63% in broadband ultrasound attenuation (BUA) by QUS. The seasonal changes in the calcaneal bone mineral density (BMD) by SXA were not significant, whereas the SOS measured by QUS decreased during the measurement period (0.55%, p < 0.001), and BUA increased (1.9%, p < 0.01). Bone-specific alkaline phosphatase and osteocalcin levels increased significantly during summer (p < 0.001) and urinary calcium level decreased significantly during winter (p < 0.05). Urinary pyridinoline and deoxypyridinoline levels decreased significantly at the end of winter (p < 0.001). Serum 1,25(OH)2D3 level did not change significantly, whereas serum 25(OH)D3 level decreased significantly during winter (p < 0.001). Serum parathyroid hormone (PTH) level significantly increased at the end of winter (p < 0.01), although both PTH level and 25(OH)D3 level remained within the normal range. We concluded that the 25(OH)D3 level in subjects who stayed in Antarctica for 1 year decreased significantly with the reduction in duration of sunshine, but there were no clear changes in bone mass.     

Differential scanning microcalorimetry indicates that human defensin, HNP-2, interacts specifically with biomembrane mimetic systems

alpha-Defensins are antimicrobial peptides with 29-35 amino acid residues and cysteine-stabilized amphiphilic, triple-stranded beta-sheet structures. We used high-precision differential scanning microcalorimetry to investigate the effects of a human neutrophil alpha-defensin, HNP-2, on the phase behavior of model membranes mimicking bacterial and erythrocyte cell membranes. In the presence of this positively charged peptide, the phase behavior of liposomes containing negatively charged phosphatidylglycerol was markedly altered even at a high lipid-to-peptide molar ratio of 500:1. Addition of HNP-2 to liposomes mimicking bacterial membranes (mixtures of dipalmitoylphosphatidylglycerol and -ethanolamine) resulted in phase separation owing to some domains being peptide-poor and others peptide-rich. The latter are characterized by an increase of the main transition temperature, most likely arising from electric shielding of the phospholipid headgroups by the peptide. On the other hand, HNP-2 did not affect the phase behavior of membranes mimicking erythrocyte membranes (equimolar mixtures of dipalmitoylphosphatidylcholine and sphingomyelin) as well as the pure single components. This is in contrast to melittin, which significantly affected the phase behavior of choline phospholipids in accordance with its unspecific lytic activity. These results support the hypothesis of preferential interaction of defensins with negatively charged membrane cell surfaces, a common feature of bacterial cell membranes, and demonstrate that HNP-2 discriminates between model membrane systems mimicking prokaryotic and eukaryotic cell membranes.     

A multibus train communication (AMTRAC) architecture for high-speedfiber optic networks

A multibus train (ordered demand assignment) communication architecture, using the AMTRAC protocol (for efficient utilization of fiber-optic-based very-high-speed networks) is presented. Taking advantage of the emerging WDM (wavelength-division multiplexing) and FDM (frequency-division multiplexing) technologies, the proposed solution introduces a coordinated multichannel control combining the performance advantages of two known approaches for high-speed communication: multichannel and train protocols. As a result an AMTRAC-based high-speed network achieves channel utilization significantly higher than previous approaches. For a network consisting of N stations, with propagation delay to packet transmission time ratio given by a, the AMTRAC architecture reaches a capacity of 1/(1+a/N ²)     

Analysis of multiple-bus synchronous and asynchronous communication systems

We consider a multi-user distributed system in which nodes are connected to multiple-buses using partially duplicated bus interfaces. We study the effects of destination conflicts and bus synchronization on the system performance and show how, for a given system bus duplication can be chosen to provide guaranteed delays while increasing the interconnection system reliability. Alternative system configurations are analyzed and compared using exact and approximate Markovian models. The presented results can be used for design and optimization of high-availability distributed systems, such as loosely coupled cluster systems or backend storage networks.     

Channel allocation protocols in frequency-time controlled highspeed networks

In conventional high-speed systems, the high ratio between the end-to-end propagation delay and the message transmission time severely restricts the system performance. Thus, the increase in channel bandwidth may be accompanied by only a marginal increase in actual system capacity. A combined frequency-time division-based control of the high-speed channel that significantly reduces this problem is proposed. The design of protocols subject to the unique channel control penalties of the resulting multichannel system is considered. The allocation of channels on a demand assignment basis is hindered in the multichannel configuration by the time penalty involved in locating an idle channel and by practical limitations on the transmit/receive multichannels mode interface. A new class of fixed allocation protocols is introduced in which the channel access is obtained without the aforementioned penalties. The protocols build on the allocation of source and destination oriented transmission rights, taking into account the implementation aspects of multichannel networks. It is shown that this class of protocols covers the whole range of random-access to fixed-channel-access control policies. An analytic approach for a uniform analytic treatment is introduced, showing the potential for a significant improvement in the system capacity and the average message delay     

Validation of object-induced MR distortion correction for framelessstereotactic neurosurgery

Spatial fidelity is a paramount issue in image guided neurosurgery. Until recently, three-dimensional computed tomography (3D CT) has been the primary modality because it provides fast volume capture with pixel level (1 mm) accuracy. While three-dimensional magnetic resonance (3D MR) images provide superior anatomic information, published image capture protocols are time consuming and result in scanner- and object-induced magnetic field inhomogeneities which raise inaccuracy above pixel size. Using available scanner calibration software, a volumetric algorithm to correct for object-based geometric distortion, and a Fast Low Angle SHot (FLASH) 3D MR-scan protocol, the authors were able to reduce mean CT to MR skin-adhesed fiducial marker registration error from 1.36 to 1.09 mm. After dropping the worst one or two of six fiducial markers, mean registration error dropped to 0.62 mm (subpixel accuracy). Three dimensional object-induced error maps present highest 3D MR spatial infidelity at the tissue interfaces (skin/air, scalp/skull) where frameless stereotactic fiducial markers are commonly applied. The algorithm produced similar results in two patient 3D MR-scans     

Defensin stimulates the binding of lipoprotein (a) to human vascular endothelial and smooth muscle cells

There is evidence to suggest that elevated plasma levels of lipoprotein (a) [Lp(a)] represent a risk factor for the development of atherosclerotic vascular disease, but the mechanism by which this lipoprotein localizes to involved vessels is only partially understood. In view of studies suggesting a link between inflammation and atherosclerosis and our previous finding that leukocyte defensin modulates the interaction of plasminogen and tissue-type plasminogen activator with cultured human endothelial cells, we examined the effect of this peptide on the binding of Lp(a) to cultured vascular endothelium and vascular smooth muscle cells. Defensin increased the binding of Lp(a) to endothelial cells approximately fourfold and to smooth muscle cells approximately sixfold. Defensin caused a comparable increase in the amount of Lp(a) internalized by each cell type, but Lp(a) internalized as a consequence of defensin being present was not degraded, resulting in a marked increase in the total amount of cell-associated lipoprotein. Abundant defensin was found in endothelium and in intimal smooth muscle cells of atherosclerotic human cerebral arteries, regions also invested with Lp(a). These studies suggest that defensin released from activated or senescent neutrophils may contribute to the localization and persistence of Lp(a) in human vessels and thereby predispose to the development of atherosclerosis.     

Effect of transient abrupt vessel closure during otherwise successful angioplasty for unstable angina on clinical outcome at six months. Hirulog Angioplasty Study Investigators

BACKGROUND: A phase II trial was conducted to assess the efficacy of infusions of dendritic cells (DC) and two HLA-A2-specific PSMA peptides (PSM-P1 and -P2). This report describes thirty three subjects with hormone-refractory metastatic prostate cancer without prior vaccine therapy history who were evaluated and reported as a group. METHODS: All subjects received six infusions of DC pulsed with PSM-P1 and -P2 at six week intervals. Clinical monitoring was conducted pre-, during, and post- phase II study. Data collected include: complete blood count, bone and total alkaline phosphatase, prostate markers, physical examination, performance status, bone scan, ProstaScint scan, chest x-ray, as well as assays to monitor cellular immune responses. RESULTS: Six partial and two complete responders were identified in the phase II study based on NPCP criteria, plus 50% reduction of prostate-specific antigen (PSA), or resolution in previously measurable lesions on ProstaScint scan. CONCLUSIONS: Over 30% of study participants in this group showed a positive response at the conclusion of the trial. This study suggested that DC-based cancer vaccines may provide an alternative therapy for prostate cancer patients whose disease no longer responds to hormone therapy.     

Quality of Service Support for Multimedia Applications in Third Generation Mobile Networks Using Adaptive Scheduling

Third generation mobile network will support services such as video-telephony, video-conferencing and other multimedia applications. Therefore, this network must provide quality of service (QoS) to these applications consistent with that offered by fixed networks. However, this is a very challenging task due to the instability of the wireless channel and the diverse quality of service requirements dictated by different multimedia applications. In this paper we introduce a resource allocation algorithm for the wireless downlink that takes into account the wireless channel characteristics, the QoS required by the applications as well as a pricing value function. Our solution is based on an adaptive scheduling algorithm originally developed for scheduling real-time processes during transient surges. This algorithm tends to maximize the wireless network operator profit while satisfying the customers' quality requests.