Molecular Identification of the G-Protein Genes and Their Expression Profiles in Response to Nitrogen Deprivation in Brassica napus

Heterotrimeric guanine nucleotide binding protein (G-protein) consisting of Gα, Gβ, and Gγ subunits is one of the key signal transducers in plants. Recent studies indicated that G-protein has been proposed as an important mediator of nitrogen responses in rice, wheat, and Arabidopsis. However, little is known about these G-proteins in Brassica napus (B. napus), except for three identified G-proteins, BnGA1, BnGB1, and BnGG2. Therefore, the aim of the present study is to characterize the members of the G-protein gene family in allotetraploid B. napus and to analyze their expression profiles in response to nitrogen deprivation. In total, 21 G-protein family members were identified in B. napus, encoding two Gα, six Gβ, and 13 Gγ. Sequence and phylogenetic analyses showed that although genome-wide triploid events increased the number of genes encoding Gα, Gβ, and Gγ subunits, the gene structure and protein properties of the genes encoding each G-protein subunit were extremely conserved. Collinearity analysis showed that most G-protein genes in B. napus had syntenic relationships with G-protein members of Arabidopsis, Brassica rape (B. rapa), and Brassica oleracea (B. oleracea). Expression profile analysis indicated that Gα and C-type Gγ genes (except BnGG10 and BnGG12 were highly expressed in flower and ovule) were barely expressed in most organs, whereas most Gβ and A-type Gγ genes tended to be highly expressed in most organs. G-protein genes also showed various expression patterns in response to nitrogen-deficient conditions. Under nitrogen deficiency, Gα and five C-type Gγ genes were upregulated initially in roots, while in leaves, Gα was downregulated initially and five C-type Gγ genes were highly expressed in different times. These results provide a complex genetic dissection of G-protein genes in B. napus, and insight into the biological functions of G-protein genes in response to nitrogen deficiency.     

紫外分光光度法测定水溶液中的磺酸盐

文章研究了紫外分光光度法测量水相中磺酸盐浓度的方法。实验结果表明,磺酸盐的最大吸收波长均为225nm,单一体系和复配体系的工作曲线方程相同。研究了磺酸盐浓度、相态对测定结果的影响.实验表明,该方法重现性好,可操作性强,准确快速,可直接应用于10^7-10^-4M的磺酸盐浓度测定。     

某待建滨海核电厂厂址年均大气扩散因子计算模式与参数的实验研究

本文在某待建滨海核电厂厂趣现场野外实验基础上提出了计算该核电厂年均大气扩散因子的计算模型。此模型既考虑了向岸流与离岸流的差别,又考虑了混合层与热内边界层,混合释放与扩散参数随陆距变化等因素的综合影响。模型中所需要的各类参数,包括在１９９５,１９９６年间在该厂趣野外大气试验中获取的百米气象铁塔４个不同高度上的风,温逐时三维联合频率;湍流测量结合风洞实验获取的离岸流与向岸流的扩散参数。     

LD31合金高压瓶衬旋压成形组织与性能

ＬＤ３１合金瓶衬经冷热加工成形,其显微组织和力学性能不断改善,有助于获取优质高压气瓶。ＬＤ３１合金毛坯不及热开坯旋压厚壁管,为大有率的冷旋薄壁管创造了条件。ＬＤ３１冷旋性能良好,累计变薄率达７０％,其形变强化明显。加热缩旋的瓶衬经淬火时效后,Ｍｇ２Ｓｉ强化相弥散析出,相变强化显著,综合性能尚好,有利于提高玻璃纤维复合缠绕高压气瓶的使用性能。     

基于有限元法的柔性转子虚拟动平衡研究

为了有效地降低回转机械由于质量不平衡引起的振动故障，基于转子动力学理论和有限元数值分析方法，进行了柔性转子系统虚拟动平衡研究。提出了若干种降低系统振动的平衡方案，并分别对平衡结果进行了有限元数值仿真，以寻找最优的平衡方案。实验验证了虚拟动平衡提出的最优平衡方案，该方法可最大限度地降低旋转机械系统实际动平衡过程的启动试机次数。     

AD-15粉剂在电炉冶炼上的应用

AD-15脱氧粉剂在应用于生产后产生良好的效果,提高了经济效益,降低了各种消耗指标。     

丘陵山区波涌沟灌技术要素分析

针对丘陵山区地形和水资源短缺等特点,采用正交试验设计进行波涌沟灌田间试验.基于田间实测的灌水沟水流推进时间、消退时间和流量,着重分析了波涌沟灌技术要素对灌水质量、储水效率、灌水效率和灌水均匀度的影响.确定了技术要素和灌水质量的关系.为进一步得出波涌沟灌最佳灌水质量的技术要素组合奠定了基础.     

Inhibition of copper corrosion in aerated hydrochloric acid solution by heterocyclic compounds containing a mercapto group

Inhibition of copper corrosion by benzotriazole (BTA), 2-mercapto benzoxazole (MBO) and 2-mercapto benzimidazole (MBI) in 0.5 mol L⁻¹ HCl was investigated by weight-loss measurements, potentiodynamic polarization curves and electrochemical impedance spectroscopy. MBI was shown to be the most effective inhibitor among those tested. Potentiodynamic polarization results revealed that the three compounds acted as anodic inhibitors, particularly MBI strongly suppressed anodic current densities. Molecular structure parameters of BTA, MBO and MBI were obtained by using an MM2 forcefield program and PPP–SCF quantum chemical calculation. It was found that MBI has higher levels of HOMO and LUMO energy and the larger π-electron density.